AabHLH112, a bHLH transcription factor, positively regulates sesquiterpenes biosynthesis in Artemisia annua

Xiang, Lan; He, Pan; Shu, Guoping; Yuan, Mingyuan; Meng-ling, Wen; Lan, Xiaozhong; Liao, Zhihua; Tang, Yueli

doi:10.3389/fpls.2022.973591

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…α-Farnesene biosynthesis was mainly affected by the AFS genes in plant, and its expression was generally synchronous with its product (Du et al, 2022; Xiang et al, 2022). In this study, a DdeAFS gene was identified, which showed a significantly positive correlation with α-farnesene content, inferring that it was a key enzyme gene in α-farnesene biosynthesis in D. delavayi fruit.…”

Section: Discussionmentioning

confidence: 99%

Genome Assembly of Docynia delavayi Provides New Insights into α-Farnesene Biosynthesis

Tian,

Chen,

Jiang

et al. 2024

Preprint

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Docynia delavayi is an economically significant fruit species with a high market potential due to the special aroma of its fruit. Here, a 653.34 Mb high-quality genome of D. delavayi was first reported, of which 93.8% of the sequences were assembled to 17 chromosomes. A total of 48325 protein-coding genes were annotated in D. delavayi genome. Ks analysis proved that two whole genome duplication (WGD) events occurred in D. delavayi, resulting in the expansion of genes associated with terpene biosynthesis, which promoted its fruit-specific aroma production. Combined multi-omics analysis, α-farnesene was detected as the most abundant aroma substance emitted by D. delavayi fruit during storage, meanwhile a α-farnesene synthase gene and 15 transcription factors (TFs) potentially involved in α-farnesene biosynthesis were identified. Further studies for the regulation network of α-farnesene biosynthesis revealed that DdebHLH, DdeERF1 and DdeMYB could activate the transcription of DdeAFS. To our knowledge, it is the first report that MYB TF plays a regulatory role in α-farnesene biosynthesis, which will greatly facilitate future breeding programs for D. delavayi.

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Section: Discussionmentioning

confidence: 99%

Genome Assembly of Docynia delavayi Provides New Insights into α-Farnesene Biosynthesis

Tian,

Chen,

Jiang

et al. 2024

Preprint

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“…AabHLH112 acts as an indirect positive regulator of artemisinin via binding to the AaERF1 promoter [ 113 ]. A recent study showed AabHLH112 also played key roles in promoting the biosynthesis of other sesquiterpenes such as β-caryophyllene, epi-cedrol, and β-farnesene [ 78 ]. In addition to those positive regulators, AabHLH2 and AabHLH3, which belong to MYC-type bHLH TFs, were recently identified as negative regulators of artemisinin synthesis [ 79 ].…”

Section: Transcriptional Regulatory Networkmentioning

confidence: 99%

Advanced metabolic engineering strategies for increasing artemisinin yield in Artemisia annua L.

Li,

Yang,

et al. 2024

Horticulture Research

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Artemisinin, also known as “Qinghaosu”, is a chemically sesquiterpene lactone containing an endoperoxide bridge. Due to the high activity to kill Plasmodium parasites, artemisinin and its derivatives have continuously served as the foundation for antimalarial therapies. Natural artemisinin is unique to the traditional Chinese medicinal plant Artemisia annua L., and its content in this plant is low. This has motivated the synthesis of this bioactive compound using yeast, tobacco and Physcomitrium patens systems. However, the artemisinin production in these heterologous hosts is low and cannot fulfil its increasing clinical demand. Therefore, A. annua plants remain the major source of this bioactive component. Recently, the transcriptional regulatory networks related to artemisinin biosynthesis and glandular trichome formation have been extensively studied in A. annua. Various strategies including 1) enhancing the metabolic flux in artemisinin biosynthetic pathway, 2) blocking competition branch pathways, 3) using transcription factors (TFs), 4) increasing peltate glandular secretory trichome (GST) density, 5) applying exogenous factors, and 6) phytohormones have been used to improve artemisinin yields. Here we summarize recent scientific advances and achievements in artemisinin metabolic engineering, and discuss prospects in the development of high-artemisinin yielding A. annua varieties. This review provides new insights into revealing the transcriptional regulatory networks of other high value plant-derived natural compounds (e.g., taxol, vinblastine and camptothecin), as well as glandular trichome formation. It is also helpful for the researchers who intend to promote natural compounds production in other plants species.

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“…BIGPETALp, another gene encoding bHLH TF, regulates petal growth by interfering in postmitotic cell expansion [31]. Recent research has identified AabHLH112 as a positive regulator of sesquiterpenes biosynthesis (β-caryophyllene, epi-cedrol, and β-farnesene) in Artemisia annua L. (Asteraceae) [32]. In Solanum lycopersicum L. (Solanaceae), knockdown of SlMYC1 resulted in the reduction in monoterpene content in leaf and stem trichome and sesquiterpenes (β-caryophyllene and α-humulene) in leaf trichome, while the content of β-caryophyllene and α-humulene increased in stem trichome, indicating differential regulation by SlMYC1 [33].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the bHLH Transcription Factor Family in Wintersweet (Chimonanthus praecox)

Kamran,

Fu,

Wang

et al. 2023

IJMS

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Wintersweet (Chimonanthus praecox (L.) Link, Calycanthaceae) is an esteemed ornamental flowering shrub known for its distinct blooming period in winter, vibrant color petals, and captivating floral fragrance. Basic helix-loop-helix (bHLH) transcription factors (TFs) play pivotal roles as key regulators in secondary metabolites biosynthesis, growth, and development in plants. However, the systematic analysis of the bHLH family members and their role in the regulation of floral traits in Wintersweet remains insufficiently understood. To bridge this knowledge gap, we conducted a comprehensive genome-wide analysis of the C. praecox bHLH (CpbHLH) gene family, identifying a total of 131 CpbHLH genes across 11 chromosomes. Phylogenetic analysis classified these CpbHLH genes into 23 subfamilies, wherein most members within the same subfamily exhibited analogous intron/exon patterns and motif composition. Moreover, the expansion of the CpbHLH gene family was primarily driven by segmental duplication, with duplicated gene pairs experiencing purifying selection during evolution. Transcriptomic analysis revealed diverse expression patterns of CpbHLH genes in various tissues and distinct stages of Wintersweet flower development, thereby suggesting their involvement in a diverse array of physiological processes. Furthermore, yeast 2-hybrid assay demonstrated interaction between CpbHLH25 and CpbHLH59 (regulators of floral scent and color) as well as with CpbHLH112 and CpMYB2, suggesting potential coordinately regulation of secondary metabolites biosynthesis in Wintersweet flowers. Collectively, our comprehensive analysis provides valuable insights into the structural attributes, evolutionary dynamics, and expression profiles of the CpbHLH gene family, laying a solid foundation for further explorations of the multifaceted physiological and molecular roles of bHLH TFs in Wintersweet.

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AabHLH112, a bHLH transcription factor, positively regulates sesquiterpenes biosynthesis in Artemisia annua

Cited by 6 publications

References 45 publications

Genome Assembly of Docynia delavayi Provides New Insights into α-Farnesene Biosynthesis

Genome Assembly of Docynia delavayi Provides New Insights into α-Farnesene Biosynthesis

Advanced metabolic engineering strategies for increasing artemisinin yield in Artemisia annua L.

Genome-Wide Identification and Expression Analysis of the bHLH Transcription Factor Family in Wintersweet (Chimonanthus praecox)

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