Abiotic Stress Responsive miRNA-Target Network and Related Markers (SNP, SSR) in Brassica juncea

Singh, Indra; Smita, Shuchi; Mishra, Dwijesh Chandra; Kumar, Sanjeev; Singh, B. K.; Rai, Anil

doi:10.3389/fpls.2017.01943

Cited by 40 publications

(33 citation statements)

References 89 publications

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“…In tolerant soybean however, miR397a/b is down regulated during water stress conditions [112]. This transcript targets β-fructofuranosidase (FFase) and modulates metabolic activity of starch and sucrose in plants [113]. The fluctuation in the levels of miR397 expression (up or down regulation) regulates photosynthesis and respiration.…”

Section: The Role Of Mirnas On Photosynthesis and Respiration Durimentioning

confidence: 99%

Drought Response in Rice: The miRNA Story

Nadarajah

Kumar

2019

IJMS

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As a semi-aquatic plant, rice requires water for proper growth, development, and orientation of physiological processes. Stress is induced at the cellular and molecular level when rice is exposed to drought or periods of low water availability. Plants have existing defense mechanisms in planta that respond to stress. In this review we examine the role played by miRNAs in the regulation and control of drought stress in rice through a summary of molecular studies conducted on miRNAs with emphasis on their contribution to drought regulatory networks in comparison to other plant systems. The interaction between miRNAs, target genes, transcription factors and their respective roles in drought-induced stresses is elaborated. The cross talk involved in controlling drought stress responses through the up and down regulation of targets encoding regulatory and functional proteins is highlighted. The information contained herein can further be explored to identify targets for crop improvement in the future.

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Section: The Role Of Mirnas On Photosynthesis and Respiration Durimentioning

confidence: 99%

Drought Response in Rice: The miRNA Story

Nadarajah

Kumar

2019

IJMS

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“…The involvement of miR5021 family miRNAs has been shown in diverse biological processes related to growth and development, e.g., stress responses, signal transduction, transport, cell division, trichome development, essential oil, and many other metabolic pathways . The members from miR5658 family have been reported to be involved in regulating starch and sugar metabolism, and stress responses .…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNAs and miRNAs regulating terpene and tartaric acid biosynthesis in rose‐scented geranium

2019

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This study aimed to explore the noncoding RNAs, which have emerged as key regulatory molecules in biological processes, in rose‐scented geranium. We analyzed RNA‐seq data revealing 26 784 long noncoding RNAs (lncRNAs) and 871 miRNAs in rose‐scented geranium. A total of 466 lncRNAs were annotated using different plant lncRNA public databases. Furthermore, 372 lncRNAs and 99 miRNAs were detected that target terpene and tartarate biosynthetic pathways. An interactome, comprising of lncRNAs, miRNAs, and mRNAs, was constructed that represents a noncoding RNA regulatory network of the target mRNAs. Real‐time quantitative PCR expression validation was done for selected lncRNAs involved in the regulation of terpene and tartaric acid pathways. This study provides the first insights into the regulatory functioning of noncoding RNAs in rose‐scented geranium.

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“…SSRs within the miRNA precursors have been identified and used for the diversity analysis [16,45]. Heat stress, highly effecting world wheat crop production is an important abiotic stress.…”

Section: Cluster Analysis Of 37 Wheat Genotypesmentioning

confidence: 99%

Development and Use of miRNA-derived SSR Markers to Study Genetic Diversity, Population Structure and Characterization of Genotypes for Heat Tolerance Breeding in Bread Wheat (Triticum aestivumL.)

Tyagi

Kumar

Gautam³

et al. 2020

Preprint

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Wheat is one of the most important cereal crop in the world. Heat stress is an important abiotic stress limiting wheat production and productivity in the world including south-east Asia. The importance of miRNAs in gene expression under various biotic and abiotic stresses is well documented. Molecular markers, especially SSR markers, plays an important role for the success in molecular plant breeding programs. The discovery of SSRs from non-coding regions has been a challenging task. Therefore, development of novel miRNA-based SSRs from the conserved portions of the genome will prove useful for the study of genetic diversity of heat-responsive miRNA-genes in wheat. In the present study, efforts are made to mine SSR markers from 96 members of heat-responsive miRNA-genes of wheat followed by their validation using 37 contrasting (heat tolerance/susceptible) wheat genotypes. Among a set of 13 miRNA-SSRs used,7SSRs were found polymorphic. Among these polymorphic SSR markers, three found to be very informative SSRs (HT-169j, HT-160a and HT-160b) and could largely discriminate heat tolerant genotypes from the heat susceptible ones. Further analysis based on Polymorphism Information Content (PIC) revealed that miRNA genes were more diverse in susceptible genotypes compared to tolerant genotypes. Ours is the first report that the genic/miRNA markers could be successfully used to study wheat diversity, population structure and characterization of trait specific germplasm. The important and useful miRNA-based SSRs, therefore, would serve as best markers in the marker-assisted breeding programs aimed at enhancing heat tolerance of Indian wheat.

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Abiotic Stress Responsive miRNA-Target Network and Related Markers (SNP, SSR) in Brassica juncea

Cited by 40 publications

References 89 publications

Drought Response in Rice: The miRNA Story

Drought Response in Rice: The miRNA Story

Long noncoding RNAs and miRNAs regulating terpene and tartaric acid biosynthesis in rose‐scented geranium

Development and Use of miRNA-derived SSR Markers to Study Genetic Diversity, Population Structure and Characterization of Genotypes for Heat Tolerance Breeding in Bread Wheat (Triticum aestivumL.)

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