Ab0066 the Jak Inhibitor (Baricitinib) Inhibits Ifnγ-Induced Gliostatin Expression in Human Fibroblast-Like Synoviocytes

Abstract

Background: Rheumatoid arthritis (RA) is an autoimmune rheumatic disease. Activated B-and T-lymphocytes, mast cells, macrophages, tissue fibroblasts play a leading role in its pathogenesis. The development of autoimmune inflammation is impossible without the influence of a large number of pro-inflammatory cytokines such as IL 1α and β, TNF α, IL 6, IL 17, IL 22. Currently, other classes of biologically active molecules, such as adiponectin, visfatin, nesfatin, fetuin A, are being actively studied in RA [1,2,3,4]. Pre-B-cell colony enhancing factor 1 (PBEF1) stimulates synthesis of matrix metalloproteinases and chemokines, supporting synovial inflammation caused by leukocyte infiltration. A positive correlation between visfatin and C-reactive protein confirms its role as a mediator of inflammation. It is believed that increased concentrations of PBEF1 can stimulate systemic inflammation. Objectives: The study the relationship between serum PBEF1 level and disease activity in RA patients. Methods: We observed 140 patients with a reliable diagnosis of RA, of whom 96 were women and 44 were men. The control group consisted of 20 women and 10 men aged from 22 to 55 years without complaints of pain in the joints during life. PBEF1 concentration in blood serum were determined by indirect enzyme-linked immunosorbent assay using commercial test systems (RaiBiotech, cat№ EIA-VIS-1) according to the manufacturer's instructions. Results: The level of normal values of PBEF1 in healthy individuals with a BMI of 18.5 to 24.9 kg/m2 was 0.14-3.9 ng/ml, with a BMI of 25 to 29.9 kg/m 2 -0 -5.9 ng/ ml. Elevated serum PBEF1 level was detected in 84.29% of RA patients. The ones with elevated PBEF1 levels of significantly more likely to have a higher degree of activity index DAS28 (χ 2 =48,293; p<0,001,), high level of anti-cyclic citrullinated peptide (anti-CCP) (χ 2 =5,386; p=0,0203), higher levels of C-reactive protein (χ 2 =8,159; p=0,0043), erythrocyte sedimentation rate (p<0,001), extraarticular manifestations of the disease (χ 2 =7,354; p=0,0067). Conclusion: PBEF1 can be regarded as an important link in the pathogenesis of rheumatoid arthritis and for the potential molecule for biological therapeutic agents.