ABCI, the mammalian homologue of the engulfment gene ced-7, is required during phagocytosis of both necrotic and apoptotic cells

Moynault, Alix; Luciani, Marie‐Françoise; Chimini, Giovanna

doi:10.1042/bst0260629

Cited by 29 publications

(18 citation statements)

References 14 publications

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“…The function of ABCA1 is not confined to transport of cholesterol and phospholipid: the protein is also functional in phagocytosis (22,23). Macrophages from Abca1 -/-mice were shown to be impaired in engulfment of apoptotic bodies (22,23).…”

Section: Introductionmentioning

confidence: 99%

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Hepatobiliary cholesterol transport is not impaired in Abca1-null mice lacking HDL

Groen¹,

Bloks²,

Bandsma³

et al. 2001

J. Clin. Invest.

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The ABC transporter ABCA1 regulates HDL levels and is considered to control the first step of reverse cholesterol transport from the periphery to the liver. To test this concept, we studied the effect of ABCA1 deficiency on hepatic metabolism and hepatobiliary flux of cholesterol in mice. Hepatic lipid contents and biliary secretion rates were determined in Abca1 -/-, Abca1 +/-, and Abca1 +/+ mice with a DBA background that were fed either standard chow or a high-fat, high-cholesterol diet. Hepatic cholesterol and phospholipid contents in Abca1 -/-mice were indistinguishable from those in Abca1 +/-and Abca1 +/+ mice on both diets. In spite of the absence of HDL, biliary secretion rates of cholesterol, bile salts, and phospholipid were unimpaired in Abca1 -/-mice. Neither the hepatic expression levels of genes controlling key steps in cholesterol metabolism nor the contribution of de novo synthesis to biliary cholesterol and bile salts were affected by Abca genotype. Finally, fecal excretion of neutral and acidic sterols was similar in all groups. We conclude that plasma HDL levels and ABCA1 activity do not control net cholesterol transport from the periphery via the liver into the bile, indicating that the importance of HDL in reverse cholesterol transport requires re-evaluation.

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Section: Introductionmentioning

confidence: 99%

“…Macrophages from Abca1 -/-mice were shown to be impaired in engulfment of apoptotic bodies (22,23). In addition, the knockout mice showed defects in platelet aggregation caused by a defect in exposing phosphatidylserine at the outer leaflet of the platelet plasma membrane (23).…”

Section: Introductionmentioning

confidence: 99%

Hepatobiliary cholesterol transport is not impaired in Abca1-null mice lacking HDL

Groen¹,

Bloks²,

Bandsma³

et al. 2001

J. Clin. Invest.

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“…However, some progress in elucidating them has been made in the nematode, Caenorhabditis elegans, and in human and murine systems. Recent studies showed several receptors and molecules as being important in the uptake of apoptotic cells in mammals (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). The complement system was found to have a role in opsonization and interaction with apoptotic cells (17,20) and in apoptosis (21).…”

mentioning

confidence: 99%

Accelerated Fas-Mediated Apoptosis of Monocytes and Maturing Macrophages from Patients with Systemic Lupus Erythematosus: Relevance to In Vitro Impairment of Interaction with iC3b-Opsonized Apoptotic Cells

Shoshan

Shapira

Toubi

et al. 2001

The Journal of Immunology

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Impaired handling of apoptotic cells has been suggested as an important factor in the development of systemic lupus erythematosus (SLE), and a role for complement in the removal of apoptotic cells was shown recently. We studied the in vitro function of macrophages from 40 patients with SLE and their matched controls in the removal of heterologous apoptotic cells opsonized by iC3b. Interaction index of apoptotic cells opsonized by iC3b was significantly lower in patients with SLE and averaged 71% ± 37 of that of healthy individuals (p < 0.002) and 69% ± 35 of patients with rheumatoid arthritis (p < 0.007). SLE patients had increased apoptosis of both freshly isolated monocytes (p < 0.001) and maturing macrophages (p < 0.04) that led to decreased density of monocyte-derived macrophages. Apoptosis was inhibited by adding soluble Fas receptor indicating Fas-mediated apoptosis. As demonstrated in both healthy controls and patients with SLE, decreased macrophage density by itself caused significant decreased uptake of apoptotic cells by the remaining macrophages. Maintaining normal density in SLE patients either by an increased initial density or by using soluble Fas restored the interaction capacity of the individual macrophages in the majority of patients. We concluded that impaired in vitro interaction of iC3b-opsonized apoptotic cells with macrophages from patients with SLE was mainly associated with Fas-dependent accelerated apoptosis of the monocytes/macrophages. Accelerated apoptosis of phagocytes may represent a novel in vitro mechanism of impairment of interaction with apoptotic cells that, apart from reducing the number of professional phagocytes, alters the function of the remaining macrophages.

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“…10 A similar situation might exist in mammals; ABC1, the CED-7 homolog, acts in macrophages to remove necrotic cells as well as programmed cell deaths. 34 Other studies, however, have suggested that the receptors recognizing apoptotic cells and necrotic cells are distinct. 35 Clearly, more work on this issue is warranted.…”

Section: Recognition Of Dying Cellsmentioning

confidence: 99%

How the worm removes corpses: the nematode C. elegans as a model system to study engulfment

Gumienny

Hengartner

2001

Cell Death Differ

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Apoptotic cell death in the nematode C. elegans culminates with the removal of the dying cells from the organism. This removal is brought forth through a rapid and specific engulfment of the doomed cell by one of its neighbors. Over half a dozen genes have been identified that function in this process in the worm. Many of these engulfment genes have functional homologs in Drosophila and higher vertebrates. Indeed, there is growing evidence supporting the hypothesis that the pathways that mediate the removal of apoptotic cells might be, at least in part, conserved through evolution. Cell Death and Differentiation (2001) 8, 564 ± 568.

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ABCI, the mammalian homologue of the engulfment gene ced-7, is required during phagocytosis of both necrotic and apoptotic cells

Cited by 29 publications

References 14 publications

Hepatobiliary cholesterol transport is not impaired in Abca1-null mice lacking HDL

Hepatobiliary cholesterol transport is not impaired in Abca1-null mice lacking HDL

Accelerated Fas-Mediated Apoptosis of Monocytes and Maturing Macrophages from Patients with Systemic Lupus Erythematosus: Relevance to In Vitro Impairment of Interaction with iC3b-Opsonized Apoptotic Cells

How the worm removes corpses: the nematode C. elegans as a model system to study engulfment

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