A41030, a complex of novel glycopeptide antibiotics produced by a strain of Streptomyces virginiae. Taxonomy and fermentation studies.

Abstract

A41030 is a complex of novel glycopeptide antibiotics produced by a culture isolated from a soil. Taxonomic studies have identified the microorganism, NRRL 15156, as a strain of Streptomyces virginiae. The major factor, A41030A, and three of the six minor factors are unique among glycopeptides in that they are naturally occurring aglycones, containing no neutral or amino sugars. The A41030 that was not spontaneously released into the fermentation broth could be released from the biomass into aqueous media at pH 10.5. In contrast to the vancomycin and N-demethylvancomycin fermentations, A41030 biosynthesis was stimulated by enriching the medium with KZHPO4 at a level of I mg/ml. Enrichment with putative precursors of the aglycone, however, did not increase the biosynthesis of A41030.

Growth and production kinetics of antimicrobial compound from Streptomyces albidoflavus 321.2

“…But the highest yield was recorded at 32°C; above this temperature growth, production was declined, indicating its mesophilic nature (Figure 2). Temperature suitability of streptomycetes was also observed as 32°C (Boeck et al, 1985). For the successful fermentation experiment, inoculum size is an important factor.…”

Scaling up a virginiamycin production by a high-yield Streptomyces virginiae VKM Ac-2738D strain using adsorbing resin addition and fed-batch fermentation under controlled conditions

“…Fructose and glucose were shown to be the best carbon sources for the S. virginiae HM3 strain (Rifaat and Kansoh 2005) and six other S. virginiae strains (Anzai et al 1994); in the last case, four strains were also able to utilize L-arabinose. Boeck et al (1985) described the S. virginiae NRRL 15156 strain able to grow on cellobiose, fructose, galactose, glucose, maltose, ribose, salicin, and succinate.…”

A gene cloning system for ‘Streptomyces toyocaensis’

“…'Streptomyces toyocaensis” produces antibiotic A47934 (Boeck & Mertz, 1986; Zmijewski et al ., 1987) and Streptomyces virginiae produces antibiotic A41030A (Boeck eta/ ., 1985). A47934 and A41030A contain the crosslinked aromatic heptapeptide structure common to actaplanin, teichoplanin, ristocetin and other glycopeptides (Lancini & Cavalleri, 1990).…”