Abnormal expression of actin in lymphocytes of Alzheimer's disease and Down's syndrome patients

Jabbour, W.; Pouplard-Barthelaix, Annick; Houlgatte, Rémi; Emile, J.

doi:10.1016/0165-5728(92)90013-b

Cited by 18 publications

(12 citation statements)

References 32 publications

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“…β-actin expression has been shown to positively correlate with tumor invasiveness and metastatic potential [34], [35]. Altered expression of β-actin has also been observed in Alzheimer’s disease and Down’s syndrome patients [36], [37]. A recent study reported the translocation of β-actin from cytoplasm to nucleus during macrophage differentiation of HL-60 cells [38].…”

Section: Resultsmentioning

confidence: 99%

Housekeeping Gene Selection Advisory: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and β-Actin Are Targets of miR-644a

et al. 2012

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Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control. The possible direct or indirect effects of a miRNA on the expression of housekeeping genes are often overlooked. Among many housekeeping genes, expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin have been used extensively for normalization of gene expression data. Here, we show that GAPDH and β-actin are direct targets of miR-644a. Our data demonstrate the unsuitability of GAPDH and β-actin as internal controls in miR-644a functional studies and emphasize the need to carefully consider the choice of a reference gene in miRNA experiments.

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Section: Resultsmentioning

confidence: 99%

Housekeeping Gene Selection Advisory: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and β-Actin Are Targets of miR-644a

et al. 2012

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“…It is well known that ␤-actin encodes a cytoskeletal protein, the expression of which is altered in patients with AD and in those with Down's syndrome, possibly due to post-translational modifications of actin during disease progression (Jabbour et al, 1992). Similarly, the present data on differential expressions of 18S rRNA in AD patients are corroborated by a decrease in the mature ribosomal RNA 28S/18S ratio, which may be related to altered transcriptional processes in the ribosomal RNA maturation during AD progression (Da Silva et al, 2000;Payao et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…In general, these housekeeping genes are expressed in all nucleated cell-types and are necessary for cell survival (Butte et al, 2001;Warrington et al, 2000). However, some of these housekeeping genes, including actins, tubulins and 18S rRNA, are regulated in AD pathogenesis (Da Silva et al, 2000;Jabbour et al, 1992;Payao et al, 1994;Vijayan et al, 2001;Woulfe et al, 2002). Thus, it is critical to determine a suitable reference gene to calculate target gene mRNA copies or fold change in the brain specimens from AD patients.…”

Section: Introductionmentioning

confidence: 99%

The use of real-time PCR analysis in a gene expression study of Alzheimer’s disease post-mortem brains

Gutala

Reddy

2004

Journal of Neuroscience Methods

106

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“…The first 2-DE study in which cells of non-neuronal origin were investigated suggested an abnormal expression of actin in lymphocytes (67). Thereafter, we detected variations in proteomes of red blood cell membrane, platelets and lymphocytes between AD patients and controls (68).…”

Section: -De Studies In Admentioning

confidence: 98%

Problems Associated with Biological Markers of Alzheimer’s Disease

Frey¹,

Mattila

Korolainen

et al. 2005

Neurochem Res

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The etiopathogenesis of Alzheimer's disease (AD) is still unclear, although clinical diagnostic criteria exist and the neuropathology of AD has been studied in great detail during the last 20 years. The present study addresses certain problems in the search for biological markers for the diagnosis, as well as in the follow-up of the course of AD and its differential diagnosis and reports some of our own observations in comparison with other studies. These include protein, genetic and neuroimaging markers. The definitions of biological markers and search strategies are also discussed.

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Abnormal expression of actin in lymphocytes of Alzheimer's disease and Down's syndrome patients

Cited by 18 publications

References 32 publications

Housekeeping Gene Selection Advisory: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and β-Actin Are Targets of miR-644a

Housekeeping Gene Selection Advisory: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and β-Actin Are Targets of miR-644a

The use of real-time PCR analysis in a gene expression study of Alzheimer’s disease post-mortem brains

Problems Associated with Biological Markers of Alzheimer’s Disease

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