Abnormal feeding behaviour in spinalised rats is mediated by hypothalamus: Restorative effect of exposure to extremely low frequency magnetic field

Abstract

SCI influences VMH, leading to alteration in feeding behaviour, which is improved by exposure to ELF-MF.

Extremely Low Frequency Magnetic Field Exposure Attenuates Pain By Inactivation Of Descending Facilitatory System In Complete Spinal Cord Injured Rats

“…Ltd, Mumbai, India) placed in alignment with the cut stumps of SC (SCI group), while only laminectomy without disrupting the dura was performed in Sham-SCI group. [14,15] The muscles and skin were sutured in layers with silicon thread (Ethicon, Johnson and Johnson Ltd, India) and topical antibiotic cream (Providon Iodine, Glide Chem Pvt Ltd, Rampur Ghat, India) was applied. Intra and post operative body temperature was maintained by placing the rat on a controlled heating pad (CMA-150, CMA Microdialysis, North Chelmsford, MA, USA).…”

“…[14] Briefly, it consists of four coils (two outer and two inner coils with 18 and 8 turns; respectively) mounted on a stand, a movable platform for the rat cage and a current regulator for maintaining constant current through the coils. The coils were connected in series to provide uniform MF on the central movable platform, [16] where six rats were placed in a specially designed polypropylene cage.…”

“…Behaving rat in open field arena (Video path analyzer, Coulbourn Instruments, USA) was video graphed and observed for BBB score criteria such as extent of joint movements, weight support ability and stepping/walking behaviour of the hind limbs for 5 min as described elsewhere. [14,21] Assessment of neuronal activity c-Fos immunohistochemistry Fos-immunoreactive (Fos-ir) profile in different areas of the brain was determined to assess their neuronal activity. The procedure of c-Fos immunohistochemistry is described elsewhere.…”

“…[6,11,12] More over, ELF-MF in rats has been reported from our laboratory to improve the quality of life after SCI by significantly improving locomotor recovery, autonomic control of urinary bladder, sensorimotor function, osteoporosis and eating behavior. [5,11,13,14] Recently, we have reported the facilitation of neuroregenerative processes also by it after SCI. [14] However, we are still ignorant about the influence of ELF-MF on pain modulation status after SCI.…”

“…[5,11,13,14] Recently, we have reported the facilitation of neuroregenerative processes also by it after SCI. [14] However, we are still ignorant about the influence of ELF-MF on pain modulation status after SCI. Therefore, the present study was proposed to investigate the effect of daily (2h for 8weeks) exposure to ELF-MF (17.96 µT, 50 Hz) on nociceptive responses and pain modulation status in spinalised rats.…”