Abnormal methylation patterns at the IGF2/H19 imprinting control region in phenotypically normal babies conceived by assisted reproductive technologies

Shi, Xiaodong; Ni, Yun-ping; Zheng, Haiyan; Chen, Shiling; Zhong, Ming; Wu, Fangrong; Xiao, Rong; Luo, Yan-Qun

doi:10.1016/j.ejogrb.2011.04.001

Cited by 32 publications

(18 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dietary factors, specifically folate supplementation in pregnant women, have been linked to reduced methylation of the imprinted IGF2 gene's ICRs in cord blood, an effect most pronounced in male infants (Hoyo et al, 2011). There is also some controversial literature on the impact of assisted reproductive technologies and in vitro fertilization on imprinting status, which has been suggested to be related to exposures experienced by the embryo during in vitro culture (DeBaun et al, 2003;Oliver et al, 2012;Puumala et al, 2012;Rancourt et al, 2012;Shi et al, 2011;Wong et al, 2011).…”

Section: Genomic Imprintingmentioning

confidence: 99%

Influence of environmental exposure on human epigenetic regulation

Marsit

2015

Journal of Experimental Biology

195

119

View full text Add to dashboard Cite

Environmental toxicants can alter epigenetic regulatory features such as DNA methylation and microRNA expression. As the sensitivity of epigenomic regulatory features may be greatest during the in utero period, when critical windows are narrow, and when epigenomic profiles are being set, this review will highlight research focused on that period. I will focus on work in human populations, where the impact of environmental toxicants in utero, including cigarette smoke and toxic trace metals such as arsenic, mercury and manganese, on genome-wide, gene-specific DNA methylation has been assessed. In particular, arsenic is highlighted, as this metalloid has been the focus of a number of studies and its detoxification mechanisms are well understood. Importantly, the tissues and cells being examined must be considered in context in order to interpret the findings of these studies. For example, by studying the placenta, it is possible to identify potential epigenetic adaptations of key genes and pathways that may alter the developmental course in line with the developmental origins of health and disease paradigm. Alternatively, studies of newborn cord blood can be used to examine how environmental exposure in utero can impact the composition of cells within the peripheral blood, leading to immunological effects of exposure. The results suggest that in humans, like other vertebrates, there is a susceptibility for epigenomic alteration by the environment during intrauterine development, and this may represent a mechanism of plasticity of the organism in response to its environment as well as a mechanism through which long-term health consequences can be shaped.

show abstract

Section: Genomic Imprintingmentioning

confidence: 99%

Influence of environmental exposure on human epigenetic regulation

Marsit

2015

Journal of Experimental Biology

195

119

View full text Add to dashboard Cite

show abstract

“…Normal methylation pattern was observed at SNRPN region in all 92 ICSI children examined [81], whereas hypo-methylation at KvDMR1 region was reported in three out of 18 clinically normal ART-conceived children [82]. Methylation status of the IGF2/H19 ICR in 61 phenotypically normal ART newborns was compared to 30 naturally conceived newborns and showed that overall methylation patterns were similar between the two groups [83]. In a more comprehensive analysis, 10 DMRs were analyzed in 77 ICSI, 35 IVF, and 73 natural conception children, and a slight hypermethylation was observed only at MEST region in IVF children [84].…”

Section: Impact Of Assisted Reproductive Technologies On Imprinting Amentioning

confidence: 99%

The impact of assisted reproductive technologies on genomic imprinting and imprinting disorders

Uyar

Seli

2014

Current Opinion in Obstetrics &Amp; Gynecology

View full text Add to dashboard Cite

Purpose of review Genomic imprinting refers to preferential allele-specific gene expression. DNA methylation-based molecular mechanisms regulate establishment and maintenance of parental imprints during early embryo development and gametogenesis. Because of the coincident timing, a potential association between assisted reproductive technology (ART) procedures and imprinting defects has been investigated in various studies. In this review, we provide an overview of genomic imprinting and present a summary of the relevant clinical data. Recent findings ART procedures affect DNA methylation pattern, parental imprinting status, and imprinted gene expression in the mouse embryo. In humans, several case series suggested an association between ART and imprinting disorders, with a three-fold to six-fold higher prevalence of ART use among children born with Beckwith–Wiedemann syndrome compared to the general population. However, more recent studies failed to support these findings and could not demonstrate an association between imprinting disorders and ARTs, independent of subfertility. Summary ART procedures may affect methylation status of imprinted regions in the DNA, leading to imprinting disorders. Although the low prevalence of imprinting disorders makes it challenging to perform conclusive clinical trials, further studies in large registries are required to determine the real impact of ARTs on their occurrence.

show abstract

“…Despite the fact that no case report or epidemiological study has been published reporting a major incidence of syndromes produced by methylation alterations in the paternal allele of children conceived by ART, the presence of anomalies in the imprinting of H19-ICR in samples of umbilical cord blood in three newborns out of 61 conceived by ART has recently been described. 45 It is noteworthy that (1) all three cases correspond to pregnancies of di-zygotic twins in which siblings did not present anomalies, (2) all three pregnancies were conceived by intracytoplasmic sperm injection (ICSI) because the fathers presented fertility problems and (3) none of the cases was identified as showing a Silver-Russell Syndrome phenotype, characteristic of this type of epigenetic anomaly.…”

Section: Methodsmentioning

confidence: 99%