Abiotic stress treatment reveals expansin like A gene OfEXLA1 improving salt and drought tolerance of Osmanthus fragrans by responding to abscisic acid

Dong, Bin; Wang, Qianqian; Zhou, Dan; Wang, Yiguang; Miao, Yunfeng; Zhong, Shiwei; Fang, Qiu; Yang, Liyuan; Xiao, Zhen; Zhao, Hongbo

doi:10.1016/j.hpj.2022.11.007

Cited by 11 publications

(3 citation statements)

References 37 publications

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“…EaEXPA1 overexpression improves drought tolerance in transgenic sugarcane ( Narayan et al., 2021 ). Furthermore, in transgenic Arabidopsis , the Osmanthus fragrans OfEXLA1 can improve salt and drought tolerance, which is regulated by the abscisic acid signaling pathway ( Dong et al., 2023 ). When the wild peanut AdEXLB8 gene is heterogeneously expressed in tobacco, it leads to cell wall reorganization, enhancing both drought tolerance and resistance to Sclerotinia sclerotiorum and Meloidogyne incognita ( Brasileiro et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of the expansin gene family in netted melon and their transcriptional responses to fruit peel cracking

Hu,

Li,

Zhu

et al. 2024

Front. Plant Sci.

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IntroductionFruit cracking not only affects the appearance of netted melons (Cucumis melo L. var. reticulatus Naud.) but also decreases their marketability.MethodsHerein, to comprehensively understand the role of expansin (EXP) proteins in netted melon, bioinformatics methods were employed to discover the EXP gene family in the melon genome and analyze its characteristic features. Furthermore, transcriptomics analysis was performed to determine the expression patterns of melon EXP (CmEXP) genes in crack-tolerant and crack-susceptible netted melon varieties.DiscussionThirty-three CmEXP genes were identified. Chromosomal location analysis revealed that CmEXP gene distribution was uneven on 12 chromosomes. In addition, phylogenetic tree analysis revealed that CmEXP genes could be categorized into four subgroups, among which the EXPA subgroup had the most members. The same subgroup members shared similar protein motifs and gene structures. Thirteen duplicate events were identified in the 33 CmEXP genes. Collinearity analysis revealed that the CmEXP genes had 50, 50, and 44 orthologous genes with EXP genes in cucumber, watermelon, and Arabidopsis, respectively. However, only nine orthologous EXP genes were observed in rice. Promoter cis-acting element analysis demonstrated that numerous cis-acting elements in the upstream promoter region of CmEXP genes participate in plant growth, development, and environmental stress responses. Transcriptomics analysis revealed 14 differentially expressed genes (DEGs) in the non-cracked fruit peels between the crack-tolerant variety ‘Xizhoumi 17’ (N17) and the crack-susceptible variety ‘Xizhoumi 25’ (N25). Among the 14 genes, 11 were upregulated, whereas the remaining three were downregulated in N17. In the non-cracked (N25) and cracked (C25) fruit peels of ‘Xizhoumi 25’, 24 DEGs were identified, and 4 of them were upregulated, whereas the remaining 20 were downregulated in N25. In the two datasets, only CmEXPB1 exhibited consistently upregulated expression, indicating its importance in the fruit peel crack resistance of netted melon. Transcription factor prediction revealed 56 potential transcription factors that regulate CmEXPB1 expression.ResultsOur study findings enrich the understanding of the CmEXP gene family and present candidate genes for the molecular breeding of fruit peel crack resistance of netted melon.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification of the expansin gene family in netted melon and their transcriptional responses to fruit peel cracking

Hu,

Li,

Zhu

et al. 2024

Front. Plant Sci.

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“…The ability of EXPs to regulate cell wall modification and elongation means they have crucial functions in multiple biological processes, including response to biotic and abiotic stress, root and fiber development, root nodule formation, fruit development and ripening, and other developmental processes ( Choi et al., 2006 , 2008 ; Cosgrove, 2015b ; Marowa et al., 2016 ). For instance, overexpression of the Osmanthus fragrans OfEXLA1 gene has been shown to increase resistance to salt and drought stress in Arabidopsis ( Dong et al., 2023 ). Ectopic overexpression of wild Arachis AdEXLB8 in tobacco increased tolerances to biotic ( Meloidogyne incognita and Sclerotinia sclerotiorum ) and abiotic (drought) stresses ( Brasileiro et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome- and genome-wide systematic identification of expansin gene family and their expression in tuberous root development and stress responses in sweetpotato (Ipomoea batatas)

Zhang,

Dong,

Zhu

et al. 2024

Front. Plant Sci.

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IntroductionExpansins (EXPs) are essential components of the plant cell wall that function as relaxation factors to directly promote turgor-driven expansion of the cell wall, thereby controlling plant growth and development and diverse environmental stress responses. EXPs genes have been identified and characterized in numerous plant species, but not in sweetpotato.Results and methodsIn the present study, a total of 59 EXP genes unevenly distributed across 14 of 15 chromosomes were identified in the sweetpotato genome, and segmental and tandem duplications were found to make a dominant contribution to the diversity of functions of the IbEXP family. Phylogenetic analysis showed that IbEXP members could be clustered into four subfamilies based on the EXPs from Arabidopsis and rice, and the regularity of protein motif, domain, and gene structures was consistent with this subfamily classification. Collinearity analysis between IbEXP genes and related homologous sequences in nine plants provided further phylogenetic insights into the EXP gene family. Cis-element analysis further revealed the potential roles of IbEXP genes in sweetpotato development and stress responses. RNA-seq and qRT-PCR analysis of eight selected IbEXPs genes provided evidence of their specificity in different tissues and showed that their transcripts were variously induced or suppressed under different hormone treatments (abscisic acid, salicylic acid, jasmonic acid, and 1-aminocyclopropane-1-carboxylic acid) and abiotic stresses (low and high temperature).DiscussionThese results provide a foundation for further comprehensive investigation of the functions of IbEXP genes and indicate that several members of this family have potential applications as regulators to control plant development and enhance stress resistance in plants.

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“…The ability of EXPs to regulate cell wall modification and elongation means they have crucial functions in multiple biological processes, including response to biotic and abiotic stress, root and fiber development, root nodule formation, fruit development and ripening, and other developmental processes (Choi et al, 2006(Choi et al, , 2008Cosgrove, 2015b;Marowa et al, 2016). For instance, overexpression of the Osmanthus fragrans OfEXLA1 gene has been shown to increase resistance to salt and drought stress in Arabidopsis (Dong et al, 2023). Ectopic overexpression of wild Arachis AdEXLB8 in tobacco increased tolerances to biotic (Meloidogyne incognita and Sclerotinia sclerotiorum) and abiotic (drought) stresses (Brasileiro et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Abiotic stress treatment reveals expansin like A gene OfEXLA1 improving salt and drought tolerance of Osmanthus fragrans by responding to abscisic acid

Cited by 11 publications

References 37 publications

Genome-wide identification of the expansin gene family in netted melon and their transcriptional responses to fruit peel cracking

Genome-wide identification of the expansin gene family in netted melon and their transcriptional responses to fruit peel cracking

Transcriptome- and genome-wide systematic identification of expansin gene family and their expression in tuberous root development and stress responses in sweetpotato (Ipomoea batatas)

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