2020
DOI: 10.21873/invivo.12045
|View full text |Cite
|
Sign up to set email alerts
|

Ablation Precision and Thermal Effects of a Picosecond Infrared Laser (PIRL) on Roots of Human Teeth: A Pilot Study Ex Vivo

Abstract: Background/Aim: Picosecond infrared laser (PIRL) was investigated regarding its possible therapeutic application in cutting dental roots. Materials and Methods: Extracted human teeth were processed in the root area by laser ablations followed by histological evaluation. Dentin adjacent to the cutting surface was evaluated morphometrically. Results: PIRL produced clearly defined cutting boundaries in dental roots. At the bottom of the cavity, the ablation surface became slightly concave. Heat development in … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3

Citation Types

0
3
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 10 publications
(3 citation statements)
references
References 63 publications
0
3
0
Order By: Relevance
“…One such ambient MS method utilizes the efficient coupling, on the picosecond time scale, between ablation and vibrational hydroxyl (−OH) stretch modes in water molecules (abundant in biological tissues) . Using pulses of mid-infrared laser energy with durations on the picosecond scale, a highly efficient desorption source is created that minimally damages the tissue outside the ablation zone, , requiring less than 1 mm 3 of tissue for a 10 s determination of various tissue pathologies through supervised multivariate modeling and comparisons with previously established, pathology-validated molecular libraries. ,, The method entitled picosecond infrared laser mass spectrometry (PIRL-MS) has been shown to recapitulate several morphometrically (as well as molecularly) distinct types and subtypes of cancers based on metabolic lipids statistically correlated to various pathologies. ,, PIRL-MS analysis, however, has not been examined for its potential utility in elucidating a specific mutation status from its correlated downstream metabolites. In this work, a set of metabolite ions associated with an oncogenic BRAF-V600E driver mutation were identified with untargeted PIRL-MS in a cell line model.…”
Section: Introductionmentioning
confidence: 99%
“…One such ambient MS method utilizes the efficient coupling, on the picosecond time scale, between ablation and vibrational hydroxyl (−OH) stretch modes in water molecules (abundant in biological tissues) . Using pulses of mid-infrared laser energy with durations on the picosecond scale, a highly efficient desorption source is created that minimally damages the tissue outside the ablation zone, , requiring less than 1 mm 3 of tissue for a 10 s determination of various tissue pathologies through supervised multivariate modeling and comparisons with previously established, pathology-validated molecular libraries. ,, The method entitled picosecond infrared laser mass spectrometry (PIRL-MS) has been shown to recapitulate several morphometrically (as well as molecularly) distinct types and subtypes of cancers based on metabolic lipids statistically correlated to various pathologies. ,, PIRL-MS analysis, however, has not been examined for its potential utility in elucidating a specific mutation status from its correlated downstream metabolites. In this work, a set of metabolite ions associated with an oncogenic BRAF-V600E driver mutation were identified with untargeted PIRL-MS in a cell line model.…”
Section: Introductionmentioning
confidence: 99%
“…Much like reports of utility of infrared lasers with larger pulse durations in MS, , a picosecond infrared laser sampling probe (ambient analysis) combined with MS (PIRL-MS) has recently shown utility in 10 s diagnosis of medulloblastoma subgroups with >95% sensitivity and specificity, and no signal carry over between measurements . This aerosolization is driven primarily through photomechanical mechanisms with minimal thermal damage and produces scar-free incisions that heal rapidly, so that it is well suited to skin cancer investigations in situ where cosmesis is important. In addition, as PIRL-MS requires only ∼1 mm 3 of tissue, downstream gross histopathologic evaluations of the laser sampled tissue is not drastically compromised .…”
Section: Introductionmentioning
confidence: 99%
“…However, current research on the use of lasers in dentistry has mainly focused on the microscopic morphology, thermal effect, and ablation efficiency of lasers on the dental hard tissues [ 14 , 28 , 29 , 30 , 31 ]. There have been few studies on the accuracy of robotically controlled femtosecond laser ablation in tooth preparation.…”
Section: Introductionmentioning
confidence: 99%