ABI4 Activates <i>DGAT1</i> Expression in Arabidopsis Seedlings during Nitrogen Deficiency

Yang, Yang; Yu, Xiangchun; Song, Lian-Fen; Chen, An

doi:10.1104/pp.111.175950

Cited by 110 publications

(104 citation statements)

References 59 publications

(60 reference statements)

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“…Consistent with this, lateral root formation in abi4 is no longer affected by exogenous cytokinin. ABI4 appears to be also required for lipid biosynthesis in seedlings during nitrogen deficiency (Yang et al, 2011). In our study here, we showed that ABI4 plays an important role in regulating root growth.…”

Section: Abi4 Regulates Root Growth In a Dosage-dependent Mannersupporting

confidence: 64%

SCARECROW Has a SHORT-ROOT-Independent Role in Modulating the Sugar Response

2012

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Sugar is essential for all cellular activities, but at high levels it inhibits growth and development. How plants balance the tradeoffs between the need for sugars and their growth inhibitory effects is poorly understood. SHORT-ROOT (SHR) and SCARECROW (SCR) are key regulators of stem cell renewal and radial patterning in the root of Arabidopsis (Arabidopsis thaliana). Recently, we identified direct targets of SHR at the genome scale. Intriguingly, among the top-ranked list, we found a number of genes that are involved in stress responses. By chromatin immunoprecipitation-polymerase chain reaction (PCR), we showed that SHR and SCR regulate a similar but not identical set of stress response genes. Consistent with this, scr and shr were found to be hypersensitive to abscisic acid (ABA). We further showed that both mutants were hypersensitive to high levels of glucose (Glc) but responded normally to high salinity and osmoticum. The endogenous levels of sucrose, Glc, and fructose were also elevated in shr and scr. Intriguingly, although shr had sugar content and developmental defects similar to those of scr, it was much less sensitive to Glc. Chromatin immunoprecipitation-PCR and reverse transcription-PCR assays as well as transgenic studies with an ABA-INSENSITIVE2 (ABI4)-b-glucuronidase reporter construct revealed that in root, SCR, but not SHR, repressed ABI4 and ABI5 directly and specifically in the apical meristem. When combined with abi4, scr became much more tolerant of high Glc. Finally, transgenic plants expressing ABI4 under the control of the SCR promoter manifested a shortroot phenotype. These results together suggest that SCR has a SHR-independent role in mitigating the sugar response and that this role of SCR is important for root growth.

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Section: Abi4 Regulates Root Growth In a Dosage-dependent Mannersupporting

confidence: 64%

SCARECROW Has a SHORT-ROOT-Independent Role in Modulating the Sugar Response

2012

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“…Among 113 potential triacylglycerol biosynthesis genes, 10 had putative loss-of-function frameshifts due to large-effect SNP and/or indels, including the DGAT homolog (Glyma16g21960, GmDGAT2B) and a homolog of Abscisic Acid Insensitive 4 (ABI4) (Glyma14g06290), a transcription factor that regulates triacylglycerol synthesis in Arabidopsis by activating DGAT1 expression during nitrogen deficiency (Supplementary Fig. 21) 39 . A frame-shift mutation in GmDGAT2B was previously found to be associated with reduced seed oil concentration 40 .…”

Section: Npgmentioning

confidence: 99%

De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits

et al. 2014

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Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the establishment and analysis of a pan-genome of Glycine soja, the wild relative of cultivated soybean Glycine max, by sequencing and de novo assembly of seven phylogenetically and geographically representative accessions. Intergenomic comparisons identified lineage-specific genes and genes with copy number variation or large-effect mutations, some of which show evidence of positive selection and may contribute to variation of agronomic traits such as biotic resistance, seed composition, flowering and maturity time, organ size and final biomass. Approximately 80% of the pan-genome was present in all seven accessions (core), whereas the rest was dispensable and exhibited greater variation than the core genome, perhaps reflecting a role in adaptation to diverse environments. This work will facilitate the harnessing of untapped genetic diversity from wild soybean for enhancement of elite cultivars.

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“…During nitrogen deficiency, ABI4 (abscisic acid insensitive4), an AP2 (activator protein-2) type transcription factor, activated the expression of the DGAT1 (acyl-coenzyme A:diacyglycerol acytransferase 1) gene, which is the rate-limiting enzyme in triacylglycerol biosynthesis. ABI4 binds the CE1-like cis element (CACCG) on the DGAT1 promoter that leads to an acceleration of triacylglycerol accumulation in nitrogen limited plants (Yang et al 2011).…”

Section: Nitrogenmentioning

confidence: 99%

Transcriptional Regulatory Components Responding to Macronutrient Limitation

Shin

2011

J. Plant Biol.

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The mechanisms of how plants sense nutrient availability in soils and then send the signals through downstream signal cascades are essential processes for plant survival. Although understanding the regulatory mechanisms of macronutrient sensing and signaling are very important, many components still remain unknown. This review describes the recent progress in identifying the transcriptional regulatory components that respond to phosphorus, nitrogen, potassium, and sulfur limitation. Under phosphorus limited conditions, a MYB transcription factor, PHR1, functions as a key transcriptional regulator of the phosphorus deficient responsive genes via binding to the P1BS cis element. Similarly, SLIM1 plays crucial roles in the expression of the sulfur starved responsive genes through binding to the sulfur responsive cis element. The nitrogen deficient responsive MYB transcription factors control nitrogen metabolism. Cytokinins and microRNAs (miRNAs) also mediate macronutrient metabolic processes. Much less is known about the potassium deficient signaling components, as compared to the other nutrient signaling pathways. Multiple recent studies have suggested that phytohormones, such as ethylene, auxin, jasmonic acid, and abscisic acid (ABA), and reactive oxygen species (ROS) play roles as transcriptional regulatory components in potassium deficient signaling.

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ABI4 Activates DGAT1 Expression in Arabidopsis Seedlings during Nitrogen Deficiency

Cited by 110 publications

References 59 publications

SCARECROW Has a SHORT-ROOT-Independent Role in Modulating the Sugar Response

SCARECROW Has a SHORT-ROOT-Independent Role in Modulating the Sugar Response

De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits

Transcriptional Regulatory Components Responding to Macronutrient Limitation

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