Aberrant innate immune responses in TLR-ligand activated HLA-B27 transgenic rat cells

Qian, Bi Feng; Tonkonogy, Susan L.; Sartor, R. Balfour

doi:10.1002/ibd.20502

Cited by 15 publications

(8 citation statements)

References 57 publications

(48 reference statements)

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“…An incidental but consistent finding was that, when compared with age-matched controls, the splenocytes from B27-TG rats had consistently higher proportions of granulocytes, the majority of which were CD11b. An enhanced granulocytic infiltrate has been observed macroscopically in the gastrointestinal (GI) tract of B27-TG rats,23 while a second study has reported that B27-TG rat spleens contain a higher proportion of CD11b cells compared with control littermates 24. It is unclear whether CD11b granulocytes merely represent ongoing inflammation or play a pathogenic role, perhaps through an expression of innate immune receptors, such as PIR, which can interact with NC-B27.…”

Section: Discussionmentioning

confidence: 99%

Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply

et al. 2013

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Section: Discussionmentioning

confidence: 99%

Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply

et al. 2013

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“…It has been shown in several systems, including B27/Hu␤ 2 m-transgenic rats, that B27 heavy chains have an unusual tendency to misfold and form homodimers and higher oligomers (21,(34)(35)(36). This misfolding can induce an unfolded protein response that can synergize with innate immune signals in the activation of a proinflammatory cytokine response (37), and other aberrant innate responses have also been documented (38). Our findings are also consistent with data from human and murine systems suggesting recognition of HLA-B27 by CD4ϩ T cells (39,40).…”

Section: Discussionmentioning

confidence: 99%

Spondylarthritis in HLA–B27/human β₂‐microglobulin–transgenic rats is not prevented by lack of CD8

Taurog¹,

Dorris²,

Satumtira³

et al. 2009

Arthritis & Rheumatism

126

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Objective. HLA-B27 predisposes to spondylarthritis by an unknown mechanism. A logical candidate mechanism is through recognition of B27 by CD8؉ T cells. The purpose of this study was to examine the effects of a lack of CD8 on the spondylarthritis that develops in B27/human ␤ 2 -microglobulin (Hu␤ 2 m)-transgenic rats.Methods. A missense mutation in the CD8a gene that causes a loss of CD8␣ expression was identified in offspring of a male Sprague-Dawley rat that had been treated with the mutagen N-ethyl-N-nitrosourea. The mutation was crossed into B27/Hu␤ 2 m-transgenic lines on the Lewis background. CD8a -/-and CD8a ؉/-progeny were compared on a mixed SD-LEW background as well as after at least 10 backcrosses to LEW rats. CD8 function was assessed by generating cytolytic T lymphocytes (CTLs) against allogeneic DA strain antigens.Results. Homozygous mutant rats showed normal CD8a and CD8b messenger RNA levels but no detectable expression of either protein and an almost complete abrogation of the allogeneic CTL response. Two disease phenotypes previously observed in different B27/ Hu␤ 2 m-transgenic lines also occurred in the respective CD8a -/--transgenic rat lines. There was no significant difference in disease prevalence or severity between CD8a -/-rats and CD8a ؉/-rats. Conclusion. All of the previously described disease manifestations in HLA-B27/Hu␤ 2 m-transgenic rats arise in the absence of any functional CD8؉ T cells. It thus seems unlikely that classic T cell recognition of HLA-B27 is of primary importance in this animal model. The possibility of a secondary role of a CD8-dependent mechanism cannot be entirely excluded.

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“…It has been shown in several systems, including B27/Huβ 2 m‐transgenic rats, that B27 heavy chains have an unusual tendency to misfold and form homodimers and higher oligomers (21, 34–36). This misfolding can induce an unfolded protein response that can synergize with innate immune signals in the activation of a proinflammatory cytokine response (37), and other aberrant innate responses have also been documented (38). Our findings are also consistent with data from human and murine systems suggesting recognition of HLA–B27 by CD4+ T cells (39, 40).…”

Section: Discussionmentioning

confidence: 99%

Improving dispersion of multiwalled carbon nanotubes in polyamide 6 composites through amino‐functionalization

Fang

Tong

et al. 2007

J of Applied Polymer Sci

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The focus of this study is to investigate the state of dispersion of different treated multiwalled carbon nanotubes (MWNTs) in polyamide 6 (PA6). The MWNTs used in composites were grafted by 1,6-hexamethylenediamine (HMD) via acid-thionyl chloride to improve their compatibility with PA6 matrix. A microstructure transformation of MWNTs is found during the treatment process. Acidification makes the MWNTs compact and grafting HMD promotes the compact structure loose again. The MWNTs after different treatment were used to fabricate MWNTs/PA6 composites through melt blending. The dispersion of different MWNTs in PA6 was observed by a combination of scanning electron microscopy, optical microscopy, and transmission electron microscopy. The results show that the amino-functionalized MWNTs are dispersed more homogeneously in PA6 than the purified MWNTs, and the poorest dispersion is achieved for acid treated MWNTs. It is indicated that the loose structure and functionalized surface of MWNTs benefit the dispersion of MWNTs in PA6. In addition, the aminofunctionalization of MWNTs improves the compatibility between the MWNTs and PA6, resulting in stronger interfacial adhesion.

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Aberrant innate immune responses in TLR-ligand activated HLA-B27 transgenic rat cells

Cited by 15 publications

References 57 publications

Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply

Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply

Spondylarthritis in HLA–B27/human β₂‐microglobulin–transgenic rats is not prevented by lack of CD8

Improving dispersion of multiwalled carbon nanotubes in polyamide 6 composites through amino‐functionalization

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Aberrant innate immune responses in TLR-ligand activated HLA-B27 transgenic rat cells

Cited by 15 publications

References 57 publications

Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply

Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply

Spondylarthritis in HLA–B27/human β2‐microglobulin–transgenic rats is not prevented by lack of CD8

Improving dispersion of multiwalled carbon nanotubes in polyamide 6 composites through amino‐functionalization

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Spondylarthritis in HLA–B27/human β₂‐microglobulin–transgenic rats is not prevented by lack of CD8