Aberrant Production of Th1/Th2/Th17-Related Cytokines  in Serum of C57BL/6 Mice after Short-Term Formaldehyde Exposure

Wei, Haiyan; Tan, Kehong; Sun, Rongli; Yin, Lu; Zhang, Juan; Pu, Yuepu

doi:10.3390/ijerph111010036

Cited by 20 publications

(25 citation statements)

References 53 publications

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“…Briefly, LNCs obtained from mice immunized with PLP 139-151 were stimulated with PLP 139-151 or control (NASE 101-120) with or without DHT or its ethanol. First, we analyzed the cytokine secretion in culture supernatants on day 3 poststimulation using cytokine capture beads to include a panel of Th1, Th2, and Th17 cytokines, in addition to two other inflammatory cytokines, IL-6 and TNF-α (Wei et al, 2014). The data revealed that supernatants obtained from cells stimulated with PLP 139-151 with or without ethanol showed the presence of all the cytokines, in the order from Th1, followed by Th17 and Th2 cytokines, and TNF-α and IL-6 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Association of Autophagy in the Cell Death Mediated by Dihydrotestosterone in Autoreactive T Cells Independent of Antigenic Stimulation

Jia

Anandhan

Massilamany

et al. 2015

J Neuroimmune Pharmacol

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Gender disparity is well documented in the mouse model of experimental autoimmune encephalomyelitis (EAE) induced with proteolipid protein (PLP) 139-151, in which female, but not male, SJL mice show a chronic relapsing-remitting paralysis. Furthermore, dihydrotestosterone (DHT) has been shown to ameliorate the severity of EAE, but the underlying mechanisms of its protective effects are unclear. Using major histocompatibility complex (MHC) class II dextramers for PLP 139-151, we tested the hypothesis that DHT selectively modulates the expansion and functionalities of antigen-specific T cells. Unexpectedly, we noted that DHT induced cell death in antigen-specific, autoreactive T cells, but the effects were not selective, because both proliferating and non-proliferating cells were equally affected independent of antigenic stimulation. Furthermore, DHT-exposed PLP 139-151-specific T cells did not show any shift in cytokine production; rather, frequencies of cytokine-producing PLP-specific T cells were significantly reduced, irrespective of T helper (Th) 1, Th2, and Th17 subsets of cytokines. By evaluating cell death and autophagy pathways, we provide evidence for the induction of autophagy to be associated with cell death caused by DHT. Taken together, the data provide new insights into the role of DHT and indicate that cell death and autophagy contribute to the therapeutic effects of androgens in autoreactive T cells.

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Section: Resultsmentioning

confidence: 99%

Association of Autophagy in the Cell Death Mediated by Dihydrotestosterone in Autoreactive T Cells Independent of Antigenic Stimulation

Jia

Anandhan

Massilamany

et al. 2015

J Neuroimmune Pharmacol

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“…IL-2 and IFN-γ are produced by Th1 cells to promote viral clearance by stimulating proliferation and cytotoxic T lymphocyte responses [ 45 ]. IL-4 is involved in a Th2 response, and IL-10 produces an anti-inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of protective efficacy of three novel H3N2 canine influenza vaccines

Zhou

et al. 2017

Oncotarget

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Canine influenza virus (CIV) has the potential risk to spread in different areas and dog types. Thus, there is a growing need to develop an effective vaccine to control CIV disease. Here, we developed three vaccine candidates: 1) a recombinant pVAX1 vector expressing H3N2 CIV hemagglutinin (pVAX1-HA); 2) a live attenuated canine adenovirus type 2 expressing H3N2 CIV hemagglutinin (rCAV2-HA); and 3) an inactivated H3N2 CIV (A/canine/Guangdong/01/2006 (H3N2)). Mice received an initial intramuscular immunization that followed two booster injections at 2 and 4 weeks post-vaccination (wpv). The splenic lymphocytes were collected to assess the immune responses at 6 wpv. The protective efficacy was evaluated by challenging H3N2 CIV after vaccination (at 6 wpv). Our results demonstrated that all three vaccine candidates elicited cytokine and antibody responses in mice. The rCAV2-HA vaccine and the inactivated vaccine generated efficient protective efficacy in mice, whereas limited protection was provided by the pVAX1-HA DNA vaccine. Therefore, both the rCAV2-HA live recombinant virus and the inactivated CIV could be used as potential novel vaccines against H3N2CIV. This study provides guidance for choosing the most appropriate vaccine for the prevention and control of CIV disease.

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“…Male C57BL/6 mice were housed as previously described [ 55 ]. Normal diets and water were given ad libitum throughout the study period.…”

Section: Methodsmentioning

confidence: 99%

Benzene-Induced Aberrant miRNA Expression Profile in Hematopoietic Progenitor Cells in C57BL/6 Mice

Wei

Zhang

Tan

et al. 2015

IJMS

Self Cite

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Benzene is a common environmental pollutant that causes hematological alterations. MicroRNAs (miRNAs) may play a role in benzene-induced hematotoxicity. In this study, C57BL/6 mice showed significant hematotoxicity after exposure to 150 mg/kg benzene for 4 weeks. Benzene exposure decreased not only the number of cells in peripheral blood but also hematopoietic progenitor cells in the bone marrow. Meanwhile, RNA from Lin− cells sorted from the bone marrow was applied to aberrant miRNA expression profile using Illumina sequencing. We found that 5 miRNAs were overexpressed and 45 miRNAs were downregulated in the benzene exposure group. Sequencing results were confirmed through qRT-PCR. Furthermore, we also identified five miRNAs which significantly altered in Lin−c-Kit+ cells obtained from benzene-exposed mice, including mmu-miR-34a-5p; mmu-miR-342-3p; mmu-miR-100-5p; mmu-miR-181a-5p; and mmu-miR-196b-5p. In summary, we successfully established a classical animal model to induce significant hematotoxicity by benzene injection. Benzene exposure may cause severe hematotoxicity not only to blood cells in peripheral circulation but also to hematopoietic cells in bone marrow. Benzene exposure also alters miRNA expression in hematopoietic progenitor cells. This study suggests that benzene induces alteration in hematopoiesis and hematopoiesis-associated miRNAs.

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Aberrant Production of Th1/Th2/Th17-Related Cytokines in Serum of C57BL/6 Mice after Short-Term Formaldehyde Exposure

Cited by 20 publications

References 53 publications

Association of Autophagy in the Cell Death Mediated by Dihydrotestosterone in Autoreactive T Cells Independent of Antigenic Stimulation

Association of Autophagy in the Cell Death Mediated by Dihydrotestosterone in Autoreactive T Cells Independent of Antigenic Stimulation

Evaluation of protective efficacy of three novel H3N2 canine influenza vaccines

Benzene-Induced Aberrant miRNA Expression Profile in Hematopoietic Progenitor Cells in C57BL/6 Mice

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