Abnormal Expression of the p53 Tumor Suppressor Gene in the Conjunctiva of Patients With Pterygium

Tan, Donald; Lim, Arthur; Goh, H. S.; Smith, Duncan R.

doi:10.1016/s0002-9394(14)70141-2

Cited by 104 publications

(70 citation statements)

References 4 publications

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“…We and other researchers (Dushku and Reid, 1997;Dushku et al, 1999;Tan et al, 1997Tan et al, , 2000Chowers et al, 2001;Ueda et al, 2001;Weinstein et al, 2002;Perra et al, 2006;Schneider et al, 2006) found pterygium cells to be p53 positive by IHC. The antip53 antibody and cutoff level (10% cell staining) that we used was the same as that used by Weinstein et al (2002) and by Tsai et al (2005b); however, the previously reported frequencies of 53.8 and 22.8%, respectively, differed from our finding of 24/36.…”

Section: Discussionsupporting

confidence: 56%

TP53 gene expression, codon 72 polymorphism and human papillomavirus DNA associated with pterygium

Rodrigues

Arruda²,

Silva³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Pterygium is a disease of unknown origin and pathogenesis that can be vision threatening. Several researchers believe that pterygium is UV-related and that abnormal expression of p53 protein and infection with human papillomavirus (HPV) are risk factors for pterygium, but their experiments have been inconclusive. We investigated its relation with p53 protein expression, p53 gene codon 72 polymorphism and infection with HPV DNA. Pterygial samples were obtained from 36 patients; 21 normal conjunctival samples were used as controls. Expression of p53 protein was studied by immunohistochemistry, using the antibody DO-7. Analysis for the p53 genotype was made by polymerase chain reaction, using specific primers for the arginine and proline alleles, and an analysis for HPV was made of the pterygium patients and control group. Fourteen of the 36 pterygial specimens were positive for abnormal p53 expression. Thirty-one of the patients were heterozygotic and three were homozygotic for the proline allele; two were homozygotic for the arginine allele; in the control group 12 of 21 were heterozy- gotic and seven of these 21 were homozygotic for the proline allele; two were homozygotic for the arginine allele. Twenty-one of the pterygium patients were positive for HPV; HPV type 1 was found in nine of these, type 2 in seven and both types in five. Only two of the 21 controls had HPV; both had type 16. We suggest that abnormal expression of p53, p53 codon 72 polymorphisms and HPV DNA are required co-factors for the development of pterygium.

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Section: Discussionsupporting

confidence: 56%

TP53 gene expression, codon 72 polymorphism and human papillomavirus DNA associated with pterygium

Rodrigues

Arruda²,

Silva³

et al. 2008

Genet. Mol. Res.

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“…89 The tumor-suppressor protein p53 is a widely studied factor in pterygia; normally present in low or undetectable levels within a cell, this protein functions to induce cell-cycle arrest, DNA repair or apoptosis. 90 In pterygia, p53 levels are increased 39,87,91 and several studies have reported abnormal p53 associated with HPV, 33,36 suggesting a role for viral oncoproteins in suppressing p53 activity. This contrasts with Dushku et al, 39 who detected increased p53 in pterygium without evidence of HPV infection, leading the authors to postulate that enhanced p53 was due to UV-induced mutagenesis.…”

Section: Role Of Hpv In P53 Tumor-suppressor Inactivationmentioning

confidence: 99%

Association of human papilloma virus with pterygia and ocular-surface squamous neoplasia

Girolamo

2011

Eye

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There are more microorganisms that colonize the human body than resident cells; some are commensal whereas others are pathogenic. Pathogenic microorganisms are sensed by the innate or adaptive immune system, an immune response is initiated, and the infection is often cleared. Some microorganisms have developed strategies to evade immune defenses, ensuring their long-term survival with potentially devastating consequences for the host. Approximately 18% of all cancers can be attributed to infective agents; the most common being Helicobacter pylori, Human papilloma virus (HPV) and Hepatitis B and C virus in causing stomach, cervical and liver carcinoma, respectively. This review focuses on whether HPV infection is necessary for initiating pterygia, a common benign condition and ocular-surface squamous neoplasia (OSSN), a rare disease with metastatic potential. The search engine PubMed was used to identify articles from the literature related to HPV and pterygium or conjunctival neoplasia. From 34 investigations that studied HPV in pterygia and OSSN, a prevalence rate of 18.6% (136/731) and 33.8% (144/426), respectively, was recorded. The variation in HPV prevalence (0-100%) for both disease groups may have arisen from study-design faults and the techniques used to identify the virus. Overall, the data suggest that HPV is not necessary for initiating either condition but may be a co-factor in susceptible hosts. Currently, over 60 million people worldwide have been immunized with HPV vaccines, but any effect on pterygium and OSSN development may not be known for some time as these lesions can evolve over decades or occur in older individuals.

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“…Histological studies in recent years have been associated pterygium with neoplastic proliferation. [5]Clinically it is seen in usuaaly old age , more common in males doing outdoor work, often bilateral.Usually present nasally.Presents with vision impairment due to induced astigmatism or progression into the pupillary areas of the cornea [6]. In the present study our goal was to highlight some clinical aspects and histopathological changes that occur in pterygium.…”

Section: Introductionmentioning

confidence: 99%

Pterygium: Clinical and Histopathological Study in Bundelkhand.

Sahay¹,

Kumar²,

Sharma³

2017

IOSR JDMS

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Abnormal Expression of the p53 Tumor Suppressor Gene in the Conjunctiva of Patients With Pterygium

Cited by 104 publications

References 4 publications

TP53 gene expression, codon 72 polymorphism and human papillomavirus DNA associated with pterygium

TP53 gene expression, codon 72 polymorphism and human papillomavirus DNA associated with pterygium

Association of human papilloma virus with pterygia and ocular-surface squamous neoplasia

Pterygium: Clinical and Histopathological Study in Bundelkhand.

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