Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation

Rainho, Claudia Ramos de; Kaezer, Andréa; Aiub, Cláudia Alessandra Fortes; Felzenszwalb, Israel

doi:10.1590/s0001-37652010000400015

Cited by 16 publications

(13 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another plant used in the present study was T. pallida. However, few studies have used this species to evaluate the effects of pesticides (Raino et al, 2010) because it is most commonly used to evaluate environmental samples (Savóia et al, 2009;Crispim et al, 2012;Pereira et al, 2013). The results involving T. pallida indicate significant dose-dependent increases in the frequencies of MN in the groups exposed to the three IMI concentrations.…”

Section: Discussionmentioning

confidence: 99%

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

et al. 2015

View full text Add to dashboard Cite

The indiscriminate use of pesticides has become a serious environmental concern. Of them, imidacloprid (IMI) is one of the most widely used worldwide. In 2010 in Brazil, 1.934 tonnes of IMI were sold and mainly used for sugarcane crops. Several studies have examined the toxicity of IMI as well as its possible ecological effects. However, few studies have examined its toxicity at the genetic level. This is one of the biggest challenges for the scientific community, which is concerned about the impacts of these contaminants on the environment and human health. In this study, we evaluated the effects of IMI above the genetic material in Allium cepa and Tradescantia pallida following exposure to different concentrations of this insecticide. The results demonstrated that the concentrations tested induced chromosomal alterations and increased the frequency of micronuclei. Therefore, IMI in these concentrations was genotoxic to the tested organisms. These factors should be taken into account when applying this pesticide.

show abstract

Section: Discussionmentioning

confidence: 99%

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The roots were exposed to treatment with organic extract and the controls for 24 h. Afterwards, the roots were removed from the bulbs and hydrolyzed with HCl 1N : acetic acid 45% (1 : 9) at 50°C for 5 min. The hydrolyzed root tips were squashed and stained with 2% aceto-orcein [24, 25]. Cytological abnormalities like micronuclei (MCN), bridges, breaks, and laggards were analyzed.…”

Section: Methodsmentioning

confidence: 99%

Genotoxicity of Polycyclic Aromatic Hydrocarbons and Nitro-Derived in Respirable Airborne Particulate Matter Collected from Urban Areas of Rio de Janeiro (Brazil)

Rainho

Corrêa

Mazzei

et al. 2013

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Air pollution toxic effects are mainly attributed to small inhalable particulates with an aerodynamic diameter of less than 2.5 µm (PM 2.5). Our objective was to investigate mutagenic and clastogenic activity in PM samples collected in Rio de Janeiro. Samples were collected using a high-volume sampler at three sites: with low traffic and (2) and (3) with a heavy traffic. Six polycyclic aromatic hydrocarbons (PAHs) were quantified by gas chromatography/mass spectrometry (GC/MS). Salmonella typhimurium TA98 and the derivative strains YG1021 and YG1024 were used in mutagenicity assays in the presence of organic extracts (10–50 µg/ plate) with and without exogenous metabolization. Allium cepa test was performed to evaluate possible cytotoxic and clastogenic activities. The highest PM 2.5 µm (132.73 µm/m3) and PAH values (1.22 ng/m3 for benzo(a)pyrene) were detected at site 3. High mutagenic frameshift responses in absence and presence of metabolic activation were detected at site 3. The participation of nitroarenes and dinitroarenes was detected in the total mutagenicity of the extracts studied. The cytotoxic effect and the abnormalities detected by Allium cepa test can be attributed to the PAH nitroderivatives in the organic extracts. Evaluation of the genotoxicity of urban airborne particulate matter is important as a basis for decision making by regulatory authorities.

show abstract

“…The mutagenicity and genotoxicity evaluation, using more than one test system, is often pointed out [12]. In our study we combined two tests to check for the genotoxicity and mutagenicity of the radiation sterilized disposable burette transfusion apparatus.The tests of gene mutation and chromosome aberration which directly reflect mutagenicity of toxic chemicals are reliable methods to evaluate mutagenicity of toxic chemicals [9,13,14].…”

Section: Discussionmentioning

confidence: 99%

Study on tissue compatibility and genotoxicity of a radiation sterilized disposable burette transfusion apparatus

Chen

Liu

2011

Proceedings 2011 International Conference on Human Health and Biomedical Engineering

View full text Add to dashboard Cite

The aim of the study was to evaluate the effect of radiation sterilization on biocompatibility of a disposable burette transfusion apparatus, in order to assess the safety of radiation sterilized medical devices. The initial bacteria burden of the burette transfusion apparatus was examed according to the ISO11737 standard, we combined cytotoxicity test, sensitization test and dermal irritation test to evaluate the tissue compatibility of the radiation sterilized samples, and the genotoxicity was investigated with chromosomal aberration test and micronucleus test. The study determined the verified sterilizing dosage at the level of 9.4kGy, results show the sample products had no cytotoxicity, no sensitization,no intracutaneous irritation, no genotoxicity in chromosome aberration test, and will not increase the micronucleus rate of PCE (p<0.05). Disposable burette transfusion apparatus showed good tissue compatibility and had no genotoxicity after radiation sterilization. Radiation sterilization is a biologically safe method for sterilization of medical devices.

show abstract

Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation

Cited by 16 publications

References 34 publications

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

Genotoxicity of Polycyclic Aromatic Hydrocarbons and Nitro-Derived in Respirable Airborne Particulate Matter Collected from Urban Areas of Rio de Janeiro (Brazil)

Study on tissue compatibility and genotoxicity of a radiation sterilized disposable burette transfusion apparatus

Contact Info

Product

Resources

About