Aberrant wound healing in the horse: Naturally occurring conditions reminiscent of those observed in man

Theoret, Christine L.; Wilmink, Jacintha M.

doi:10.1111/wrr.12018

Cited by 72 publications

(89 citation statements)

References 38 publications

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“…Apesar da importância do TFG-β1 em diferentes fases do processo de cicatrização da pele, esse fator de crescimento também está associado com o desenvolvimento de tecido de granulação exuberante devido a sua função pró--fibrótica (Theoret et al 2001, van den Boom et al 2002, Theoret & Wilmink 2013, portanto a sua permanência elevada no final do processo de cicatrização cutânea pode não ser benéfica. Entretanto, ao que parece essa redução na pele é lenta, já que no presente estudo os valores do TGF-β1 permaneceram significativamente altos em relação ao tempo zero (0,94±0,69pg/g), tanto no grupo tratado (24,29±12,67pg/g) como controle (36,31±16,59pg/g), porém não houve a formação de tecido de granulação exuberante.…”

Section: Discussionunclassified

“…Entretanto, ao que parece essa redução na pele é lenta, já que no presente estudo os valores do TGF-β1 permaneceram significativamente altos em relação ao tempo zero (0,94±0,69pg/g), tanto no grupo tratado (24,29±12,67pg/g) como controle (36,31±16,59pg/g), porém não houve a formação de tecido de granulação exuberante. Essa formação de tecido está intimamente relacionada com a inflamação crônica da pele, o que perpetua a liberação de mediadores como o TGF-β, que por sua vez estimula de forma excessiva a fibroplasia, ocasionando a formação do tecido (Theoret & Wilmink 2013), o que contraditoriamente inibe a contração (Wilmink & van Weeren 2005). Na realidade, estudos in vitro demonstraram que baseado no modelo de expressão dos genes do TGF-β, o efeito desse fator de crescimento no processo de cicatrização cutânea é paradoxo.…”

Section: Discussionunclassified

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Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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ABSTRACT.-Souza M.V., Pinto J.O., Costa M.M., Santos E.C., Garcia S.L.R. & Oliveira L.L. 2014.[Quantification of growth factors in horse skin treated with platelet-rich plasma.] Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas. Pesquisa Veterinária Brasileira 34(6):599-612. Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário s/n, Viçosa, MG 36570-000, Brazil. E-mail: msouza@ufv.br Platelet-rich plasma (PRP) is a product derived from total blood centrifugation, rich in bioactive factors, such as growth factors. Despite largely used in healing processes, there is a controversy whether the therapy is effective in promoting skin healing. The objective of this study was to quantify and compare the concentrations of the factors TGF-β1 and PDGF-BB in PRP, blood plasma and skin, at different phases of the healing process of skin treated or not with PRP. Seven healthy crossbred 16 to 17-year-old geldings (16.14±0.63) were used. Three quadrangular-shaped lesions (6.25cm 2 ) were surgically induced into the right and left gluteal regions of all animals. Twelve hours after induction of the wounds, 0.5mL of the PRP was administered in each of the four edges of the wound in one of the gluteal regions (Treated group = TG) randomly chosen. The contralateral region was used as control (CG). The wounds were submitted to daily cleaning with Milli-Q water, and samples were obtained with a 6mm diameter biopsy Punch. Six skin biopsies were obtained, the first carried out immediately after the production of the wound (T0), and the others 1 (T1), 2 (T2), 7 (T3), and 14 (T4) days after the lesion was induced. The sixth biopsy (T5) was obtained after complete healing of the skin, which occurred at about day 37 (36.85±7.45, CG; 38.85±6.46, TG). EDTA blood samples were also obtained, at all the times mentioned. Quantification of TGF-β1 and PDGF-BB growth factors on the skin, PRP, and blood plasma was carried out by the ELISA technique. Data were statistically analyzed by the t test, Pearson correlation and regression, at a significance level of 5%. No difference was found between the groups in the values of the two growth factors measured on the skin, at the different times. Also, no correlation was found between the amount of growth factors present in the skin and plasma. On the other hand, a positive correlation was observed between PRP and skin in the treated group, for the growth factors TGF-β1 (r=0.31) and PDGF-BB (r=0.38), as well as between both growth factors present in PRP (r=0.81). Considering the growth factor concentrations at T0, the highest skin values (p<0.05) of TGF-β1, in both groups, occurred at T3 and T5. Higher values (p<0.05) of PDGF-BB occurred at T4 (TG) and T5 (CG). No plasma changes occurred at the concentration of these factors in relation to T0, suggest-1 Recebido em 5 de outubro de 2013.Aceito para publicação em 5 de junho de 2014.

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Section: Discussionunclassified

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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“…For instance, in animals with a more “loose” skin such as rats and mice, healing occurs very rapidly as a result of wound contraction initiated by the musculus panniculus carnosus in their subcutaneous tissues. On the other hand, in “tight-skinned” species such as human and porcine, which lack this muscle, wound contraction is not rapid thus skin heals mainly through re-epithelialization [13,14]. Striking differences in wound healing can even be exhibited within the same species, and have been observed at gross, cellular and molecular level.…”

Section: Mammalian Skin and Wound Healingmentioning

confidence: 99%

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Borena

Martens

Broeckx

et al. 2015

Cell Physiol Biochem

172

112

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Mammal skin has a crucial function in several life-preserving processes such as hydration, protection against chemicals and pathogens, initialization of vitamin D synthesis, excretion and heat regulation. Severe damage of the skin may therefore be life-threatening. Skin wound repair is a multiphased, yet well-orchestrated process including the interaction of various cell types, growth factors and cytokines aiming at closure of the skin and preferably resulting in tissue repair. Regardless various therapeutic modalities targeting at enhancing wound healing, the development of novel approaches for this pathology remains a clinical challenge. The time-consuming conservative wound management is mainly restricted to wound repair rather than restitution of the tissue integrity (the so-called “restitutio ad integrum”). Therefore, there is a continued search towards more efficacious wound therapies to reduce health care burden, provide patients with long-term relief and ultimately scarless wound healing. Recent in vivo and in vitro studies on the use of skin wound regenerative therapies provide encouraging results, but more protracted studies will have to determine whether the effect of observed effects are clinically significant and whether regeneration rather than repair can be achieved. For all the aforementioned reasons, this article reviews the emerging field of regenerative skin wound healing in mammals with particular emphasis on growth factor- and stem cell-based therapies.

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“…The most commonly affected areas are the distal regions of the members, including the carpus and tarsus and pectoral regions (WILMINK & VAN WEEREN, 2004), which suffer higher contamination and mobility as well as low shrinkage rates and blood supply related to the absence of a fleshy panicle, predisposing the formation of exuberant granulation tissue (TGE) (HENDRICKSON & VIRGIN, 2005;THEORET & WILMINK, 2013).…”

Section: Introductionmentioning

confidence: 99%

Copaiba oil in experimental wound healing in horses

et al. 2017

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The aim of this study was to evaluate the effects of 10% copaiba oil in experimentally induced wounds in horses. Four wounds were made in the lumbar and metacarpal regions of eight adult horses. In the treatment group, the wounds received 10% copaiba oil and in the control group 0.9% sodium chloride, in the daily dressing for 21 days. The wounds were evaluated three, 7, 14, and 21 days postoperatively. No significant differences were observed between the groups. The mean lumbar wound contraction rates were 80.54% and 69.64%, for the control and treated groups, respectively. For the wounds in the metacarpal region, these averages were 44.15% and 52.48%, respectively. Under the experimental conditions of the present study, it is concluded that 10% copaiba oil has beneficial in wound healing in the equine species and suggest that copaiba oil can be used as a therapeutic possibility in equine wound therapy.

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Aberrant wound healing in the horse: Naturally occurring conditions reminiscent of those observed in man

Cited by 72 publications

References 38 publications

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Copaiba oil in experimental wound healing in horses

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