Untitled

Abstract

For the present study, 10 bacterial strains were isolated from agriculture field soils collected from Guntur, Andhra Pradesh, India, and thereafter screened for amylase activity. Among them, the strain DS5 showed a maximum zone of clearance on the starch hydrolysis test was selected for this study. Identification of bacterial strain DS5 was confirmed by 16S rRNA sequencing analysis. The phylogenetic tree constructed on the basis of 16S rRNA sequences revealed that it clustered with the closest members of Bacillus pumilus DS5 and the sequences were deposited in NCBI Gen bank along with accession number MK430437. Further the effect of incubation period, temperature, pH, carbon and nitrogen sources was optimized. Highest amylase production was found after 48 h of incubation at pH 7.0 and 40 ⁰C temperature. Starch proved to be the best carbon source and peptone as nitrogen source for the highest production of amylase by Bacillus pumilus DS5. Supplementation with different nitrogen and carbon source favoured the increased enzyme yield by submerged fermentation.INTRODUCTION: Amylases are one of the prominent enzymes that hydrolysis starch molecules to give diverse products, including dextrin and progressively smaller polymers composed of glucose units 1 . Hydrolytic enzymes like alpha-amylase, lipase, Chitinase protease and Cellulases were obtained from microorganisms. However, amylases from bacillus species are ubiquitous; non-fastidious nutritional requirements and high productivity of most of the alphaamylases were organism choice 2, 3 . Amylases are among the most important enzyme and account for about 30% of the world's enzyme production 4 .