Aberrant Uterine Natural Killer (NK)‐Cell Expression and Altered Placental and Serum Levels of the NK‐Cell Promoting Cytokine Interleukin‐12 in Pre‐Eclampsia

Bachmayer, Nora; Hamad, Rangeen Rafik; Liszka, Lucie; Bremme, Katarina; Sverremark-Ekström, Eva

doi:10.1111/j.1600-0897.2006.00429.x

Cited by 99 publications

(87 citation statements)

References 35 publications

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“…However, the findings contrast with those of Stallmach et al (1999) and Bachmayer et al (2006) who reported increased numbers of CD56C cells in PE decidua compared with controls. In the absence of information on the FGR status of the PE study groups in these latter studies, use of placental bed biopsy and the avidinbiotin method, which is more sensitive than the PAP method used by Stallmach et al (1999), may also partly account for the differences in uNK cell numbers.…”

Section: Discussioncontrasting

confidence: 55%

Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy

Williams¹,

Bulmer²,

Searle³

et al. 2009

Reproduction

149

113

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Alterations in the balance of leucocyte populations in uterine decidua may lead to the generation of an unfavourable cytokine environment that is associated with unsuccessful pregnancy. Single and double immunohistochemical labelling was used to examine leucocyte populations in decidua from normal third trimester, foetal growth-restricted and pre-eclamptic pregnancies. Placental bed biopsies from 12 women undergoing elective Caesarean section with no hypertension or foetal growth restriction (FGR), 8 women with FGR without maternal hypertension and 12 women with pre-eclampsia (PE) were used to quantify decidual CD56C uterine NK cells, CD14C macrophages, CD3CT-lymphocytes and CD8C lymphocytes. CD3CCD56C, CD8CCD56C and CD161CCD3C double-labelled cells in decidua were compared in PE and control decidua. Decidual CD3CT-lymphocytes (P!0.01), CD8C cytotoxic T-lymphocytes (P!0.05), CD14C macrophages (P!0.0001) and CD56C uterine natural killer (uNK) cells (PZ0.01) were decreased in placental bed biopsies from women with PE compared with control third trimester decidua. By contrast, only CD56C uNK cells were decreased in FGR decidua (P!0.05). Double-positive CD8CCD56C cells were also decreased in PE compared with control third trimester decidua (P!0.05). The reduction in specific leucocyte subset numbers in PE and uNK cells in FGR suggests that altered local cytokine balance may be important in defective trophoblast invasion and spiral artery transformation in these pathological pregnancies.

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Section: Discussioncontrasting

confidence: 55%

Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy

Williams¹,

Bulmer²,

Searle³

et al. 2009

Reproduction

149

113

View full text Add to dashboard Cite

show abstract

“…Increased numbers of CD56+ uNK cells have been reported in decidua from women with pre-eclampsia compared to age matched controls (Stallmach et al, 1999;Wilczynski et al, 2003;Bachmayer et al, 2006). In contrast, in our laboratory we have demonstrated a reduction in CD56+ uNK cells in pre-eclampsia in placental bed biopsies (Scaife, Innes, Searle, Robson, Bulmer; unpublished data).…”

Section: Uterine Nk Cells In Pathological Pregnancycontrasting

confidence: 39%

Immune cells in the placental bed

Bulmer¹,

Williams²,

Lash³

2010

Int. J. Dev. Biol.

319

280

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“…Most of these (IL-1␤, IFN-␥, TNF-␣, IL-12, IL-4, IL-10, and IL-2) have been reported to be expressed by human trophoblasts (23)(24)(25)(26), although in a number of different treatment/culture paradigms as well as cytokine measurement techniques. The diversity among these reports, ours included, likely explains the divergence in cytokine production by trophoblast cells in culture.…”

Section: Resultsmentioning

confidence: 99%

Schistosome Egg Antigens Elicit a Proinflammatory Response by Trophoblast Cells of the Human Placenta

et al. 2013

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f Schistosomiasis affects nearly 40 million women of reproductive age. Many of these women are infected while pregnant and lactating. Several studies have demonstrated transplacental trafficking of schistosome antigens; however, little is known regarding how these antigens affect the developing fetus and placenta. To evaluate the impact of schistosomiasis on trophoblasts of the human placenta, we isolated primary trophoblast cells from healthy placentas delivered at term. These trophoblasts were placed in culture and treated with Schistosoma japonicum soluble egg antigens (SEA) or plasma from S. japonicum-infected pregnant women. Outcomes measured included cytokine production and activation of signal transduction pathways. Treatment of primary human trophoblast cells with SEA resulted in upregulation of the proinflammatory cytokines interleukin 6 (IL-6) and IL-8 and the chemokine macrophage inflammatory protein 1␣ (MIP-1␣). Cytokine production in response to SEA was dose dependent and reminiscent of production in response to other proinflammatory stimuli, such as Toll-like receptor 2 (TLR2) and TLR4 agonists. In addition, the signaling pathways extracellular signal-regulated kinase 1/2 (ERK1/2), Jun N-terminal protein kinase (JNK), p38, and NF-B were all activated by SEA in primary trophoblasts. These effects appeared to be mediated through both carbohydrate and protein epitopes of SEA. Finally, primary trophoblasts cocultured with plasma from S. japonicum-infected pregnant women produced increased levels of IL-8 compared to trophoblasts cocultured with plasma from uninfected pregnant women. We report here a direct impact of SEA on primary human trophoblast cells, which are critical for many aspects of a healthy pregnancy. Our data indicate that schistosome antigens can activate proinflammatory responses in trophoblasts, which might compromise maternal-fetal health in pregnancies complicated by schistosomiasis.

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Aberrant Uterine Natural Killer (NK)‐Cell Expression and Altered Placental and Serum Levels of the NK‐Cell Promoting Cytokine Interleukin‐12 in Pre‐Eclampsia

Cited by 99 publications

References 35 publications

Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy

Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy

Immune cells in the placental bed

Schistosome Egg Antigens Elicit a Proinflammatory Response by Trophoblast Cells of the Human Placenta

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