Abnormal activity of transcription factors gli in high-grade gliomas

Volnitskiy, Andrey; Shtam, Tatiana; Burdakov, Vladimir; Kovalev, R. A.; Konev, Alexander; Филатов, М. В.

doi:10.1371/journal.pone.0211980

Cited by 13 publications

(13 citation statements)

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“…TNC is overexpressed and correlated with tumor malignancy observed in many cancer types and is associated with glioma outcomes [34]. Previously, we have shown the increased levels of stem cell genes, including SOX2, OCT4, and GLI1 in these glioma cell lines [35,36] that coincide with the presence of the CD44 and TNC in all the exosome samples from CCM.…”

Section: Discussionmentioning

confidence: 88%

“…Secondly, it is a major vault protein (MVP), which is associated with nuclear pores, regulates nuclear-cytoplasmic transport and determines resistance to certain drugs [ 40 , 41 ]. It should be noted that its gene is a target of the transcription factor GLI1 [ 42 ], which according to our previous data is abnormally active in these glioma lines [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…The work was carried out using five glioma cell lines: A172 cell line obtained from the collection of the Institute of Cytology of the Russian Academy of Sciences (St. Petersburg, Russia) and glioma cell lines (Glia-Tr, Glia-L, Glia-R, and Glia-Sh) generated in the Laboratory of Cell Biology (NRC «Kurchatov Institute»-PNPI, Gatchina, Russia) [35,36]. The cells were cultured in an atmosphere of 5% CO 2 at 37 • C in DMEM/F12 (1:1) medium containing L-glutamine (BioloT, Saint Petersburg, Russia) and supplemented with 5% fetal bovine serum purified from exosomes by ultracentrifugation at 110,000× g for 18 h. The conditioned culture medium (CCM) was harvested after 72-96 h of incubation and growth of a cell monolayer in it.…”

Section: Glioma Cell Lines and Cultivation Conditionsmentioning

confidence: 99%

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Proteome of Glioblastoma-Derived Exosomes as a Source of Biomarkers

et al. 2020

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Extracellular vesicles (EV) are involved in important processes of glioblastoma multiforme (GBM), including malignancy and invasion. EV secreted by glioblastoma cells may cross the hematoencephalic barrier and carry molecular cargo derived from the tumor into the peripheral circulation. Therefore, the determination of the molecular composition of exosomes released by glioblastoma cells seems to be a promising approach for the development of non-invasive methods of the detection of the specific exosomal protein markers in the peripheral blood. The present study aimed to determine the common exosomal proteins presented in preparations from different cell lines and search potential glioblastoma biomarkers in exosomes. We have performed proteomics analysis of exosomes obtained from the conditioned culture medium of five glioblastoma cell lines. A list of 133 proteins common for all these samples was generated. Based on the data obtained, virtual two-dimensional electrophoresis (2DE) maps of proteins presented in exosomes of glioblastoma cells were constructed and the gene ontology (GO) analysis of exosome proteins was performed. A correlation between overexpressed in glial cell proteins and their presence in exosomes have been found. Thus, the existence of many potential glioblastoma biomarkers in exosomes was confirmed.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Glioma Cell Lines and Cultivation Conditionsmentioning

confidence: 99%

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Proteome of Glioblastoma-Derived Exosomes as a Source of Biomarkers

et al. 2020

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“…We hypothesized that the inherent glycosylation of such proteins and hydrolytic product (cellobiose) release from BC into biological system may be factors in triggering an immune response [73]. For this reason, we tested the biocompatibility of the enzyme using primary human glioblastoma available in local cell collection [46]. Glioblastoma cell line was chosen due to its highest inherent sensitivity towards toxic effects of a wide compound panel checked previously (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…For the cell viability test, human glioblastoma primary cell line # 15 from the laboratory cell culture collection [46] was used. Cells were cultured in DMEM/F-12 (1:1) medium, containing L-glutamine and supplemented with 10% fetal bovine serum (BioWest, Nuaillé, France) without antibiotics in 5% CO 2 at 37 • C. Cells were seeded in 4 parallel 96-well plates, seeding density was set at 1 × 10 4 cells per well.…”

Section: Cytotoxity Evaluationmentioning

confidence: 99%

Crystal and Supramolecular Structure of Bacterial Cellulose Hydrolyzed by Cellobiohydrolase from Scytalidium Candidum 3C: A Basis for Development of Biodegradable Wound Dressings

et al. 2020

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The crystal and supramolecular structure of the bacterial cellulose (BC) has been studied at different stages of cellobiohydrolase hydrolysis using various physical and microscopic methods. Enzymatic hydrolysis significantly affected the crystal and supramolecular structure of native BC, in which the 3D polymer network consisted of nanoribbons with a thickness T ≈ 8 nm and a width W ≈ 50 nm, and with a developed specific surface SBET ≈ 260 m2·g−1. Biodegradation for 24 h led to a ten percent decrease in the mean crystal size Dhkl of BC, to two-fold increase in the sizes of nanoribbons, and in the specific surface area SBET up to ≈ 100 m2·g−1. Atomic force and scanning electron microscopy images showed BC microstructure “loosening“after enzymatic treatment, as well as the formation and accumulation of submicron particles in the cells of the 3D polymer network. Experiments in vitro and in vivo did not reveal cytotoxic effect by the enzyme addition to BC dressings and showed a generally positive influence on the treatment of extensive III-degree burns, significantly accelerating wound healing in rats. Thus, in our opinion, the results obtained can serve as a basis for further development of effective biodegradable dressings for wound healing.

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