Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein β2 subunit gene and the Epo gene

Chrétien, Stany; Duprez, Véronique; Maouche, Leïla; Gisselbrecht, Sylvie; Mayeux, Patrick; Lacombe, Catherine

doi:10.1038/sj.onc.1201364

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…One of the cDNA encoded the G␤ 2 subunit of heterotrimeric G proteins and is the subject of the present study. It contained the entire coding sequence of the G␤ 2 subunit of heterotrimeric G proteins and the 5Ј noncoding sequence (53). We first wanted to exclude the possibility that the G␤ 2 clone was isolated because of the cross-reactivity of the antibodies used for expression cloning with a protein present in the immunogen but different from G␤ 2 .…”

Section: Screening Of a Bacterial Expression Library With Antibodies mentioning

confidence: 99%

Coupling of Heterotrimeric Gi Proteins to the Erythropoietin Receptor

Guillard¹,

Chrétien²,

Jockers³

et al. 2001

Journal of Biological Chemistry

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To identify new proteins involved in erythropoietin (Epo) signal transduction, we purified the entire set of proteins reactive with anti-phosphotyrosine antibodies from Epo-stimulated UT7 cells. Antisera generated against these proteins were used to screen a EXlox expression library. One of the isolated cDNAs encodes G␤ 2, the ␤ 2 subunit of heterotrimeric GTP-binding proteins. G␤ and G␣ i coprecipitated with the Epo receptor

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Section: Screening Of a Bacterial Expression Library With Antibodies mentioning

confidence: 99%

Coupling of Heterotrimeric Gi Proteins to the Erythropoietin Receptor

Guillard¹,

Chrétien²,

Jockers³

et al. 2001

Journal of Biological Chemistry

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“…A deletion on mouse chromosome 5 resulting in a rearrangement of the 5′ UTR of Gnb2 with part of exon 1 of Epo causes the abnormal erythropoietin gene expression found in murine erythroleukemic cell line IW32 (see GenBank: MUSERPB) (32). We find that this deletion removes a 45 kb region containing Rpp20, Perq1 and Gnb2 (Fig.…”

Section: Gnb2 Cgg Repeats and A Recombined Erythroleukemic Cell Linementioning

confidence: 67%

Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5

Wilson

Riemer²,

Martindale³

et al. 2001

Nucleic Acids Research

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Chromosome 7q22 has been the focus of many cytogenetic and molecular studies aimed at delineating regions commonly deleted in myeloid leukemias and myelodysplastic syndromes. We have compared a gene-dense, GC-rich sub-region of 7q22 with the orthologous region on mouse chromosome 5. A physical map of 640 kb of genomic DNA from mouse chromosome 5 was derived from a series of overlapping bacterial artificial chromosomes. A 296 kb segment from the physical map, spanning ACHE: to Tfr2, was compared with 267 kb of human sequence. We identified a conserved linkage of 12 genes including an open reading frame flanked by ACHE: and Asr2, a novel cation-chloride cotransporter interacting protein Cip1, Ephb4, Zan and Perq1. While some of these genes have been previously described, in each case we present new data derived from our comparative sequence analysis. Adjacent unfinished sequence data from the mouse contains an orthologous block of 10 additional genes including three novel cDNA sequences that we subsequently mapped to human 7q22. Methods for displaying comparative genomic information, including unfinished sequence data, are becoming increasingly important. We supplement our printed comparative analysis with a new, Web-based program called Laj (local alignments with java). Laj provides interactive access to archived pairwise sequence alignments via the WWW. It displays synchronized views of a dot-plot, a percent identity plot, a nucleotide-level local alignment and a variety of relevant annotations. Our mouse-human comparison can be viewed at http://web.uvic.ca/~bioweb/laj.html. Laj is available at http://bio.cse.psu.edu/, along with online documentation and additional examples of annotated genomic regions.

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Pyjacker identifies enhancer hijacking events in acute myeloid leukemia includingMNX1activation via deletion 7q

Sollier,

Riedel,

Toprak

et al. 2024

Preprint

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Acute myeloid leukemia with complex karyotype (ckAML) is characterized by high genomic complexity, including frequent TP53 mutations and chromothripsis. We hypothesized that the numerous genomic rearrangements could reposition active enhancers near proto-oncogenes, leading to their aberrant expression. We developed pyjacker, a computational tool for the detection of enhancer hijacking events, and applied it to a cohort of 39 ckAML samples. Pyjacker identified motor neuron and pancreas homeobox 1 (MNX1), a gene aberrantly expressed in 1.4% of AML patients, often as a result of del(7)(q22q36) associated with hijacking of a CDK6 enhancer. MNX1-activated cases show significant co-occurrence with BCOR mutations and a gene signature shared with t(7;12)(q36;p13) pediatric AML. We demonstrated that MNX1 is a dependency gene, as its knockdown in a xenograft model reduces leukemia cell fitness. In conclusion, enhancer hijacking is a frequent mechanism for oncogene activation in AML.

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Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein β2 subunit gene and the Epo gene

Cited by 3 publications

References 22 publications

Coupling of Heterotrimeric Gi Proteins to the Erythropoietin Receptor

Coupling of Heterotrimeric Gi Proteins to the Erythropoietin Receptor

Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5

Pyjacker identifies enhancer hijacking events in acute myeloid leukemia includingMNX1activation via deletion 7q

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