Abiotic transformations of iron and phosphate in humic lake water revealed by double‐isotope labeling and gel filtration

Haan, H. De; Jones, Roger; Salonen, Kalevi

doi:10.4319/lo.1990.35.2.0491

Cited by 54 publications

(43 citation statements)

References 4 publications

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“…6, only 10 % of the added label could not be accounted for as uptake into particulate fractions. This loss may either be a natural process converting orthophosphate to dissolved, but biologically unavailable forms such as adsorption to humic substances, complexation with Fe or other chemical processes (Cembella et al 1984b, Froelich, 1988, Fox, 1989, DeHaan & Salonen, 1990, or an artifact due to breakage of cells on the filters so that 10 %of the orthophosphate taken up was lost to the filtrate.…”

Section: Discussionmentioning

confidence: 99%

Phosphorus cycling and algal-bacterial competition in Sandsfjord, western Norway

Thingstad¹,

Skjoldal²,

Bohne³

1993

Mar. Ecol. Prog. Ser.

249

164

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Microbial phosphorus-cycling, with particular emphasis on algal/bacterial competition, was studied in depth profiles through the halocline separating a brackish top layer, rich in nitrate and poor in phosphate, from the underlying coastal water, poor in both nitrate and phosphate, in the Sandsfjord area, western Norway. At 2 stations along the axis of natural freshwater outflow, physiological P-deficiency of algae and bacteria in the brackish layer was inferred from rapid luxury consumption of added PO: -by organisms in size fractions > l pm and 1-0.2 pm, respectively. In a branch of the fjord without natural freshwater outlets, luxury consumption in the brackish layer was less, and without a clear difference between the 2 water layers. High luxury consumption coincided with short (< 30 min) turnover times for ~0 2 -a n d strong bacterial dominance of 32P0,"--uptake, suggesting bacterial superiority as competitors during P-limiting conditions. Estimation of P-specific maximum uptake rate and affinity for PO:--uptake from isotope dilution experiments indicated bacterial superiority at low and algal superiority at high concentrations. Although most of the 32P hydrolyzed from added y -~T 3 2 P was initially liberated as free 32P04, partitioning of incorporated 32P between size fractions > l pm and 1-0.2 pm was found to depend on whether label was added as y -~T " P or as 3 2~0 , , -, indicating that coupling of uptake to hydrolysis by cell-bound enzymes could modify the outcome of algalbacterial phosphorus competition. Disappearance rate of "P from the 1-0.2 pm size fraction following initial labeling and a subsequent cold chase with PO:-, was used to estimate the flow-rate of phosphorus through the microbial food web. Combined with measured kinetic constants for PO: -uptake. alkaline phosphatases and 5'nucleotidases, a coherent flow-scheme could only be obtained assuming very low (< 1 nmol I-') concentrations of PO;'-and nucleotides. Chemically measured concentrations of dissolved organic phosphorus (DOP) more than 2 orders of magnitude above the estimated nucleotide level and with an estimated turnover time of ca 500 h, are consistent with the view that this large Preservoir consists mainly of slowly hydrolyzable polymers.

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Section: Discussionmentioning

confidence: 99%

Phosphorus cycling and algal-bacterial competition in Sandsfjord, western Norway

Thingstad¹,

Skjoldal²,

Bohne³

1993

Mar. Ecol. Prog. Ser.

249

164

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“…The inhibition might have been partly due to the scavenging of hydroxyl radicals by HA (Christoforidis et al, 2015). In fact, the interactions between iron, phosphorus, light, and organic matter are very complex (Jones et al, 1998;De Haan et al, 1990). The glyphosate is one kind of stable organic phosphorus and the transformation of glyphosate to phosphate by Fe 3þ was inhibited by HA.…”

Section: Effect Of Humic Acidmentioning

confidence: 99%

“…A large quantity of phosphate would be fixed by Fe(III) by forming Fe(OOH)-P complexes or precipitates (Kleeberg et al, 2013), which inhibit the release of phosphorus. Meanwhile, the dissolved phosphate could be released from the phosphorus e humus -iron complex under UV irradiation (Francko andHeath, 1979, 1982;Jones et al, 1998;De Haan et al, 1990). The mechanisms were mainly concluded as following: (1) UV light can damage the phenol group of dissolved humus, which reduced affinity for dissolved iron, thereby promoting dissociation of phosphate; (2) Fe 3 þ of phosphorus e humus -iron complexes can obtain electronic from the dissolution of humus or other sources under UV light, resulting in the release of phosphorus.…”

Section: Introductionmentioning

confidence: 99%

The role of Fe(III) on phosphate released during the photo-decomposition of organic phosphorus in deionized and natural waters

et al. 2016

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“…sequent bioavailability of key nutrients (Francko and Heath 1979;De Haan et al 1990). There is substantial evidence for an association of phosphate and iron with DHS, and the quantities of iron and phosphate apparently associated with DHS in lake waters also vary with pH (Shaw et al 1992;Jones et al 1993).…”

Section: Notesmentioning

confidence: 99%

The effect of pH, dissolved humic substances, and ionic composition on the transfer of iron and phosphate to particulate size fractions in epilimnetic lake water

Shaw

1994

Limnology & Oceanography

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Transformations of dissolved iron and phosphate to particulate material (>0.2 µm) were investigated by adding 55FeCl3 and 32PO43 to pH adjusted epilimnetic lake water samples of contrasting ionic strengths and dissolved humic substances (DHS) concentrations. The distributions of these tracers between particulate size fractions in samples without appreciable DHS after 24 h of dark incubation varied markedly over the pH range 4–8. The effect of pH on the movement of 55Fe and 32P to particulate size fractions in clear‐water samples depended on the ionic strength. Over the same pH range, the presence of DHS suppressed the movement of 55Fe and 32P to particulate size fractions. There was little difference between low and high ionic strength DHS‐rich samples. The capability of DHS to maintain added 55Fe and 32P in solution (<0.2 µm) was confirmed by simultaneous measurements of precipitation losses.

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Abiotic transformations of iron and phosphate in humic lake water revealed by double‐isotope labeling and gel filtration

Cited by 54 publications

References 4 publications

Phosphorus cycling and algal-bacterial competition in Sandsfjord, western Norway

Phosphorus cycling and algal-bacterial competition in Sandsfjord, western Norway

The role of Fe(III) on phosphate released during the photo-decomposition of organic phosphorus in deionized and natural waters

The effect of pH, dissolved humic substances, and ionic composition on the transfer of iron and phosphate to particulate size fractions in epilimnetic lake water

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