ABI5 regulates ABA-induced anthocyanin biosynthesis by modulating the MYB1-bHLH3 complex in apple

An, Jianping; Zhang, Xiaowei; Liu, Ya‐Jing; Wang, Xiaofei; You, Chun‐Xiang; Hao, Yu‐Jin

doi:10.1093/jxb/eraa525

Cited by 102 publications

(53 citation statements)

References 64 publications

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“…Arakawa et al [26] reported that a rapid increase in anthocyanin synthesis occurred in detached apples after 24 h of light exposure. Further, higher light intensity was reported to induce higher anthocyanin biosynthesis in apples [28]. Although the results of postharvest qualities revealed no significant damages (Table 1) following treatment with a UV-B intensity of 2.1 W•m −2 •S −1 , determining an appropriate light intensity and the duration of irradiation to achieve certain levels of red pigmentation is still required.…”

Section: Accumulation Of Idaein In Two Apple Maturity Stages Owing To...mentioning

confidence: 98%

Pigmentation and Flavonoid Metabolite Diversity in Immature ‘Fuji’ Apple Fruits in Response to Lights and Methyl Jasmonate

Ryu

Duan

Jeong

et al. 2022

IJMS

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Artificial pigmentation of apple fruits has been intensely evaluated to generate less pigmented red apples, which are profitable because of the changes in fruit quality. In this study, we analyzed the diversity of flavonoids and the patterns of flavonoid metabolic gene expression under light irradiation with or without methyl jasmonate (MeJA) treatment in immature (S1) and color-turning (S2) staged ‘Fuji’ apples. Further, we assessed the metabolic regulation at the gene level between anthocyanin and flavonol in light-responsive apple skins. UV-B exposure within 3 days was found to significantly stimulate anthocyanin accumulation in apple skin compared to other light exposure. S1 skin was more sensitive to UV-B and MeJA treatment, in the aspect of indaein accumulation. The enhancement of apple pigmentation following treatment with adequate levels of UV-B and MeJA was maximized at approximately 72 h. Red (range from 4.25 to 17.96 µg·g−1 DW), blue (range from 4.59 to 9.17 µg·g−1 DW) and UV-A (range from 3.98 to 19.12 µg·g−1 DW) lights contributed to the induction of idaein content. Most genes related to the flavonoid pathways increased their expression under UV-B exposure, including the gene expression of the transcription factor, MdMYB10, a well-known upstream factor of flavonoid biosynthesis in apples. The boosted upregulation of MdMYB10, MdCHS, MdF3H MdLDOX, and MdUFGT genes due to MeJA in UV-B was found and may contribute the increase of idaein. UV-A and UV-B caused higher quercetin glycoside content in both S1 and S2 apple skins than longer wavelengths, resulting in significant increases in quercetin-3-O-galactoside and quercetin-3-O-glucoside. These results suggest that the application of adequate UV-B with MeJA in less-pigmented postharvest apples will improve apple color quality within a short period.

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Section: Accumulation Of Idaein In Two Apple Maturity Stages Owing To...mentioning

confidence: 98%

Pigmentation and Flavonoid Metabolite Diversity in Immature ‘Fuji’ Apple Fruits in Response to Lights and Methyl Jasmonate

Ryu

Duan

Jeong

et al. 2022

IJMS

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“…Abscisic acid (ABA), which is synthesized by the key cleavage enzyme 9‐ cis ‐epoxycarotenoid dioxygenase (NCED) in apocarotenoid pathway, has been shown to be a major regulator of ripening in non‐climacteric fruits, such as strawberry, grapes and bilberry (Ferrara et al, 2015; Jia et al, 2011; Karppinen, Tegelberg, Häggman, & Jaakola, 2018) and in climacteric fruits, such as apple (An et al, 2021). The ABA signal transduction is known to be mediated by pyrabactin resistance/like (PYR/PYL) receptors and ABA‐responsive element‐binding factors (ABFs) through SQUAMOSA‐MADS box (TDR‐type) TFs leading to regulation of the MBW complex proteins (Chung et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Red and blue light treatments of ripening bilberry fruits reveal differences in signalling through abscisic acid‐regulated anthocyanin biosynthesis

Samkumar

Jones

Karppinen

et al. 2021

Plant Cell & Environment

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The biosynthesis of anthocyanins has been shown to be influenced by light quality. However, the molecular mechanisms underlying the light‐mediated regulation of fruit anthocyanin biosynthesis are not well understood. In this study, we analysed the effects of supplemental red and blue light on the anthocyanin biosynthesis in non‐climacteric bilberry (Vaccinium myrtillus L.). After 6 days of continuous irradiation during ripening, both red and blue light elevated concentration of anthocyanins, up to 12‐ and 4‐folds, respectively, compared to the control. Transcriptomic analysis of ripening berries showed that both light treatments up‐regulated all the major anthocyanin structural genes, the key regulatory MYB transcription factors and abscisic acid (ABA) biosynthetic genes. However, higher induction of specific genes of anthocyanin and delphinidin biosynthesis alongside ABA signal perception and metabolism were found in red light. The difference in red and blue light signalling was found in 9‐cis‐epoxycarotenoid dioxygenase (NCED), ABA receptor pyrabactin resistance‐like (PYL) and catabolic ABA‐8'hydroxylase gene expression. Red light also up‐regulated expression of soluble N‐ethylmaleimide‐sensitive factor attachment protein receptor (SNARE) domain transporters, which may indicate involvement of these proteins in vesicular trafficking of anthocyanins during fruit ripening. Our results suggest differential signal transduction and transport mechanisms between red and blue light in ABA‐regulated anthocyanin and delphinidin biosynthesis during bilberry fruit ripening.

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“…The bHLH proteins form one of the largest transcription factor families in plants. The anthocyanin biosynthesis-related SGIIIf bHLH genes have been identified in horticultural crops, including those encoding bHLH3 and bHLH64 transcription factors in apple and pear 58 , 59 . The R2R3-MYB transcription factor genes SlAN2-like and BrPAP1a encode regulators of anthocyanin production in tomato and turnip 60 , 61 .…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of the transcriptome, sRNAome, and degradome reveals the network regulating fruit skin coloration in sponge gourd (Luffa cylindrica)

Sun

Zhang

Wang

et al. 2022

Sci Rep

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Sponge gourd fruit skin color is an important quality-related trait because it substantially influences consumer preferences. However, little is known about the miRNAs and genes regulating sponge gourd fruit skin coloration. This study involved an integrated analysis of the transcriptome, sRNAome, and degradome of sponge gourd fruit skins with green skin (GS) and white skin (WS). A total of 4,331 genes were differentially expressed between the GS and WS, with 2,442 down-regulated and 1,889 up-regulated genes in WS. The crucial genes involved in chlorophyll metabolism, chloroplast development, and chloroplast protection were identified (e.g., HEMA, CHLM, CRD1, POR, CAO, CLH, SGR, CAB, BEL1-like, KNAT, ARF, and peroxidase genes). Additionally, 167 differentially expressed miRNAs were identified, with 70 up-regulated and 97 down-regulated miRNAs in WS. Degradome sequencing identified 125 differentially expressed miRNAs and their 521 differentially expressed target genes. The miR156, miR159, miR166, miR167, miR172, and miR393 targeted the genes involved in chlorophyll metabolism, chloroplast development, and chloroplast protection. Moreover, a flavonoid biosynthesis regulatory network was established involving miR159, miR166, miR169, miR319, miR390, miR396, and their targets CHS, 4CL, bHLH, and MYB. The qRT-PCR data for the differentially expressed genes were generally consistent with the transcriptome results. Subcellular localization analysis of selected proteins revealed their locations in different cellular compartments, including nucleus, cytoplasm and endoplasmic reticulum. The study findings revealed the important miRNAs, their target genes, and the regulatory network controlling fruit skin coloration in sponge gourd.

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ABI5 regulates ABA-induced anthocyanin biosynthesis by modulating the MYB1-bHLH3 complex in apple

Cited by 102 publications

References 64 publications

Pigmentation and Flavonoid Metabolite Diversity in Immature ‘Fuji’ Apple Fruits in Response to Lights and Methyl Jasmonate

Pigmentation and Flavonoid Metabolite Diversity in Immature ‘Fuji’ Apple Fruits in Response to Lights and Methyl Jasmonate

Red and blue light treatments of ripening bilberry fruits reveal differences in signalling through abscisic acid‐regulated anthocyanin biosynthesis

Integrated analysis of the transcriptome, sRNAome, and degradome reveals the network regulating fruit skin coloration in sponge gourd (Luffa cylindrica)

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