Abdominal magnetic resonance imaging in small rodents using a clinical 1.5T MR scanner

Inderbitzin, Daniel; Stoupis, Christoforos; Sidler, Daniel; Gass, Markus; Candinas, Daniel

doi:10.1016/j.ymeth.2007.03.010

Cited by 16 publications

(8 citation statements)

References 39 publications

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“…genes that met the following criteria: after resection LCB ≥ 1.5, or before resection LCB ≥ 1.5, or A20 LCB ≥ 1.5 and βgal LCB not ≥ 1.5. We assessed GO enrichment of the A20 interacting genes by using BiNGO, a Cytoscape plugin which maps significantly enriched GO terms onto a visual representation of the GO hierarchy [12], [26], [27]. Our results demonstrated a significant enrichment (Benjamini-Hochberg adjusted P-value < 0.05) in all 3 categories of GO terms: cellular component, molecular function, and biological process (Fig.…”

Section: Resultsmentioning

confidence: 94%

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A20 Modulates Lipid Metabolism and Energy Production to Promote Liver Regeneration

Damrauer

Studer²,

Silva³

et al. 2011

PLoS ONE

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BackgroundLiver Regeneration is clinically of major importance in the setting of liver injury, resection or transplantation. We have demonstrated that the NF-κB inhibitory protein A20 significantly improves recovery of liver function and mass following extended liver resection (LR) in mice. In this study, we explored the Systems Biology modulated by A20 following extended LR in mice.Methodology and Principal FindingsWe performed transcriptional profiling using Affymetrix-Mouse 430.2 arrays on liver mRNA retrieved from recombinant adenovirus A20 (rAd.A20) and rAd.βgalactosidase treated livers, before and 24 hours after 78% LR. A20 overexpression impacted 1595 genes that were enriched for biological processes related to inflammatory and immune responses, cellular proliferation, energy production, oxidoreductase activity, and lipid and fatty acid metabolism. These pathways were modulated by A20 in a manner that favored decreased inflammation, heightened proliferation, and optimized metabolic control and energy production. Promoter analysis identified several transcriptional factors that implemented the effects of A20, including NF-κB, CEBPA, OCT-1, OCT-4 and EGR1. Interactive scale-free network analysis captured the key genes that delivered the specific functions of A20. Most of these genes were affected at basal level and after resection. We validated a number of A20's target genes by real-time PCR, including p21, the mitochondrial solute carriers SLC25a10 and SLC25a13, and the fatty acid metabolism regulator, peroxisome proliferator activated receptor alpha. This resulted in greater energy production in A20-expressing livers following LR, as demonstrated by increased enzymatic activity of cytochrome c oxidase, or mitochondrial complex IV.ConclusionThis Systems Biology-based analysis unravels novel mechanisms supporting the pro-regenerative function of A20 in the liver, by optimizing energy production through improved lipid/fatty acid metabolism, and down-regulated inflammation. These findings support pursuit of A20-based therapies to improve patients’ outcomes in the context of extreme liver injury and extensive LR for tumor treatment or donation.

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Section: Resultsmentioning

confidence: 94%

“…In order to identify over-represented gene ontology categories from the informative set of genes, we used the Cytoscape plugin BiNGO [12], [26], [27]. BiNGO maps the functions impacted by a given gene set on the GO hierarchy and outputs this mapping as a Cytoscape graph that assists in understanding biological theme.…”

Section: Methodsmentioning

confidence: 99%

A20 Modulates Lipid Metabolism and Energy Production to Promote Liver Regeneration

Damrauer

Studer²,

Silva³

et al. 2011

PLoS ONE

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“…The transformation of physiological signals from a mouse to be adequate for human scanners is a crucial point in terms of amplitude, frequency as well as vendor specifications. Therefore, respiratory gating of mice on human MR scanners remains challenging [46]. The ability to detect co-labeled MSC up to the single cell level in-vivo appears interesting to investigate homing and local engraftment of these cells because after systemic administration only a small percentage of transplanted MSC can be detected in the bone marrow and different organs [47].…”

Section: Discussionmentioning

confidence: 99%

Magnetic Resonance Imaging of Single Co-Labeled Mesenchymal Stromal Cells after Intracardial Injection in Mice

Salamon

Wicklein

Didié

et al. 2014

Fortschr Röntgenstr

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Ziel: Etablierung einer Doppelmarkierung mesenchymaler Stromazellen (MSZ) zur in-vivo Detektion einzelner MSZ mittels MRT und zur histologischen Validierung. Material und Methoden: Murine MSZ wurden mit fluoreszierenden Eisenmikropartikeln und Carboxyfluorescein Succinimidyl Ester (CFSE) markiert. Der zelluläre Eisengehalt wurde mittels Atomabsorptionsspektrometrie bestimmt. Zellprolieferation und Expression charakteristischer Oberflächenmarker wurden mittels Durchflusszytometrie bestimmt. Die chondrogene Differenzierungskapazität wurde überprüft. Verschiedene Zellanzahlen (n1 = 5000, n2 = 15 000, n3 = 50 000) wurden bei 12 Mäusen in den linken Herzventrikel injiziert. Es erfolgte die sequenzielle MRT der Tiere an einem klinischen 3.0 T MRT. Zur histologischen Validierung wurden Kryostatschnitte fluoreszensmikroskopisch untersucht. Ergebnisse: Die magnetische und fluoreszierende Doppelmarkierung von MSZ wurde etabliert (mittlerer zellulärer Eisengehalt 23,6 ± 4,3 pg). Durchflusszytometrisch zeigten sich ähn-liche Zellprolieferationsraten und Rezeptorexpressionsprofile von markierten und unmarkierten MSZ. Die chondrogene Differenzierung der doppelt markierten MSZ wurde verifiziert. Nach Zellinjektion zeigten sich im MRT multiple Signalauslöschungen im Hirn und geringer in der Niere. Im Hirn fanden sich durchschnittlich 4,6 ± 1,2 (n1), 9,0 ± 3,6 (n2) und 25,0 ± 1,0 (n3) Signalauslöschungen pro Schicht. Durchschnittlich fanden sich 8,7 ± 3,1 (n1), 22,0 ± 6,1 (n2) und 89,8 ± 6,5 (n3) Zellen pro korrespondierendem Kryostatschnitt. Die statistische Korrelation der mittels MRT detektierten Signalauslöschungen und der histologisch nachgewiesenen Zellen ergab einen Korrelationskoeffizienten von r = 0,91. Schlussfolgerung: Die Studie zeigt die erfolgreiche magnetische und fluoreszierende DoppelmarkieAbstract ! Purpose: The aim of this study was to establish co-labeling of mesenchymal stromal cells (MSC) for the detection of single MSC in-vivo by MRI and histological validation. Materials and Methods: Mouse MSC were co-labeled with fluorescent iron oxide micro-particles and carboxyfluorescein succinimidyl ester (CFSE). The cellular iron content was determined by atomic absorption spectrometry. Cell proliferation and expression of characteristic surface markers were determined by flow cytometry. The chondrogenic differentiation capacity was assessed. Different amounts of cells (n1 = 5000, n2 = 15 000, n3 = 50 000) were injected into the left heart ventricle of 12 mice. The animals underwent sequential MRI on a clinical 3.0 T scanner (Intera, Philips Medical Systems, Best, The Netherlands). For histological validation cryosections were examined by fluorescent microscopy. Results: Magnetic and fluorescent labeling of MSC was established (mean cellular iron content 23.6 ± 3 pg). Flow cytometry showed similar cell proliferation and receptor expression of labeled and unlabeled MSC. Chondrogenic differentiation of labeled MSC was verified. After cell injection MRI revealed multiple signal voids in the brain and fewer signal voids...

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“…T 2 ‐weighted imaging has proven to be a good mechanism for abdominal imaging due to the excellent soft‐tissue contrast that can be obtained. It is routinely used for detection and characterization of liver lesion clinically (11–13), as well as in small animals (14, 15). Clinical studies focusing on the abdominal region rely on breath‐hold imaging to mitigate the effects of respiratory motion (16, 17).…”

Section: Discussionmentioning

confidence: 99%

Multishot PROPELLER for high‐field preclinical MRI

Pandit

Story³

et al. 2010

Magnetic Resonance in Med

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With the development of numerous mouse models of cancer, there is a tremendous need for an appropriate imaging technique to study the disease evolution. High-field T2-weighted imaging using PROPELLER MRI meets this need. The 2-shot PROPELLER technique presented here, provides (a) high spatial resolution, (b) high contrast resolution, and (c) rapid and non-invasive imaging, which enables high-throughput, longitudinal studies in free-breathing mice. Unique data collection and reconstruction makes this method robust against motion artifacts. The 2-shot modification introduced here, retains more high-frequency information and provides higher SNR than conventional single-shot PROPELLER, making this sequence feasible at high-fields, where signal loss is rapid. Results are shown in a liver metastases model to demonstrate the utility of this technique in one of the more challenging regions of the mouse, which is the abdomen.

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Abdominal magnetic resonance imaging in small rodents using a clinical 1.5T MR scanner

Cited by 16 publications

References 39 publications

A20 Modulates Lipid Metabolism and Energy Production to Promote Liver Regeneration

A20 Modulates Lipid Metabolism and Energy Production to Promote Liver Regeneration

Magnetic Resonance Imaging of Single Co-Labeled Mesenchymal Stromal Cells after Intracardial Injection in Mice

Multishot PROPELLER for high‐field preclinical MRI

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