Abdominal subcutaneous and omental adipocyte morphology and its relation to gene expression, lipolysis and adipocytokine levels in women

Michaud, Andréanne; Boulet, Marie Michèle; Veilleux, Alain; Noël, Suzanne; Paris, Gaétan; Tchernof, André

doi:10.1016/j.metabol.2013.11.007

Cited by 44 publications

(35 citation statements)

References 39 publications

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“…A few studies also reported that fat cell size seems to be a marker of altered adipocyte lipid metabolism (3,8,14,15,19). Previous studies reported that large adipocytes were characterized by higher rates of glycerol release compared to smaller adipocytes from the same fat compartment (15,38).…”

Section: Discussionmentioning

confidence: 99%

“…Lipid weight of the cell suspension was measured using Dole's extraction. Data were expressed in mmol/10 6 cells per 2 h (12,14).…”

Section: Adipocyte Isolation and Adipocyte Lipolysis Measurementmentioning

confidence: 99%

“…Many previous studies (5,6,14,15,16,17,18,19) and one recent review (8) reported that increased adipocyte size, in either the subcutaneous (SC) or visceral fat compartment, was associated with metabolic alterations and markers of adipose tissue dysfunction, independent of overall adiposity. However, in most of these studies, detailed variations in fat cell size populations were not considered (8).…”

Section: Introductionmentioning

confidence: 99%

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Abdominal adipocyte populations in women with visceral obesity

Michaud¹,

Laforest²,

Pelletier³

et al. 2016

European Journal of Endocrinology

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Background: Visceral obesity is independently related to numerous cardiometabolic alterations, with adipose tissue dysfunction as a central feature. Objective: To examine whether omental (OM) and subcutaneous (SC) adipocyte size populations in women relate to visceral obesity, cardiometabolic risk factors and adipocyte lipolysis independent of total adiposity. Design and methods: OM and SC fat samples were obtained during gynecological surgery in 60 women (mean age, 46.1G5.9 years; mean BMI, 27.1G4.5 kg/m 2 (range, 20.3-41.1 kg/m 2 )). Fresh samples were treated with osmium tetroxide and were

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Section: Discussionmentioning

confidence: 99%

“…Lipid weight of the cell suspension was measured using Dole's extraction. Data were expressed in mmol/10 6 cells per 2 h (12,14).…”

Section: Adipocyte Isolation and Adipocyte Lipolysis Measurementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Abdominal adipocyte populations in women with visceral obesity

Michaud¹,

Laforest²,

Pelletier³

et al. 2016

European Journal of Endocrinology

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“…Adipocyte weight and cell number in the suspensions were calculated using lipid weight, average cell volume and the density of triolein as previously described. 5 Lipid profile and glucose homeostasis Blood samples were obtained on the morning of surgery after a 12-hour fast. Cholesterol and triglyceride levels were measured in plasma and lipoprotein fractions with a Technicon RA-500 analyzer (Bayer, Etobicoke, ON, Canada) using enzymatic methods or with the Olympus AU400 (Beckman Coulter, Mississauga, Canada).…”

Section: Body Fatness and Body Fat Distribution Measurementsmentioning

confidence: 99%

“…4 We also found that visceral adipocyte hypertrophy is related to alterations in lipolysis and adipose tissue expression of genes coding for proteins involved in adipocyte metabolism or inflammation, independent of overall adiposity and body fat distribution. 5 Further, obesity is associated with extracellular matrix remodelling that often leads to the development of fibrosis in adipose tissue. 6,7 These alterations may partially explain the increased cardiometabolic risk associated with the visceral obesity phenotype.…”

Section: Introductionmentioning

confidence: 99%

Computed tomography-measured adipose tissue attenuation and area both predict adipocyte size and cardiometabolic risk in women

et al. 2015

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Objective: To assess the ability of CT-derived measurements including adipose tissue attenuation and area to predict fat cell hypertrophy and related cardiometabolic risk. Methods: Abdominal adipose tissue areas and radiologic attenuation were assessed using 4 CT images in 241 women (age: 47 years, BMI: 26.5 kg/m 2 ). Fat cell weight was measured in paired VAT and SAT samples. Fasting plasma lipids, glucose and insulin levels were measured. Results: Adipose tissue attenuation was negatively correlated with SAT (rD-0.46) and VAT (rD-0.67) fat cell weight in the corresponding depot (p<0.0001 for both). Women with visceral adipocyte hypertrophy had higher total-, VLDL-, LDL-and HDL-triglyceride and apoB levels as well as a higher cholesterol/HDL-cholesterol ratio, fasting glucose and insulin levels compared to women with smaller visceral adipocytes. Adjustment for VAT area minimized these differences while subsequent adjustment for attenuation eliminated all differences, with the exception of fasting glycaemia. In SAT, adjustment for VAT area and attenuation eliminated all adipocyte hypertrophy-related alterations except for fasting hyperglycaemia. Conclusion: CT-derived adipose tissue attenuation and area both contribute to explain variation in the cardiometabolic risk profile associated with the same biological parameter: visceral fat cell hypertrophy.

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Therapeutic targeting of white adipose tissue metabolic dysfunction in obesity: mechanisms and opportunities

Yang,

Chen,

Zhang

et al. 2024

MedComm

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White adipose tissue is not only a highly heterogeneous organ containing various cells, such as adipocytes, adipose stem and progenitor cells, and immune cells, but also an endocrine organ that is highly important for regulating metabolic and immune homeostasis. In individuals with obesity, dynamic cellular changes in adipose tissue result in phenotypic switching and adipose tissue dysfunction, including pathological expansion, WAT fibrosis, immune cell infiltration, endoplasmic reticulum stress, and ectopic lipid accumulation, ultimately leading to chronic low‐grade inflammation and insulin resistance. Recently, many distinct subpopulations of adipose tissue have been identified, providing new insights into the potential mechanisms of adipose dysfunction in individuals with obesity. Therefore, targeting white adipose tissue as a therapeutic agent for treating obesity and obesity‐related metabolic diseases is of great scientific interest. Here, we provide an overview of white adipose tissue remodeling in individuals with obesity including cellular changes and discuss the underlying regulatory mechanisms of white adipose tissue metabolic dysfunction. Currently, various studies have uncovered promising targets and strategies for obesity treatment. We also outline the potential therapeutic signaling pathways of targeting adipose tissue and summarize existing therapeutic strategies for antiobesity treatment including pharmacological approaches, lifestyle interventions, and novel therapies.

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Abdominal subcutaneous and omental adipocyte morphology and its relation to gene expression, lipolysis and adipocytokine levels in women

Cited by 44 publications

References 39 publications

Abdominal adipocyte populations in women with visceral obesity

Abdominal adipocyte populations in women with visceral obesity

Computed tomography-measured adipose tissue attenuation and area both predict adipocyte size and cardiometabolic risk in women

Therapeutic targeting of white adipose tissue metabolic dysfunction in obesity: mechanisms and opportunities

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