Abiotic stress regulates expression of galactinol synthase genes post-transcriptionally through intron retention in rice

Mukherjee, Sritama; Sengupta, Sonali; Mukherjee, Avinandan; Basak, Papri; Majumder, Arun Lahiri

doi:10.1007/s00425-018-3046-z

Cited by 32 publications

(15 citation statements)

References 77 publications

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“…This co-introgression of PcINO1 and its downstream gene assures that the inositol pool is not depleted under stress in coping up with the increasing demand for pinitol synthesis in the transgenic system. An interesting phenomenon of alternative splicing for intron retention of GolS1 and GolS2 transcripts was noted in stressed rice plants (Mukherjee et al 2019b). Characterization of genes and promoters as well as stress-responsiveness of PLCs and MIOX from rice varieties and local landrace cultivars, help in determining the importance of polymorphism among the members of same species (Datta et al; Adak et al; unpublished, this laboratory).…”

Section: Role Of Myo-inositol and Its Metabolic Derivatives From Higher Plants In Evading Stressmentioning

confidence: 99%

Regulation of stress-induced inositol metabolism in plants: a phylogenetic search for conserved cis elements

Basak

Majumder

2021

J. Plant Biochem. Biotechnol.

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Inositol and its' metabolic derivatives such as galactinol, raffinose, methylated inositols etc. are well known significant osmoprotectants produced in response to various abiotic stresses in plants. The present work focuses on the importance of phylogenetic analysis in search of a presumptive 'master switch' involved in inducing a set of stress-responsive coregulated genes in the pathways involved in inositol synthesis and its further metabolism. It can be reasonably assumed that the entire network of genes induced under a particular stress is governed by a few common stress-responsive transcription factor binding sites or cis elements in their respective promoters. A phylogenetic analysis of such regulatory regions of the coregulated genes participating in entire inositol metabolic pathway throughout evolution, can form a novel approach in recognizing the conserved stress-regulatory signature(s) or 'master switch(es)' regulating this important pathway under the stress impact.

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Section: Role Of Myo-inositol and Its Metabolic Derivatives From Higher Plants In Evading Stressmentioning

confidence: 99%

Regulation of stress-induced inositol metabolism in plants: a phylogenetic search for conserved cis elements

Basak

Majumder

2021

J. Plant Biochem. Biotechnol.

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“…This enzyme catalyzes the synthesis of galactinol from inositol and UDP galactose, which then is the substrate for raffinose biosynthesis. Galactinol synthases have been described previously in response to abiotic stress in rice [ 35 , 36 ] and the overexpression of AtGolS2 in rice contributed to increased drought tolerance and yield [ 37 ]. An impact of HNT on daytime water use was recently suggested during the seedling stage for wheat due to higher transpiration rates during the day [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Differentiation of the High Night Temperature Response in Leaf Segments of Rice Cultivars with Contrasting Tolerance

Schaarschmidt

Glaubitz

Erban

et al. 2021

IJMS

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High night temperatures (HNT) affect rice yield in the field and induce chlorosis symptoms in leaves in controlled chamber experiments. However, little is known about molecular changes in leaf segments under these conditions. Transcript and metabolite profiling were performed for leaf segments of six rice cultivars with different HNT sensitivity. The metabolite profile of the sheath revealed a lower metabolite abundance compared to segments of the leaf blade. Furthermore, pre-adaptation to stress under control conditions was detected in the sheath, whereas this segment was only slightly affected by HNT. No unique significant transcriptomic changes were observed in the leaf base, including the basal growth zone at HNT conditions. Instead, selected metabolites showed correlations with HNT sensitivity in the base. The middle part and the tip were most highly affected by HNT in sensitive cultivars on the transcriptomic level with higher expression of jasmonic acid signaling related genes, genes encoding enzymes involved in flavonoid metabolism and a gene encoding galactinol synthase. In addition, gene expression of expansins known to improve stress tolerance increased in tolerant and sensitive cultivars. The investigation of the different leaf segments indicated highly segment specific responses to HNT. Molecular key players for HNT sensitivity were identified.

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“…In addition, several recent reports highlight the role and regulation of the raffinose family oligosaccharides (RFOs) in mitigating abiotic stresses. The molecular functioning of RFOs and their key regulatory enzymes have been elucidated in diverse crop species, including rice, chickpea, maize, and cucumber, to name a few (Li et al, 2020; Ma et al, 2021; Mukherjee et al, 2019; Salvi et al, 2020). Since RFOs are the alfa‐galactosyl derivatives of sucrose, their cellular pool is largely reliant on the sucrose concentration.…”

Section: Introductionmentioning

confidence: 99%

Sugar transporters and their molecular tradeoffs during abiotic stress responses in plants

Salvi

Agarrwal²,

Kajal

et al. 2022

Physiologia Plantarum

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Sugars as photosynthates are well known as energy providers and as building blocks of various structural components of plant cells, tissues and organs. Additionally, as a part of various sugar signaling pathways, they interact with other cellular machinery and influence many important cellular decisions in plants. Sugar signaling is further reliant on the differential distribution of sugars throughout the plant system. The distribution of sugars from source to sink tissues or within organelles of plant cells is a highly regulated process facilitated by various sugar transporters located in plasma membranes and organelle membranes, respectively. Sugar distribution, as well as signaling, is impacted during unfavorable environments such as extreme temperatures, salt, nutrient scarcity, or drought. Here, we have discussed the mechanism of sugar transport via various types of sugar transporters as well as their differential response during environmental stress exposure. The functional involvement of sugar transporters in plant's abiotic stress tolerance is also discussed. Besides, we have also highlighted the challenges in engineering sugar transporter proteins as well as the undeciphered modules associated with sugar transporters in plants. Thus, this review provides a comprehensive discussion on the role and regulation of sugar transporters during abiotic stresses and enables us to target the candidate sugar transporter(s) for crop improvement to develop climate‐resilient crops.

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Abiotic stress regulates expression of galactinol synthase genes post-transcriptionally through intron retention in rice

Cited by 32 publications

References 77 publications

Regulation of stress-induced inositol metabolism in plants: a phylogenetic search for conserved cis elements

Regulation of stress-induced inositol metabolism in plants: a phylogenetic search for conserved cis elements

Differentiation of the High Night Temperature Response in Leaf Segments of Rice Cultivars with Contrasting Tolerance

Sugar transporters and their molecular tradeoffs during abiotic stress responses in plants

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