Abattoir study on Fasciola gigantica in Cambodian cattle

Sothoeun, S.; Davun, H.; Copeman, B.

doi:10.1007/s11250-006-4302-y

Cited by 12 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chronic Fasciola spp. infection in animals and humans give variable egg outputs, therefore we optimised our workflow to maximise the analytical sensitivity to <10 EPG [ 9 , 25 – 27 ]. Our initial success with clean eggs demonstrated consistent DNA isolation, although applying this approach to naturally-infected faecal samples with low EPGs (<10 EPG) proved challenging.…”

Section: Discussionmentioning

confidence: 99%

“…gigantica egg in 100 mg faeces, equivalent to 10 EPG. Thus, Method 1 is of limited diagnostic use in chronically infected cattle that frequently report low egg numbers (≤10EPG) [ 25 , 27 ]. To improve the approach, a concentration step was included which employed the traditional sedimentation technique for trematode eggs [ 9 ] without the microscopic observation and counting (Method 3).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Scrambled eggs: A highly sensitive molecular diagnostic workflow for Fasciola species specific detection from faecal samples

et al. 2017

View full text Add to dashboard Cite

BackgroundFasciolosis, due to Fasciola hepatica and Fasciola gigantica, is a re-emerging zoonotic parasitic disease of worldwide importance. Human and animal infections are commonly diagnosed by the traditional sedimentation and faecal egg-counting technique. However, this technique is time-consuming and prone to sensitivity errors when a large number of samples must be processed or if the operator lacks sufficient experience. Additionally, diagnosis can only be made once the 12-week pre-patent period has passed. Recently, a commercially available coprological antigen ELISA has enabled detection of F. hepatica prior to the completion of the pre-patent period, providing earlier diagnosis and increased throughput, although species differentiation is not possible in areas of parasite sympatry. Real-time PCR offers the combined benefits of highly sensitive species differentiation for medium to large sample sizes. However, no molecular diagnostic workflow currently exists for the identification of Fasciola spp. in faecal samples.Methodology/Principal findingsA new molecular diagnostic workflow for the highly-sensitive detection and quantification of Fasciola spp. in faecal samples was developed. The technique involves sedimenting and pelleting the samples prior to DNA isolation in order to concentrate the eggs, followed by disruption by bead-beating in a benchtop homogeniser to ensure access to DNA. Although both the new molecular workflow and the traditional sedimentation technique were sensitive and specific, the new molecular workflow enabled faster sample throughput in medium to large epidemiological studies, and provided the additional benefit of speciation. Further, good correlation (R2 = 0.74–0.76) was observed between the real-time PCR values and the faecal egg count (FEC) using the new molecular workflow for all herds and sampling periods. Finally, no effect of storage in 70% ethanol was detected on sedimentation and DNA isolation outcomes; enabling transport of samples from endemic to non-endemic countries without the requirement of a complete cold chain. The commercially-available ELISA displayed poorer sensitivity, even after adjustment of the positive threshold (65–88%), compared to the sensitivity (91–100%) of the new molecular diagnostic workflow.Conclusions/SignificanceSpecies-specific assays for sensitive detection of Fasciola spp. enable ante-mortem diagnosis in both human and animal settings. This includes Southeast Asia where there are potentially many undocumented human cases and where post-mortem examination of production animals can be difficult. The new molecular workflow provides a sensitive and quantitative diagnostic approach for the rapid testing of medium to large sample sizes, potentially superseding the traditional sedimentation and FEC technique and enabling surveillance programs in locations where animal and human health funding is limited.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Scrambled eggs: A highly sensitive molecular diagnostic workflow for Fasciola species specific detection from faecal samples

et al. 2017

View full text Add to dashboard Cite

show abstract

“…This is primarily because, until recently, all molecular Fasciola spp. identification was restricted to the analysis of DNA from adult flukes collected from the livers of infected animals during postmortem examination [23][24][25]. A reliance on access to adult parasites for species identification via molecular methods precludes widespread surveillance in a system that inherently requires the movement of live animals.…”

Section: Open Accessmentioning

confidence: 99%

Section: Fasciola Spp Distribution In Southeast Asia As a Rmentioning

confidence: 99%

Fasciola Species Introgression: Just a Fluke or Something More?

Calvani

Šlapeta

2021

Trends in Parasitology

View full text Add to dashboard Cite

show abstract

“…Cattle and buffalo are the most susceptible species to HS, with vaccination the primary control method (Benkirane and De Alwis, ; Savoeurn et al., ). The main parasitic diseases in Cambodian cattle are fascioliasis, trypanosomiasis, toxocariasis, cysticercosis, strongyloidiasis, schistosomiasis, thelaziasis, oesophagostomiasis and diseases caused by ticks and flies (Inoue et al., ; Tum et al., ; Sothoeun et al., ; Suon et al., ; Dorny et al., ; Young et al., ).…”

Section: Introductionmentioning

confidence: 99%

Impacts on Rural Livelihoods in Cambodia Following Adoption of Best Practice Health and Husbandry Interventions by Smallholder Cattle Farmers

Young

O'Reilly

Ashley

et al. 2014

Transbound Emerg Dis

View full text Add to dashboard Cite

To better understand how smallholder farmers whom own the majority of Cambodian cattle can contribute to efforts to address food security needs in the Mekong region, a five-year research project investigating methods to improve cattle health and husbandry practices was conducted. Cattle production in Cambodia is constrained by transboundary animal diseases (TADs) including foot-and-mouth disease (FMD) and haemorrhagic septicaemia (HS) plus poor nutrition, reproduction and marketing knowledge. The project worked in six villages in Kandal, Takeo and Kampong Cham province during 2007-12. Farmers from three 'high intervention' (HI) villages incrementally received a participatory extension programme that included FMD and HS vaccination, forage development and husbandry training. Evaluation of project impacts on livelihoods was facilitated by comparison with three 'low intervention' (LI) villages where farmers received vaccinations only. Results of knowledge, attitude and practice (KAP) and socio-economic surveys conducted in 2012 of 120 participating farmers identified that farmer knowledge in the HI project sites exceeded LI sites on the topics of biosecurity, internal parasites, nutrition and reproduction. HI farmers adopted biosecurity practices including a willingness to vaccinate for FMD and HS at their own cost, separate sick from healthy cattle, grow and feed forages and displayed awareness of the benefits of building fattening pens. HI farmers that grew forages observed time savings exceeding two hours per day each for men, women and children, enabling expansion of farm enterprises, secondary employment and children's schooling. Logistic regression analysis revealed that farmers in the HI group significantly increased annual household income (P < 0.001), with 53% reporting an increase of 100% or more. We conclude that improving smallholder KAP of cattle health and production can lead to improved livelihoods. This strategy should be of interest to policymakers, donors, researchers and extension workers interested in addressing TAD control, food insecurity and rural poverty in Southeast Asia.

show abstract

Abattoir study on Fasciola gigantica in Cambodian cattle

Cited by 12 publications

References 4 publications

Scrambled eggs: A highly sensitive molecular diagnostic workflow for Fasciola species specific detection from faecal samples

Scrambled eggs: A highly sensitive molecular diagnostic workflow for Fasciola species specific detection from faecal samples

Fasciola Species Introgression: Just a Fluke or Something More?

Impacts on Rural Livelihoods in Cambodia Following Adoption of Best Practice Health and Husbandry Interventions by Smallholder Cattle Farmers

Contact Info

Product

Resources

About