Aberrant gene expression of superoxide dismutases in Chlamydia trachomatis-infected recurrent spontaneous aborters

Abstract

Study aimed to characterize the expression of antioxidant genes SOD1 and SOD2 in Chlamydia trachomatis-induced recurrent spontaneous aborters and further determine their role by in silico analysis. First void urine was collected from 130 non-pregnant women with history of recurrent spontaneous abortion (RSA) (Group I) and 130 non-pregnant women (Group II; control) attending Obstetrics and Gynecology Department, SJH, New Delhi, India. C. trachomatis detection was performed by conventional PCR in urine. Gene expression of SOD1 and SOD2 was performed by quantitative real-time PCR. Further, its interacting partners were studied by in silico analysis. 22 patients were positive for C. trachomatis in Group I. Significant upregulation was observed for SOD2 gene in C. trachomatis-infected RSA patients while SOD1 was found to be downregulated. Increased concentration of oxidative stress biomarkers 8-hydroxyguanosine and 8-isoprostane was found in C. trachomatis-infected RSA patients. Protein–protein interaction (PPI) of SOD proteins and its interacting partners viz.; CCS, GPX1, GPX2, GPX3, GPX4, GPX5, GPX7, GPX8, CAT, PRDX1, TXN, SIRT3, FOXO3, and AKT1 were found to be involved in MAPK, p53 and foxo signaling pathways. Molecular pathways involved in association with SODs indicate reactive oxygen species (ROS) detoxification, apoptotic pathways and cell cycle regulation. Overall data revealed alleviated levels of SOD2 gene and decreased expression of SOD1 gene in response to C. trachomatis-infection leading to production of oxidative stress and RSA.

Role of increased IGFBP2 in trophoblast cell proliferation and recurrent spontaneous abortion development: A pilot study

“…Reactive oxygen species (ROS) are closely associated with a number of physiological and pathological functions and may influence the function of trophoblast and outcome of pregnancy (Dos Anjos Cordeiro et al, 2023;Ye et al, 2023). More recently, Ray et al reported that the single nucleotide polymorphisms and expression level of SOD1 and SOD2 are associated with C. trachomatisinfected recurrent spontaneous abortion RSA (Ray et al, 2022(Ray et al, , 2023. These studies indicate that SOD1 may be an important potential factor related to RSA.…”

“…More recently, Ray et al. reported that the single nucleotide polymorphisms and expression level of SOD1 and SOD2 are associated with C. trachomatis‐infected recurrent spontaneous abortion RSA (Ray et al., 2022 , 2023 ). These studies indicate that SOD1 may be an important potential factor related to RSA.…”

MicroRNA mediated regulation of oxidative stress and cytokines in Chlamydia trachomatis‐infected recurrent spontaneous abortion: A case‐control study

“…RNA isolation was carried out using Trizol method (Invitrogen, USA) from urine as described earlier 27 . qRT‐PCR analysis was performed in C. trachomatis ‐positive samples ( n = 06), C. trachomatis ‐negative samples ( n = 24) and controls ( n = 30).…”

“…Quantitative expression analysis of SOD and cytokine genes RNA isolation was carried out using Trizol method (Invitrogen, USA) from urine as described earlier. 27 2.…”

Determination of chlamydial load in recurrent miscarriage in relation to some female sex hormones: a case-control study

“…C. trachomatis is an intracellular sexually transmitted pathogen with a biphasic life cycle consisting of the elementary body as the infectious form and reticulate body as the metabolically active noninfectious form [2]. The association of C. trachomatis and pregnancy complications including RSA has been reported in serum, endometrial tissue and urine in several studies but the mechanistic approach utilized by the pathogen to induce abortion is still under active investigation [3][4][5][6]. C. trachomatis targets the columnar epithelial cells of the female genital tract.…”