Abiotic stress-induced oscillations in steady-state transcript levels of Group 3<i>LEA</i>protein genes in the moss,<i>Physcomitrella patens</i>

Shinde, Suhas; Shinde, Rupali; Downey, Frances; Ng, Carl

doi:10.4161/psb.22535

Cited by 8 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gametophores dried gradually, however, did acquire desiccation tolerance and were capable of surviving to −287 MPa. The data do not support the hypothesis that ABA accumulation on its own is sufficient for the acquisition of desiccation tolerance in P. patens , even though exogenous ABA can induce desiccation tolerance when applied to the medium (Koster et al, ; Oldenhof et al, ; Pressel & Duckett, ; Shinde et al, ; Shinde et al, ). Surprisingly, gametophores of the P. patens transgenic line Ppabi3a/b/c where the production of the ABI3 transcription factor is negated were able to acquire some desiccation tolerance when dried gradually (Figure ).…”

Section: Discussionmentioning

confidence: 80%

“…Application of exogenous ABA induces tolerance of rapid desiccation in both protonemata and gametophores of P. patens (Koster et al, ; Oldenhof, Wolkers, Bowman, Tablin, & Crowe, ; Pressel & Duckett, ; Shinde, Nurul, & Ng, ; Shinde, Shinde, Downey, & Ng, ). The importance of ABA signaling pathways in the acquisition of desiccation tolerance has been demonstrated by the generation of knock out mutants of genes encoding proteins in the signaling cascade.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Desiccation tolerance in Physcomitrella patens: Rate of dehydration and the involvement of endogenous abscisic acid (ABA)

Xiao

Yobi

Koster

et al. 2017

Plant Cell & Environment

View full text Add to dashboard Cite

The moss Physcomitrella patens, a model system for basal land plants, tolerates several abiotic stresses, including dehydration. We previously reported that Physcomitrella patens survives equilibrium dehydration to −13 MPa in a closed system at 91% RH. Tolerance of desiccation to water potentials below −100 MPa was only achieved by pretreatment with exogenous abscisic acid (ABA). We report here that gametophores, but not protonemata, can survive desiccation below −100 MPa after a gradual drying regime in an open system, without exogenous ABA. In contrast, faster equilibrium drying at 90% RH for 3-5 days did not induce desiccation tolerance in either tissue. Endogenous ABA accumulated in protonemata and gametophores under both drying regimes, so did not correlate directly with desiccation tolerance. Gametophores of a Ppabi3a/b/ c triple knock out transgenic line also survived the gradual dehydration regime, despite impaired ABA signaling. Our results suggest that the initial drying rate, and not the amount of endogenous ABA, may be critical in the acquisition of desiccation tolerance. Results from this work will provide insight into ongoing studies to uncover the role of ABA in the dehydration response and the underlying mechanisms of desiccation tolerance in this bryophyte.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Desiccation tolerance in Physcomitrella patens: Rate of dehydration and the involvement of endogenous abscisic acid (ABA)

Xiao

Yobi

Koster

et al. 2017

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Quantitative RT-PCR was used to test whether stress-associated, ABA-inducible marker genes were likewise upregulated in CCaMK-IPD3 gain-of-function lines. We selected two previously described marker genes, LEA3-1 and LEA3-2 , which encode late embryogenesis abundant (LEA) proteins ( Shinde et al., 2012 , 2013 ) and confirmed that transcript levels were elevated in wild-type protonemata treated with exogenously supplied ABA ( Figure 5 B). Initially characterized in seeds, LEA proteins serve as osmoprotective molecules and are thought to confer abiotic stress resistance in brood cells, thereby enhancing their dispersal ability ( Figure 5 C).…”

Section: Resultsmentioning

confidence: 60%

Stress-associated developmental reprogramming in moss protonemata by synthetic activation of the common symbiosis pathway

et al. 2022

View full text Add to dashboard Cite

Summary Symbioses between angiosperms and rhizobia or arbuscular mycorrhizal fungi are controlled through a conserved signaling pathway. Microbe-derived, chitin-based elicitors activate plant cell surface receptors and trigger nuclear calcium oscillations, which are decoded by a calcium/calmodulin-dependent protein kinase (CCaMK) and its target transcription factor interacting protein of DMI3 (IPD3). Genes encoding CCaMK and IPD3 have been lost in multiple non-mycorrhizal plant lineages yet retained among non-mycorrhizal mosses. Here, we demonstrated that the moss Physcomitrium is equipped with a bona fide CCaMK that can functionally complement a Medicago loss-of-function mutant. Conservation of regulatory phosphosites allowed us to generate predicted hyperactive forms of Physcomitrium CCaMK and IPD3. Overexpression of synthetically activated CCaMK or IPD3 in Physcomitrium led to abscisic acid (ABA) accumulation and ectopic development of brood cells, which are asexual propagules that facilitate escape from local abiotic stresses. We therefore propose a functional role for Physcomitrium CCaMK-IPD3 in stress-associated developmental reprogramming

show abstract

“…ABA acts as a signaling molecule to activate stress-responsive genes and protective proteins, including some late embryogenesis abundant (LEA) proteins, in vegetative tissues and seeds of different plant species (Lane 1991, Berjak et al 2007, Shinde et al 2013. ABA biosynthesis and some key signaling core components such as ABI3 and ABI1 are well characterized in P. patens (Marella et al 2006, Komatsu et al 2009, Takezawa et al 2015.…”

Section: Discussionmentioning

confidence: 99%

Acclimation and endogenous abscisic acid in the moss Physcomitrella patens during acquisition of desiccation tolerance

Rathnayake

Nelson

Seeve

et al. 2019

Physiologia Plantarum

View full text Add to dashboard Cite

The moss Physcomitrella patens has been used as a model organism to study the induction of desiccation tolerance (DT), but links between dehydration rate, the accumulation of endogenous abscisic acid (ABA) and DT remain unclear. In this study, we show that prolonged acclimation of P. patens at 89% relative humidity (RH) [−16 MPa] can induce tolerance of desiccation at 33% RH (−153 MPa) in both protonema and gametophore stages. During acclimation, significant endogenous ABA accumulation occurred after 1 day in gametophores and after 2 days in protonemata. Physcomitrella patens expressing the ABA‐inducible EARLY METHIONINE promoter fused to a cyan fluorescent protein (CFP) reporter gene revealed a mostly uniform distribution of the CFP increasing throughout the tissues during acclimation. DT was measured by day 6 of acclimation in gametophores, but not until 9 days of acclimation for protonemata. These results suggest that endogenous ABA accumulating when moss cells experience moderate water loss requires sufficient time to induce the changes that permit cells to survive more severe desiccation. These results provide insight for ongoing studies of how acclimation induces metabolic changes to enable DT in P. patens.

show abstract

Abiotic stress-induced oscillations in steady-state transcript levels of Group 3LEAprotein genes in the moss,Physcomitrella patens

Cited by 8 publications

References 19 publications

Desiccation tolerance in Physcomitrella patens: Rate of dehydration and the involvement of endogenous abscisic acid (ABA)

Desiccation tolerance in Physcomitrella patens: Rate of dehydration and the involvement of endogenous abscisic acid (ABA)

Stress-associated developmental reprogramming in moss protonemata by synthetic activation of the common symbiosis pathway

Acclimation and endogenous abscisic acid in the moss Physcomitrella patens during acquisition of desiccation tolerance

Contact Info

Product

Resources

About