Aberrant vegetative and reproductive development by overexpression and lethality by silencing of OsHAP3E in rice

Ito, Yukihiro; Thirumurugan, T.; Serizawa, Akiko; Hiratsu, Keiichiro; Ohme‐Takagi, Masaru; Kurata, Nori

doi:10.1016/j.plantsci.2011.04.009

Cited by 42 publications

(29 citation statements)

References 23 publications

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“…Finally, in rice, Os-NF-YB7/L1L has roles during both vegetative and floral meristem development. Os-NF-YB7/L1L1-overexpressing plants have a dwarf phenotype and erected leaves, as well as a dense panicle, abnormal rachis, and double flowers (Ito et al, 2011).…”

Section: Nf-y Transcription Factors In Plantsmentioning

confidence: 99%

The Promiscuous Life of Plant NUCLEAR FACTOR Y Transcription Factors

et al. 2012

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The CCAAT box is one of the most common cis-elements present in eukaryotic promoters and is bound by the transcription factor NUCLEAR FACTOR Y (NF-Y). NF-Y is composed of three subunits, NF-YA, NF-YB, and NF-YC. Unlike animals and fungi, plants have significantly expanded the number of genes encoding NF-Y subunits. We provide a comprehensive classification of NF-Y genes, with a separation of closely related, but distinct, histone fold domain proteins. We additionally review recent experiments that have placed NF-Y at the center of many developmental stress-responsive processes in the plant lineage.

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Section: Nf-y Transcription Factors In Plantsmentioning

confidence: 99%

The Promiscuous Life of Plant NUCLEAR FACTOR Y Transcription Factors

et al. 2012

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“…The LEC1 homologues have similar roles in carrot [21], [22] and Theobroma cacao [23]. Genetic analysis of AtNF-YA5 mutants indicate that it is involved in both ABA and blue light responses, together with LEC1 [24], and in drought resistance [25], similarly to AtNF-YB1 and YB2 and maize ZmNF-YB2 [26], [27]. AtNF-YB2 and AtNF-YB3 are important for flowering [28]–[31], and MtHAP2-1 regulates symbiotic nodules in Medicago truncatula [32].…”

Section: Introductionmentioning

confidence: 98%

Interactions and CCAAT-Binding of Arabidopsis thaliana NF-Y Subunits

et al. 2012

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BackgroundNF-Y is a transcription factor that recognizes with high specificity and affinity the widespread CCAAT box promoter element. It is formed by three subunits: NF-YA and the NF-YB/NF-YC- heterodimer containing histone fold domains (HFDs). We previously identified a large NF-Y gene family in Arabidopsis thaliana, composed of 29 members, and characterized their expression patterns in various plant tissues.MethodsWe used yeast Two-hybrids assays (Y2H), pull-down and Electrophoretic Mobility Shift Assay (EMSA) in vitro experiments with recombinant proteins to dissect AtNF-YB/AtNF-YC interactions and DNA-binding with different AtNF-YAs.ResultsConsistent with robust conservation within HFDs, we show that heterodimerization is possible among all histone-like subunits, including the divergent and related LEC1/AtNF-YB9 and L1L/AtNF-YB6 required for embryo development. DNA-binding to a consensus CCAAT box was investigated with specific AtNF-YB/AtNF-YC combinations and observed with some, but not all AtNF-YA subunits.ConclusionsOur results highlight (i) the conserved heterodimerization capacity of AtNF-Y histone-like subunits, and (ii) the different affinities of AtNF-YAs for the CCAAT sequence. Because of the general expansion of NF-Y genes in plants, these results most likely apply to other species.

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“…Overexpression of Arabidopsis NF-YA4 causes growth reduction [19]; while mimicry lines, with a reduced level of miR169 and increased NF-YA transcript levels, display reduced growth [20]. Other NF-Y subunits are also involved in growth control, since Arabidopsis NF-YB1 overexpression arrests growth of the shoot apical meristem [21], and rice OsHAP3E (a NF-YB9 orthologue) overexpression produces a dwarf phenotype [22]. However, the mechanism by which NF-Y modulates plant growth remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Functional and Transcriptome Analysis Reveals an Acclimatization Strategy for Abiotic Stress Tolerance Mediated by Arabidopsis NF-YA Family Members

Ibarra-Laclette²,

et al. 2012

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Nuclear Factor Y (NF-Y) is a heterotrimeric complex formed by NF-YA/NF-YB/NF-YC subunits that binds to the CCAAT-box in eukaryotic promoters. In contrast to other organisms, in which a single gene encodes each subunit, in plants gene families of over 10 members encode each of the subunits. Here we report that five members of the Arabidopsis thaliana NF-YA family are strongly induced by several stress conditions via transcriptional and miR169-related post-transcriptional mechanisms. Overexpression of NF-YA2, 7 and 10 resulted in dwarf late-senescent plants with enhanced tolerance to several types of abiotic stress. These phenotypes are related to alterations in sucrose/starch balance and cell elongation observed in NF-YA overexpressing plants. The use of transcriptomic analysis of transgenic plants that express miR169-resistant versions of NF-YA2, 3, 7, and 10 under an estradiol inducible system, as well as a dominant-repressor version of NF-YA2 revealed a set of genes, whose promoters are enriched in NF-Y binding sites (CCAAT-box) and that may be directly regulated by the NF-Y complex. This analysis also suggests that NF-YAs could participate in modulating gene regulation through positive and negative mechanisms. We propose a model in which the increase in NF-YA transcript levels in response to abiotic stress is part of an adaptive response to adverse environmental conditions in which a reduction in plant growth rate plays a key role.

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Aberrant vegetative and reproductive development by overexpression and lethality by silencing of OsHAP3E in rice

Cited by 42 publications

References 23 publications

The Promiscuous Life of Plant NUCLEAR FACTOR Y Transcription Factors

The Promiscuous Life of Plant NUCLEAR FACTOR Y Transcription Factors

Interactions and CCAAT-Binding of Arabidopsis thaliana NF-Y Subunits

Functional and Transcriptome Analysis Reveals an Acclimatization Strategy for Abiotic Stress Tolerance Mediated by Arabidopsis NF-YA Family Members

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