Ability of Polymorphonuclear Leukocytes to Generate Active Oxygen Species in Children with Bronchial Asthma

Katô, Masahiko; Nakano, Minoru; Morikawa, Akihiro; Kimura, Hirokazu; Shigeta, Makoto; Kuroume, Takayoshi

doi:10.1159/000235448

Cited by 29 publications

(15 citation statements)

References 15 publications

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“…Our results obtained for PMNs in asthma are in agreement with those obtained by KATO et al [22], and NEIJFENS et al [5]. The results of KATO et al [22] suggested that PMNs in asthmatic children, especially those with attacks, generate more ROS than those of healthy subjects, as assessed by a cypridina luciferin analogue enhanced CL.…”

Section: Discussionsupporting

confidence: 92%

“…The results of KATO et al [22] suggested that PMNs in asthmatic children, especially those with attacks, generate more ROS than those of healthy subjects, as assessed by a cypridina luciferin analogue enhanced CL. NEIJFENS et al [5] suggested that the increased generation of ROS in children with asthma might also contribute to causing bronchial hyperresponsiveness (BHR).…”

Section: Discussionmentioning

confidence: 99%

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Enhancement of reactive oxygen species formation in stable and unstable asthmatic patients

Vachier

Chanez²,

Doucen³

et al. 1994

Eur Respir J

107

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E En nh ha an nc ce em me en nt t o of f r re ea ac ct ti iv ve e o ox xy yg ge en n s sp pe ec ci ie es s f fo or rm ma at ti io on n i in n s st ta ab bl le e a an nd d u un ns st ta ab bl le e a as st th hm ma at ti ic c p pa at ti ie en nt ts s ABSTRACT: There is increasing evidence to suggest that human blood polymorphonuclear neutrophils (PMNs) and monocytes play an important role in the inflammatory processes of asthma. In asthmatic patients, PMNs and monocytes were shown to be activated more than in healthy subjects. We investigated the capacity of these two cell populations to generate reactive oxygen species (ROS) in stable and unstable asthmatic patients. The two populations of asthmatic patients were identified by asthma activity, as expressed by clinical events occurring within 2 weeks prior to the study. Oxygen species formation was analysed for isolated purified PMNs and monocytes (Mos) by chemiluminescence (CL) using lucigenin and luminol as luminescent probes. CL was determined on nonstimulated and on phorbol myristate acetate (PMA)-stimulated cells. The stimulatability coefficient (PMA-stimulated/nonstimulated cell ratio) of each cell population was then calculated.Resting PMNs and Mos generated significantly greater amounts of ROS in stable asthmatic patients, and much more in unstable asthmatic patients, as compared to healthy subjects, both in lucigenin and luminol enhanced CL. Non O 2 · -ROS production from PMA-stimulated PMNs and Mos was identical in unstable asthmatic patients and in healthy subjects, whereas a significant decrease was observed in stable asthmatic patients, as assessed by luminol enhanced CL. PMA-stimulated cells showed no difference in O 2 · -generation, as assessed by lucigenin enhanced CL. However, the stimulatability coefficient of all asthmatic patients was always significantly lower than that of healthy subjects.These results suggest that there are differences in priming and stimulation of Ros production from PMNs and Mos between stable and unstable asthmatic patients. Release of oxygen species from these cells may be implicated in the pathophysiology of unstable asthma.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Enhancement of reactive oxygen species formation in stable and unstable asthmatic patients

Vachier

Chanez²,

Doucen³

et al. 1994

Eur Respir J

107

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show abstract

Section: Discussionmentioning

confidence: 99%

“…A correlation between neutrophil ROM production and bronchial hyperreactivity has been shown both in bronchial asthma [17,19], and in chronic obstructive pulmonary disease [32]. The magnitude of the neutrophil ROM responses has been reported to correlate with the severity of asthma symptoms [19]. The chemiluminescence signal obtained from whole blood is predominantly a function of neutrophil number and function [33], whilst the contribution of monocytes, lymphocytes and thrombocytes has been considered negligible [22,[34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

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Increased PMA-induced chemiluminescence from whole blood of patients with bronchial hyperreactivity

Nordman¹,

Nyberg²,

Linko³

1994

Eur Respir J

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Blood phagocytes from patients with asthma have an increased capacity to produce reactive oxygen metabolites. We studied whether whole blood luminol-dependent chemiluminescence could detect this phenomenon in patients with a normal spirometry but bronchial hyperreactivity as determined with a methacholine bronchial challenge test. Whole blood chemiluminescence, serum eosinophilic cationic protein (ECP), and serum myeloperoxidase (MPO) were determined from 50 patients referred for a methacholine challenge due to prolonged cough and/or dyspnoea. The chemiluminescence results were compared to those from 15 healthy persons. The hyperreactive patients (n = 18) had significantly higher phorbol 12-myristate 13-acetate (PMA)-induced whole blood chemiluminescence values (mean 18.8 mV.min-1; 95% confidence limits (C.L.) 16.3-21.3 mV.min-1) than the normoreactive patients (mean 14.2 mV.min-1; 95% C.L. 13.0-15.5 mV.min-1;) and the healthy controls (mean 12.8 mV.min-1; 95% C.L. 11.7-13.9 mV.min-1). There was no significant difference in PMA-induced chemiluminescence between the normoreactive patients and the controls. The hyperreactive patients had higher serum ECP values than the normoreactive patients, but there was no correlation between whole blood chemiluminescence and serum ECP levels or total eosinophil count. There was no significant difference in monocyte reactive oxygen metabolite production or serum MPO values between the normoreactive and the hyperreactive patients. We suggest that the increased PMA-induced whole blood chemiluminescence in bronchial hyperreactivity is due mainly to an activation of neutrophils, and that the assay might be useful as a systemic inflammatory marker in patients with pulmonary inflammatory processes resulting in bronchial hyperreactivity.

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Effects of CIS-19, a novel PAF receptor antagonist, on PAF-induced eosinophil recruitment and enhancement of superoxide anion generation in guinea-pigs

Teng

Lin

Kuo

et al. 1995

Naunyn-Schmiedeberg's Arch Pharmacol

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We examined the effects of a novel platelet-activating factor (PAF) receptor antagonist, CIS-19 [cis-2-(3,4-dimethoxyphenyl)-6-isopropoxy-7-methoxyl-1-(N-methylforma mido)- 1,2,3,4-tetrahydronaphthalene], on PAF-induced inflammatory cells recruitment into airways and enhancement of superoxide anion (O2-) generation from cells retrieved by bronchoalveolar lavage (BAL) in urethane-anesthetized guinea-pigs. Administration of PAF (30 ng/kg, i.v.) produced a selective increase of eosinophils into airways, but no significant increase of the number of macrophages, neutrophils or lymphocytes. CIS-19 (2.5 and 5 mg/kg, i.v.) significantly inhibited the eosinophil recruitment induced by PAF. In vitro, PAF, phorbol 12-myristate 13-acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) directly stimulated generation of O2- from BAL cells in a concentration-dependent manner. CIS-19 (10(-7)-10(-4) M) inhibited production of O2- induced by PAF (10(-7) M) in a concentration-dependent manner with an EC50 value of 0.84 microM, but not induced by PMA (0.5 microgram/ml) or FMLP (10(-7) M). Administration of PAF (5 ng/kg, i.v.) enhanced markedly PMA (0.5 microgram/ml) and FMLP (10(-7) M)-induced generation of O2- by 80.2% and 51.3%, respectively. The enhancing effect of PAF was maximal in cells harvested 5 min after the addition of PAF and then declined to baseline level at 60 min. These responses were inhibited by administration of CIS-19 (0.5-2.5 mg/kg, i.v.) or BN 52021 (5 mg/kg, i.v.). The results indicate that CIS-19 is potent in inhibition of PAF-induced airway inflammatory response and may have therapeutic potential as an anti-inflammatory drugs.

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Ability of Polymorphonuclear Leukocytes to Generate Active Oxygen Species in Children with Bronchial Asthma

Cited by 29 publications

References 15 publications

Enhancement of reactive oxygen species formation in stable and unstable asthmatic patients

Enhancement of reactive oxygen species formation in stable and unstable asthmatic patients

Increased PMA-induced chemiluminescence from whole blood of patients with bronchial hyperreactivity

Effects of CIS-19, a novel PAF receptor antagonist, on PAF-induced eosinophil recruitment and enhancement of superoxide anion generation in guinea-pigs

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