Point-of-care (POC) devices can provide inexpensive,
practical,
and expedited solutions for applications ranging from biomedicine
to environmental monitoring. This work reports on the development
of low-cost microfluidic substrates for POC systems suitable for analytical
assays, while also satisfying the need for social and environmentally
conscious practices regarding circular economy, waste reduction, and
the use of local resources. Thus, an innovative greener process to
extract cellulose from plants including abaca, cotton, kozo, linen,
and sisal, originating from different places around the world, is
developed, and then the corresponding paper substrates are obtained
to serve as platforms for POC assays. Hydrophobic wax is used to delineate
channels that are able to guide solutions into chambers where the
colorimetric assay for total cholesterol quantification is carried
out as a proof of concept. Morphological and physicochemical analyses
are performed, including the evaluation of fiber diameter, shape and
density, and mechanical and thermal properties, together with peel
adhesion of the printed wax channels. Contact angle and capillary
flow tests ascertain the suitability of the substrates for liquid
assays and overall viability as low-cost, sustainable microfluidic
substrates for POC applications.