A2Ar-dependent PD-1+ and TIGIT+ Treg cells have distinct homing requirements to suppress autoimmune uveitis in mice

Peters, Kayleigh; McDonald, Trisha; Muhammad, Fauziyya; Walsh, Marisa; Drenen, Kayla; Montieth, Alyssa; Foster, C. Stephen; Lee, Darren J.

doi:10.1016/j.mucimm.2023.04.005

Cited by 7 publications

(1 citation statement)

References 30 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a co-inhibitory molecule, TIGIT plays an important role in the function, proliferation and differentiation of T cells [ 15 ], including mTh17 and mTreg cells. TIGIT has the ability to enhance immunosuppression in Treg cells, and elevated levels of TIGIT + Treg cells have been known to suppress Th1 and Th17 cell responses [ 16 , 17 ]. TIGIT specifically binds to multiple ligands, including CD155, CD112 and CD113.…”

Section: Introductionmentioning

confidence: 99%

Astragalus Polysaccharide Alleviates Ulcerative Colitis by Regulating the Balance of mTh17/mTreg Cells through TIGIT/CD155 Signaling

Wan,

Huang,

Xiao

et al. 2024

Molecules

View full text Add to dashboard Cite

The balance between memory Th17 cells (mTh17) and memory Treg cells (mTreg) plays a key role in the pathogenesis of ulcerative colitis (UC), and TIGIT signaling is involved in the differentiation of mTh17/mTreg cells. Astragalus polysaccharide (APS) has good immunomodulatory and anti-inflammatory effects. Here, the regulatory effects and potential mechanisms of APS on mTh17/mTreg cells in UC are explored. A UC model was induced with dextran sulfate sodium (DSS) and treated simultaneously with APS (200 mg/kg/day) for 10 days. After APS treatment, the mice showed a significant increase in colonic length and a significant decrease in colonic weight, colonic weight index and colonic weight/colonic length, and more intact mucosa and lighter inflammatory cell infiltration. Notably, APS significantly down-regulated the percentages of Th17 (CD4+CCR6+), cmTh17 (CD4+CCR7+CCR6+) and emTh17 (CD4+CCR7−CCR6+) cells and significantly up-regulated the percentages of cmTreg (CD4+CCR7+Foxp3+) and emTreg (CD4+CCR7−Foxp3+) cells in the mesenteric lymph nodes of the colitis mice. Importantly, APS reversed the expression changes in the TIGIT molecule on mTh17/mTreg cells in the colitis mice with fewer CD4+CCR6+TIGIT+, CD4+CCR7−CCR6+TIGIT+ and CD4+CCR7−CCR6+TIGIT+ cells and more CD4+Foxp3+TIGIT+, CD4+CCR7−Foxp3+TIGIT+ and CD4+CCR7−Foxp3+TIGIT+ cells. Meanwhile, APS significantly inhibited the protein expression of the TIGIT ligands CD155, CD113 and CD112 and downstream proteins PI3K and AKT in the colon tissues of the colitis mice. In conclusion, APS effectively alleviated DSS-induced UC in mice by regulating the balance between mTh17/mTreg cells, which was mainly achieved through regulation of the TIGIT/CD155 signaling pathway.

show abstract

Section: Introductionmentioning

confidence: 99%