AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua

Abstract

The phytohormone Abscisic acid (ABA) regulates plant growth, development, and responses to abiotic stresses, including senescence, seed germination, cold stress and drought. Several kinds of researches indicate that exogenous ABA can enhance artemisinin content in A. annua. Some transcription factors related to ABA signaling are identified to increase artemisinin accumulation through activating the artemisinin synthase genes. However, no prior study on ABA transporter has been performed in A. annua. Here, we identified a pleiotropic drug resistance (PDR) transporter gene AaPDR4/ AaABCG40 from A. annua. AaABCG40 was expressed mainly in roots, leaves, buds, and trichomes. GUS activity is primarily observed in roots and the vascular tissues of young leaves in proAaABCG40: GUS transgenic A. annua plants. When AaABCG40 was transferred into yeast AD12345678, yeasts expressing AaABCG40 accumulated more ABA than the control. The AaABCG40 overexpressing plants showed higher artemisinin content and stronger drought tolerance. Besides, the expression of CYP71AV1 in OE-AaABCG40 plants showed more sensitivity to exogenous ABA than that in both wild-type and iAaABCG40 plants. According to these results, they strongly suggest that AaABCG40 is involved in ABA transport in A. annua.

植物abcg转运蛋白功能的研究进展

“…松布尔巴图 等人 [62] 研究发现, 盐芥(Eutrema salsugineum, 也称为 Thellungiella salsugineum)ABCG25在茎和根中的表达 水平非常高, 同时对外源ABA的响应显著, 推测 EsABCG25很可能通过直接参与盐芥对ABA信号的响 应进而导致盐芥对干旱胁迫的耐受. Fu等人 [63] 对黄花…”

Bacteria – Plant Interaction: Transgenic Roots for Human Health

“…Overexpression of artemisinic aldehyde Δ11 (13) reductase gene enhanced artemisinin and its relative metabolite biosynthesis in transgenic plants (Yuan et al, 2015). It was studied that A. annua plants overexpressing a pleiotropic drug resistance transporter gene AaABCG40 showed higher artemisinin content (Fu et al, 2020). Transgenic plants that carried the rol B gene showed a 1.2-12-fold increase in artemisinin, artesunate, and dihydroartemisinin content (Dilshad et al, 2015a).…”

Identification and expression analysis of ATP-binding cassette (ABC) transporters revealed its role in regulating stress response in pear (Pyrus bretchneideri)

“…Additionally, the ABCG36 gene in rice plays a crucial role in exporting toxic heavy metals such as arsenic from roots to shoots, thereby reducing their accumulation in edible parts of the plant [ 20 ]. In Artemisia annua , AaABCG40 enhances artemisinin content and modulates drought tolerance [ 30 ]. Furthermore, ABC transporters have been implicated in temperature stress responses.…”