AbaR4-Type Resistance Island Including the
            <i>bla</i>
            <sub>OXA-23</sub>
            Gene in Acinetobacter nosocomialis Isolates

Kim, Dae Hun; Choi, Ji Young; Jung, Sook-In; Thamlikitkul, Visanu; Song, Jae-Hoon; Ko, Kwan Soo

doi:10.1128/aac.00923-12

Antimicrob Agents Chemother

2012

DOI: 10.1128/aac.00923-12

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AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

Dae Hun Kim

Ji Young Choi

Sook-In Jung

et al.

Abstract: e This study reports for the first time the AbaR4-type resistance island with the bla OXA-23 gene in two carbapenem-resistant A. nosocomialis isolates from South Korea and Thailand.

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Cited by 12 publications

(18 citation statements)

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“…Besides sharing a common region (OXA-23-⌬ATPase), all the transposons have ISAba1 upstream from bla OXA-23 except Tn2007, which is correlated with ISAba4 (5, 9-11). Tn2006 and Tn2009 were also identified in non-baumannii species of Acinetobacter (7,8). Furthermore, the expression of bla OXA-23 has probably been enhanced by the strong promoters provided on the upstream insertion sequences of the transposons (12,13).…”

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confidence: 94%

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Dissemination of bla _OXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn 2009

Liu

Ruan

et al. 2015

Antimicrob Agents Chemother

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bProduction of the OXA-23 carbapenemase is the most common reason for the increasing carbapenem resistance in Acinetobacter spp. This study was conducted to reveal the genetic basis of bla OXA-23 dissemination in Acinetobacter spp. in China. A total of 63 carbapenem-resistant OXA-23-producing Acinetobacter sp. isolates, representing different backgrounds, were selected from 28 hospitals in 18 provinces for this study. Generally, two patterns of plasmids carrying bla OXA-23 were detected according to S1-nuclease pulsed-field gel electrophoresis and Southern blot hybridization. A ca. 78-kb plasmid, designated pAZJ221, was found in 23 Acinetobacter baumannii and three Acinetobacter nosocomialis isolates, while a novel ca. 50-kb plasmid was carried by only two other A. baumannii isolates. Three of these isolates had an additional copy of bla OXA-23 on the chromosome. Transformation of the two plasmids succeeded, but only pAZJ221 was conjugative. Plasmid pAZJ221 was sequenced completely and found to carry no previously known resistance genes except bla OXA-23 . The bla OXA-23 gene of the remaining 35 isolates was chromosome borne. The bla OXA-23 genetic environments were correlated with Tn2009 in 57 isolates, Tn2008 in 5 isolates, and Tn2006 in 1 isolate. The MIC values for the carbapenems with these isolates were not significantly associated with the genomic locations or the copy numbers of bla OXA-23 . Overall, these observations suggest that the plasmid pAZJ221 and Tn2009 have effectively contributed to the wide dissemination of bla OXA-23 in Acinetobacter spp. in China and that horizontal gene transfer may play an important role in dissemination of the bla OXA-23 gene.

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confidence: 94%

“…Moreover, the bla OXA-23 gene has also been detected in non-baumannii Acinetobacter spp. (7,8). Transposable elements have played an essential role in the dissemination of the bla gene between different genomic locations within one bacterium or between different isolates (5).…”

mentioning

confidence: 99%

Dissemination of bla _OXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn 2009

Liu

Ruan

et al. 2015

Antimicrob Agents Chemother

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“…baumannii species. The bla OXA-23 gene was found in A. pittii (Acinetobacter genomic species 3) in the Irish Republic in 2006 and in A. nosocomialis (Acinetobacter genomic species 13TU) in South Korea and Thailand in 2012 (7,8). Moreover, bla OXA-51 preceded by ISAba1 has been found in carbapenem-resistant A. nosocomialis in Taiwan (9).…”

mentioning

confidence: 99%

“…The presence of oxacillinase genes in non-A. baumannii isolates had already been described in studies from China, South Korea, and Singapore, which underscores the potential clinical significance of these species (7)(8)(9)15).…”

mentioning

confidence: 99%

First Report of Carbapenem-Resistant Acinetobacter nosocomialis Isolates Harboring IS Aba 1- bla _OXA-23 Genes in Latin America

Teixeira

Martins²,

Barin

et al. 2013

J Clin Microbiol

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In recent years, different resistance genes have been found in Acinetobacter spp., especially in the species A. baumannii. We describe two isolates of carbapenem-resistant A. nosocomialis harboring ISAba1-bla OXA-23 and bla OXA-51 found in patients from the city of Porto Alegre, southern Brazil. To the best of the authors' knowledge, this is the first report of carbapenem-resistant A. nosocomialis in Latin America. In recent years, Acinetobacter spp. have been described as important pathogens in outbreaks of nosocomial infection worldwide, especially in intensive care units (1). In particular, the species A. baumannii has presented an increased rate of antimicrobial resistance (2, 3). Carbapenems, once regarded as the treatment of choice for infections caused by Acinetobacter spp., are no longer effective in some cases (2). The main mechanism of carbapenem resistance among Acinetobacter spp. is the production of ␤-lactamases, in particular class D ␤-lactamases (oxacillinases), associated with promoter gene sequence ISAba1 (3). Among oxacillinases, the most prevalent one is bla , identified in mobile genetic elements. Chromosomally located bla OXA-51 genes, in turn, do not always confer carbapenem resistance but are used to identify A. baumannii, as it is believed to be intrinsic to this species (4-6).Traditionally, the bla OXA-23 and bla OXA-51 genes are associated with A. baumannii only, but recently some authors have described the presence of such genes in non-A. baumannii species. The bla OXA-23 gene was found in A. pittii (Acinetobacter genomic species 3) in the Irish Republic in 2006 and in A. nosocomialis (Acinetobacter genomic species 13TU) in South Korea and Thailand in 2012 (7, 8). Moreover, bla OXA-51 preceded by ISAba1 has been found in carbapenem-resistant A. nosocomialis in Taiwan (9).In this study, we evaluated a set of non-A. baumannii species and found two isolates of carbapenem-resistant A. nosocomialis with the ISAba1-bla OXA-23 and bla OXA-51 genes, obtained from patients living in the city of Porto Alegre, southern Brazil.A total of 118 isolates were evaluated, obtained over the year 2011 from clinical specimens of Acinetobacter spp. previously identified using conventional methods. Isolates were identified to the species level using gyrB multiplex PCR as described by Higgins et al., with few modifications (10). Briefly, we used seven primers at a total reaction volume of 25 l, consisting of 0.2 M each primer, 1.5 mM MgCl 2 , 1ϫ 0.2 mM each deoxynucleoside triphosphate (dNTP), and 1 U Taq DNA polymerase. The PCR program consisted of initial denaturation at 94°C for 2 min, followed by 30 cycles of denaturation (94°C for 1 min), annealing (56°C for 30 s), and extension (72°C for 1 min), with a final extension step at 72°C for 10 min. Species identification was also evaluated by PCR with primers targeting the 16S-23S rRNA intergenic transcribed spacer (ITS) region, followed by sequence analysis (11). Oxacillinase genes (bla OXA-23 , bla OXA-24 , bla OXA-51 , bla , and bla OXA-143 ) were ident...

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“…Some studies have revealed diverse related AbaR resistance islands in the same region of comM in A. baumannii isolates (4)(5)(6)(7)(8). Although most AbaR resistance islands have been reported in A. baumannii isolates, recently, AbaR4 was reported in A. nosocomialis isolates from South Korea and Thailand (9). However, the prevalence and characteristics of AbaR-type genomic islands (GIs) in non-baumannii Acinetobacter isolates are not well known.…”

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confidence: 99%

AbaR-Type Genomic Islands in Non-baumannii Acinetobacter Species Isolates from South Korea

Kim¹,

Ko²

2015

Antimicrob Agents Chemother

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To investigate the presence and structure of AbaR-type genomic islands (GIs) in non-Acinetobacter baumannii isolates, a total of 155 non-baumannii Acinetobacter isolates from a South Korean hospital were analyzed. GIs were found in three Acinetobacter nosocomialis and two Acinetobacter seifertii isolates. Their structures were similar to those in A. baumannii isolates from Asian countries, including South Korea. The existence of AbaR-type GIs in non-baumannii Acinetobacter isolates is believed to be due to interspecies transfer of GI.

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AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

Abstract: e This study reports for the first time the AbaR4-type resistance island with the bla OXA-23 gene in two carbapenem-resistant A. nosocomialis isolates from South Korea and Thailand.

Cited by 12 publications

References 11 publications

Dissemination of bla _OXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn 2009

Dissemination of bla _OXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn 2009

First Report of Carbapenem-Resistant Acinetobacter nosocomialis Isolates Harboring IS Aba 1- bla _OXA-23 Genes in Latin America

AbaR-Type Genomic Islands in Non-baumannii Acinetobacter Species Isolates from South Korea

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AbaR4-Type Resistance Island Including the bla OXA-23 Gene in Acinetobacter nosocomialis Isolates

Abstract: e This study reports for the first time the AbaR4-type resistance island with the bla OXA-23 gene in two carbapenem-resistant A. nosocomialis isolates from South Korea and Thailand.

Cited by 12 publications

References 11 publications

Dissemination of bla OXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn 2009

Dissemination of bla OXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn 2009

First Report of Carbapenem-Resistant Acinetobacter nosocomialis Isolates Harboring IS Aba 1- bla OXA-23 Genes in Latin America

AbaR-Type Genomic Islands in Non-baumannii Acinetobacter Species Isolates from South Korea

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AbaR4-Type Resistance Island Including the bla _OXA-23 Gene in Acinetobacter nosocomialis Isolates

Dissemination of bla _OXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn 2009

Dissemination of bla _OXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn 2009

First Report of Carbapenem-Resistant Acinetobacter nosocomialis Isolates Harboring IS Aba 1- bla _OXA-23 Genes in Latin America