Volatile metabolites synthesized by strains of Luctobacillus brevis, L. jkctivorans, L. hilgardii, L. plantamn, Pediococcus pan&us, and Leuconostoc oenos, originally isolated from commercial wines, were extracted from a synthetic medium using Cra sorbent cartridges and analyzed using gas-liquid chromatography. All organisms synthesized isoamyl alcohol, 1-hexanol, 3-(methylthio)-1-propanol, and 2-phenyl ethanol in varying concentrations. Several other alcohols, acids, aldehydes, and esters were produced or utilized by some species or strains and enabled differentiation of the organisms. Recoveries of metabolites were dependent on the compounds, matrix, and presence of ethanol.