Abnormal neutrophil scattergram obtained using Pentra MS CRP in the patients with myelodysplastic syndrome showing dysgranulopoiesis

Inaba, Tohru; Ishizuka, K.; Yuasa, S.; Mishima, Nozomu; Ogura, Kazuto; Saito, Kensuke; Kohno, T.; Fujitomo, Yumiko; Nakanishi, Masaki; Fujita, Naohisa

doi:10.1111/ijlh.12423

Cited by 8 publications

(8 citation statements)

References 23 publications

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“… Standardization of blood cell counting by the use of automated hematology analyzers Standardization of blood cell morphology Standardization of thrombosis and hemostasis …”

Section: Activities Of Jslh and Promotion Of The Standardization Of Lmentioning

confidence: 99%

Present status and perspective of laboratory hematology in Japan: On the standardization of blood cell morphology including myelodysplasia: On behalf of the Japanese Society for Laboratory Hematology

Tohyama

2018

Int J Lab Hematology

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The Japanese Society for Laboratory Hematology (JSLH) was launched in 2000 and has been developed by a mutual collaboration of hematologists, medical technologists, and the companies involved in hematological laboratory testing. The aim of JSLH is the progress and development of laboratory hematology by academic conferences, periodic publication of academic journal, training and education (in the meeting, the journal, or the website), promotion of the standardization of laboratory hematology, and certification of the laboratory hematology specialists. Among 3 specialized committees organized for the standardization of laboratory hematology, the standardization committee on blood cell morphology has been dealing with the various projects on peripheral/bone marrow blood cells and normal/abnormal morphology. Another independent organization, the Japanese National Research Group on idiopathic bone marrow failure syndromes (BMF), has raised the importance of the dysplasia of myelodysplastic syndromes (MDS) and has been conducting the prospective registration, central review, and follow-up study of MDS. This group recently proposed the grading system for diagnostic accuracy of MDS, and the detailed procedure of morphological diagnosis of MDS is presented in the specialized color atlas with typical photographs of various dysplasia. JSLH has also approved the grading system for diagnostic accuracy of MDS and adopted this standardized diagnostic system to the educational item for certification of the laboratory hematology specialists, aiming at a nationwidely expanding morphological evaluation of myelodysplasia. Further and significant progress in the standardization of blood cell morphology will be expected in Japan through the activity of JSLH.

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“… Standardization of blood cell counting by the use of automated hematology analyzers Standardization of blood cell morphology Standardization of thrombosis and hemostasis …”

Section: Activities Of Jslh and Promotion Of The Standardization Of Lmentioning

confidence: 99%

Present status and perspective of laboratory hematology in Japan: On the standardization of blood cell morphology including myelodysplasia: On behalf of the Japanese Society for Laboratory Hematology

Tohyama

2018

Int J Lab Hematology

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“…In this channel, flow impedance and light absorbance of respective cells are presented numerically from 0 to 127 channels (Ch.). Then, the mean light absorbance (MLA) of the cells within the NEU region is calculated to quantitatively evaluate neutrophil scattergrams [3] that results in 48.1 AE 3.6 Ch. (mean AE SD) for control individuals.…”

Section: Sirmentioning

confidence: 99%

Basic utility of Pentra series automated hematology analyzer for screening of Jordans’ anomaly

Inaba

Ishizuka

Suzuki

et al. 2016

Int J Lab Hematology

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“…[12][13][14][15] Although the hematology parameters obtained by these automated hematology analyzers have been previously used for the identification of MDS, most of them concerned complete blood count (CBC) analysis and neutrophil distribution patterns. [12][13][14][15] The analysis of BM aspirates using automated blood cell counters has revealed variable pros and cons, but appears to be a promising ancillary diagnostic approach with significant potential advantages. 16 Due to the fact that a BM aspirate will probably not be avoided for a definitive MDS diagnosis, it is the first time in this study that BM aspirates have been counted in common hematology analyzers and counts have been correlated with a "MDS" or "non-MDS" diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“Bone marrow aspirate automated counts on hematology analyzers: formulating a scoring system based on hematology parameters, to discriminate reactive versus myelodysplastic syndrome‐related bone marrows”

Tsagarakis¹,

Paterakis²,

Papadhimitriou³

et al. 2019

Int J Lab Hematology

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Introduction Diagnosis of myelodysplastic syndromes (MDS) is usually challenging. In this context, we have attempted to employ data derived from automated analysis of bone marrow (BM) samples as an ancillary tool for the discrimination between reactive marrow and MDS. Methods A total of 101 BM anticoagulated samples referred for flow cytometry (FCM) analysis on the clinical suspicion of MDS had been previously counted in a Mindray BC‐6800 hematology analyzer (testing set). Among them, 22/101 randomly selected BM samples (comparison set) had been also simultaneously counted by an Advia 2120 and a CELL‐DYN Sapphire hematology analyzer. Selected parameters obtained by Mindray BC‐6800 were retrospectively evaluated with ROC and regression analysis in an attempt to formulate a discriminative scoring system (SS) for MDS. This system was further evaluated in the comparison set. Results The diagnosis of MDS was established in 37/101 patients assessed (“MDS” group). Three patients were diagnosed with myelodysplastic/myeloproliferative neoplasm (MDS/MPN), while 61 revealed a “reactive” bone marrow (“RBM” group). Statistical analysis revealed significant differences in Hb, RDW‐CV%, NRBC%, and RET% values between the “MDS” and the “RBM” group. Specific cutoff values were then indicated and employed for the formulation of a SS of high sensitivity (86.84%) and specificity (86.89%). The encouraging performance characteristics of the proposed SS were also confirmed in the BM comparison set. Conclusion Automated BM counts on hematology analyzers contributed to the formulation of a SS for the screening discrimination between reactive and MDS BM fluids, which seems to be applicable and informative, regardless of the analyzer used.

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Abnormal neutrophil scattergram obtained using Pentra MS CRP in the patients with myelodysplastic syndrome showing dysgranulopoiesis

Abstract: Pentra MS CRP might rapidly generate useful information on dysplastic neutrophils in patients with MDS based on its cytochemistry for WBC differentials during routine laboratory hematology.

Cited by 8 publications

References 23 publications

Present status and perspective of laboratory hematology in Japan: On the standardization of blood cell morphology including myelodysplasia: On behalf of the Japanese Society for Laboratory Hematology

Present status and perspective of laboratory hematology in Japan: On the standardization of blood cell morphology including myelodysplasia: On behalf of the Japanese Society for Laboratory Hematology

Basic utility of Pentra series automated hematology analyzer for screening of Jordans’ anomaly

“Bone marrow aspirate automated counts on hematology analyzers: formulating a scoring system based on hematology parameters, to discriminate reactive versus myelodysplastic syndrome‐related bone marrows”

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