ABC proteins activity and cytotoxicity in zebrafish hepatocytes exposed to triclosan

Guidony, Nicole Soares; Scaini, João L.; Oliveira, Matheus William Bandeira de; Machado, Karina Santos; Bastos, Claudio L.Q.; Escarrone, Ana Laura Venquiaruti; Souza, Maurício de

doi:10.1016/j.envpol.2020.116368

Cited by 12 publications

(5 citation statements)

References 49 publications

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“…The concentration dependent decrease in cell viability while increase in the percentage of necrotic and apoptotic cells confirm the cytotoxic potential of TCS and the observation is directly supported by Binelli et al (2009aBinelli et al ( , 2009b, Parenti et al (2019) and Guidony et al (2020) who observed similar effects of TCS in zebra mussel, zebrafish embryos and zebrafish hepatocytes, respectively. Apoptosis or necrosis is induced by TCS either due to its action as an intercalating agent (through dioxins, the degraded product of TCS) directly or through induction of oxidative stress indirectly (Aranami and Readman, 2007).…”

Section: Discussionsupporting

confidence: 67%

Cellular, molecular and genomic alterations in the hatchlings of Labeo rohita after exposure to Triclosan

Sharma

Dar

Andotra

et al. 2022

Front. Environ. Sci.

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Triclosan 5-chloro-2-(2, 4-dichlorophenoxy) phenol (TCS) is widely used as a biocide in human and veterinary medicines, personal care products and household articles. To obtain biomarkers for the acute stress of Triclosan, the hatchlings of Labeo rohita were exposed for 96 h to 0.06, 0.067 and 0.097 mg/L TCS. Morphological deformities, cell viability, frequency of micronucleated and aberrant cells, transcriptomic and biomolecular alterations were recorded after exposure and a depuration period of 10 days. The exposed hatchlings had a pointed head, curved trunk, lean body, deformed caudal fin, haemorrhage, hypopigmentation and tissue degeneration at 0.067 and 0.097 mg/L only. The frequency of viable cells declined but that of necrotic, apoptotic, micronucleated and abnormal cells increased (p ≤ 0.01) in a concentration dependent manner after exposure as well as the depuration period. After recovery, the frequency of viable and micronucleated cells increased, but that of necrotic, apoptotic, and aberrant cells declined in comparison to their respective 96 h values. The mRNA level of HSP47, HSP70, HSc71 and α-tropomyosin increased (p ≤ 0.01), while that of HSP60, HSP90, DHPR, myosin light polypeptide 3, desmin b and lamin b1 declined (p ≤ 0.01) after exposure. Ten days post exposure, a significant increase (p ≤ 0.01) over control was observed in the expression of all the heat shock and cytoskeletal genes and the values (except for HSc71) were higher than the respective 96 h values also. Infrared spectra showed that band area of amide A, amide I, amide II and phospholipids increased significantly (p ≤ 0.01) but peak intensity of lipid, glycogen and nucleic acids decreased after exposure. After recovery, area of the peaks for most of the biomolecules [except lipids (2924–2925, 1455–1457 cm−1) and glycogen (1163–1165 cm−1)] declined significantly over control and 96 h values. Collectively these changes seem to be responsible not only for the onset of paralysis but also for the concentration dependent increase in larval and cellular abnormalities as well as no/sporadic swimming movement in exposed hatchlings. It is evident that HSP60, HSc71, HSP90, α-tropomyosin and DHPR were strongly affected but DHPR can be used as the most sensitive marker for the toxicity of TCS. This is the first study reporting effect of TCS on the selected heat shock and cytoskeletal genes in a single model.

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Section: Discussionsupporting

confidence: 67%

Cellular, molecular and genomic alterations in the hatchlings of Labeo rohita after exposure to Triclosan

Sharma

Dar

Andotra

et al. 2022

Front. Environ. Sci.

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“…The effective antimicrobial concentrations all fell above the IC 50 values. Triclosan has been shown to be cytotoxic through impairment of mitochondrial activity, loss of membrane stability, and is thought to lead to direct cell apoptosis [52]. Both triclosan and cinnamaldehyde have poor solubility; the combination of both agents may antagonize this effect and lead to poor bioavailability of the active agents, hence the antagonistic effects observed in both cytotoxicity and antimicrobial assays.…”

Section: Table Imentioning

confidence: 99%

Antimicrobial and cytotoxic synergism of biocides and quorum-sensing inhibitors against uropathogenic Escherichia coli

Capper-Parkin¹,

Nichol²,

Smith³

et al. 2023

Journal of Hospital Infection

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“…These environmental factors may cause hearing loss by damaging hair cells in living organisms [6]. The risks of deodorant usage have been reviewed for various organs, including the liver, skeletal muscle, endocrine system, and other metabolic processes [7][8][9][10][11][12]. However, there is insufficient research regarding the effects of deodorants on hair cell damage or ototoxicity.…”

Section: Introductionmentioning

confidence: 99%

Exploring Embryo-Ototoxic Effects: Insights into Deodorant-Induced Hair Cell Damage in Zebrafish

Yoon,

Hyun,

Hong

et al. 2024

IJMS

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Our study investigated the embryo-ototoxic effects of deodorant2 (DA2) on zebrafish embryos, which serve as valuable model organisms due to genetic and physiological similarities to humans. We focused on understanding DA2’s impact on zebrafish hair cells, which are vital for sensory perception and balance regulation. DA2, provided by the Ministry of Environment, Republic of Korea, was used at 460 μg/mL in dimethyl sulfoxide (DMSO), with a 0.43% DMSO solvent control group. Three experiments, each using 10 zebrafish specimens from each group, showed an initial 13% hair cell count reduction in the DA2-exposed group. Subsequent experiments demonstrated reductions of 37% and 22%, each with one mortality case. Statistical analysis revealed a significant 24% hair cell count reduction in the DA2-exposed group. We also assessed DA2’s impact on zebrafish behavior. Although not statistically significant, differences in distances traveled (0.33–0.39, 95% confidence interval: −0.46–1.1, p = 0.2033) and latencies (−0.016–0.018, 95% confidence interval: −0.052–0.021, p = 0.1917) hinted at negative effects. These results highlight DA2’s ototoxic properties affecting zebrafish auditory systems and behavior. Further investigation into DA2’s effects on aquatic organisms and potential mitigation strategies are essential. These findings contribute to understanding DA2’s safety profile, benefiting aquatic ecosystems and human health assessments.

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ABC proteins activity and cytotoxicity in zebrafish hepatocytes exposed to triclosan

Cited by 12 publications

References 49 publications

Cellular, molecular and genomic alterations in the hatchlings of Labeo rohita after exposure to Triclosan

Cellular, molecular and genomic alterations in the hatchlings of Labeo rohita after exposure to Triclosan

Antimicrobial and cytotoxic synergism of biocides and quorum-sensing inhibitors against uropathogenic Escherichia coli

Exploring Embryo-Ototoxic Effects: Insights into Deodorant-Induced Hair Cell Damage in Zebrafish

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