Aberrant DNA methylation patterns of human spermatozoa in current smoker males

Laqqan, Mohammed M.; Tierling, Sascha; Alkhaled, Y.; Porto, Christina Lo; Solomayer, Erich‐Franz; Hammadeh, Mohamad Eid

doi:10.1016/j.reprotox.2017.05.010

Cited by 36 publications

(20 citation statements)

References 68 publications

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“…Some of the identified CpG sites and/or affected genes for cigarette smokers are in common with those identified here for cannabis use. Laggan et al [32]. used the Illumina HumanMethylation450 beadchip and identified differentially methylated sites in the MAPK8IP3 and TKR genes.…”

Section: Discussionmentioning

confidence: 99%

Cannabinoid exposure and altered DNA methylation in rat and human sperm

et al. 2018

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Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol (THC) exposure and altered DNA methylation in sperm from humans and rats, respectively. DNA methylation, measured by reduced representation bisulfite sequencing, differed in the sperm of human users from non-users by at least 10% at 3,979 CpG sites. Pathway analyses indicated Hippo Signaling and Pathways in Cancer as enriched with altered genes (Bonferroni p < 0.02). These same two pathways were also enriched with genes having altered methylation in sperm from THC-exposed versus vehicleexposed rats (p < 0.01). Data validity is supported by significant correlations between THC exposure levels in humans and methylation for 177 genes, and substantial overlap in THC target genes in rat sperm (this study) and genes previously reported as having altered methylation in the brain of rat offspring born to parents both exposed to THC during adolescence. In humans, cannabis use was also associated with significantly lower sperm concentration. Findings point to possible pre-conception paternal reproductive risks associated with cannabis use.

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Section: Discussionmentioning

confidence: 99%

Cannabinoid exposure and altered DNA methylation in rat and human sperm

et al. 2018

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“…Significantly different methylation level (>20%) in 11 CpG dinucleotides has been shown in spermatozoa of current smokers compared with nonsmokers. Two of 11 CpGs (cg19169023 and cg07869343) have been found to be located in the spermatogenesis‐related tyrosine‐protein kinase receptor ( TKR ) and mitogen‐activated protein kinase 8 interacting protein 3 ( MAPK8IP3) genes, and shown strong correlations with various semen parameters such as sperm count and motility, proposing that smoking has an adverse effect on spermatogenesis via methylation changes in the sperm DNA (Laqqan et al, ).…”

Section: Cigarette Smoking and Epigenetic Alterationsmentioning

confidence: 99%

“…Cigarette smoking and male infertility have been evaluated from various aspects including semen parameters (Asare‐Anane et al, ; Vine, Tse, Hu, & Truong, ), sperm membrane integrity (Belcheva, Ivanova‐Kicheva, Tzvetkova, & Marinov, ), oxidative stress (OS) (Fraga, Motchnik, Wyrobek, Rempel, & Ames, ; Saleh, Agarwal, Sharma, Nelson, & Thomas, ) and genetic and epigenetic effects (De Bantel et al, ; Jurewicz et al, ; Laqqan et al, ; Marczylo, Amoako, Konje, Gant, & Marczylo, ). Conventional semen parameters and smoking have been found to be associated with many studies (Caserta et al, ; Meri, Irshid, Migdadi, Irshid, & Mhanna, ; Vine, Margolin, Morrison, & Hulka, ).…”

Section: Introductionmentioning

confidence: 99%

Smoking-induced genetic and epigenetic alterations in infertile men

et al. 2018

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Male fertility rates have shown a progressive decrease in both developing and industrialised countries in the past 50 years. Clinical and epidemiological studies have demonstrated controversial results about the harmful effects of cigarette smoking on seminal parameters. Some studies could not establish a negative effect by tobacco smoking on sperm quality and function, whereas others have found a significant reduction in sperm quality and function. This study reviews the components in cigarette smoke and discusses the effects of smoking on male fertility by focusing extensively on smoking-induced genetic and epigenetic alterations in infertile men. Chromosomal aneuploidies, sperm DNA fragmentation and gene mutations are discussed in the first section, while changes in DNA methylation, chromatin remodelling and noncoding RNAs are discussed in the second section as part of epigenetic alterations.

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“…Kobayashi et al showed that exercise and living environment are associated with imprint methylation (Kobayashi et al, ). Smoking is also associated with sperm DNA methylation patterns (Dong et al, ; Laqqan et al, ). In addition, alcohol consumption has a strong correlation with DNA methylation of imprinted gene H19 in humans and mice spermatozoa (Lim & Song, ; Ouko et al, ; Stouder, Somm, & Paoloni‐Giacobino, ).…”

Section: Discussionmentioning

confidence: 99%

Methylation levels ofIGF2andKCNQ1in spermatozoa from infertile men are associated with sperm DNA damage

Pan

et al. 2019

Andrologia

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Abnormal imprinted genes methylation in spermatozoa has been shown to be associated with subfertility. However, the relationship between sperm DNA damage and specific imprinted genes methylation remains unclear. In this study, DNA methylation levels were determined at seven imprinted genes loci (H19, INS‐IGF2, KCNQ1, MEG3, MEST, PEG3 and SNRPN) in 66 semen samples using the MSRE‐qPCR method. The semen samples were divided into two groups according to the threshold value (25%) of DNA fragmentation index (DFI). We found that the mean methylation level at IGF2 (cg17037101) in the group with DFI ≥ 25% was lower than that in the group with DFI < 25% (13.7 ± 3% vs. 31.5 ± 5.3%, p = 0.0053). However, the methylation levels of other CpGs did not differ from the imprinted genes. Correlation analysis of DFI with the methylation levels of imprinted genes demonstrated that the IGF2 (cg17037101) methylation level was negatively correlated with sperm DFI (r = −0.448, p = 0.0038), and the KCNQ1 (cg24932449) methylation level was positively correlated with sperm DFI (r = 0.354, p = 0.0273). Our results suggest that the aberrant methylation of IGF2 and KCNQ1 genes may be associated with sperm DNA damage.

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Aberrant DNA methylation patterns of human spermatozoa in current smoker males

Cited by 36 publications

References 68 publications

Cannabinoid exposure and altered DNA methylation in rat and human sperm

Cannabinoid exposure and altered DNA methylation in rat and human sperm

Smoking-induced genetic and epigenetic alterations in infertile men

Methylation levels ofIGF2andKCNQ1in spermatozoa from infertile men are associated with sperm DNA damage

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