Abnormal NK Cell Lymphocytosis Detected after Splenectomy: Association with Repeated Infections, Relapsing Neutropenia, and Persistent Polyclonal B-Cell Proliferation

Granjo, Elisa; Lima, Margarida; Fraga, M.M.F.R.; Santos, Filipe; Magalhães, Conceição; Queirós, Maria Luı́s; Moreira, Ilidia M.; Rocha, Sandra; Santos-Silva, Alice; Rebelo, Irene; Quintanilha, Alexandre; Ribeiro, Maria Letı́cia; Candeias, J. A. N.; Órfão, Alberto

doi:10.1007/bf02982110

Cited by 4 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our finding of increased percentage and absolute numbers of NK cells is in agreement with studies published previously describing a long‐term increase of NK cell numbers/percentages in splenectomized patients after trauma or autoimmune thrombocytopenia [21–23]. This increase may contribute in some instances to side effects, such as the development of significant neutropenia, clinically manifested by repeated infections [24].…”

Section: Discussionsupporting

confidence: 92%

In Vitro Study of the Long‐Term Effects of Post‐Traumatic Splenectomy on Cellular Immunity

et al. 2004

View full text Add to dashboard Cite

The purpose of this study was to investigate the effect of splenectomy on cellular immunity. We studied the cellular phenotype and type 1 [interferon-g, interleukin-2 (IL-2)] and type 2 (IL-4 and IL-10) cytokine-producing peripheral blood CD4 þ and CD8 þ T lymphocytes in 22 healthy adults who had undergone post-traumatic splenectomy about 1 to 35 years ago. Splenectomy resulted in a long-term reduction of the percentage of CD4 þ CD45RAþ cells and a late increase of the percentage and absolute numbers of T-cell receptor g/d cells. Stimulation with Staphylococcal enterotoxin B resulted in normal IL-2 production by CD4þ T cells, indicating that the naïve cells were not anergic. Splenectomy also resulted in long-term priming of both CD4 þ and CD8 þ T cells. During the first 8 years, both type 1 and type 2 CD4 þ T cells were primed to varying degrees. About 8 years later, the percentage of primed type 2 CD4 þ T cells subsided, but that of type 1 CD4 þ T cells, although decreased, remained detectable over a longer period. Priming of CD8 þ T cells persisted throughout the study period. The long-term priming of type 1 CD4 þ and CD8 þ T cells, which may result in partial impairment of T-cell functions, may explain reported defects of immune responses to recall antigens in splenectomized individuals. In addition, changes in the profile of primed CD4þ T cells with time may be clinically relevant to relapses in autoimmune thrombocytopenia after splenectomy.

show abstract

Section: Discussionsupporting

confidence: 92%

In Vitro Study of the Long‐Term Effects of Post‐Traumatic Splenectomy on Cellular Immunity

et al. 2004

View full text Add to dashboard Cite

show abstract

“…(Ferrante et al, 1987;Wang et al, 1988;Balsalobre & Carbonell-Taday, 1991;Jahn et al, 1993;Wolf et al, 1999 (Kelemen et al, 1986;Demeter et al, 1990;Garcia-Suarez et al, 1995). Αυτή η αύξηση συσχετίζεται σε µερικές περιπτώσεις µε παρενέργειες όπως η ανάπτυξη σηµαντικής ουδετεροπενίας, που εκδηλώνεται κλινικά µε επανειληµµένες λοιµώξεις (Granjo et al, 2002).…”

Section: 34unclassified

Ο Ρόλος Του Σπλήνα Στην Ανοσορρύθμιση

Θεοδώρου¹

View full text Add to dashboard Cite

ΥΛΙΚΟ -ΜΕΘΟ∆ΟΙ ……………………………………………………..46 4.1 Υλικό -µέθοδοι ………………………………………………………….46 4.1.1 Υλικό …………………………………………………………………...46 4.1.2 Μορφολογική µελέτη επιχρισµάτων περιφερικού αίµατος …………….49 4.2 Μέθοδος προσδιορισµού ανοσοφαινότυπου επιφάνειας ………………49 4.3 Μέθοδος προσδιορισµού ενδοκυττάριων κυτταροκινών ……………..51 4.3.1 Κυτταρικές καλλιέργειες ……………………………………………….51 4.3.2 ∆ιαδικασία σήµανσης επιφάνειας και κυτταροπλάσµατος …………….52 4.3.3 Ανάλυση σε κυτταροµετρητή ροής …………………………………….52 4.4 Μέτρηση ενδοκυττάριων κυτταροκινών µετά από διέγερση µε το υπεραντιγόνο της σταφυλοκοκκικής εντεροτοξίνης Β ………………...54 4.4.1 Κυτταρικές καλλιέργειες ……………………………………………….54 4.4.2 ∆ιαδικασία σήµανσης επιφάνειας και κυτταροπλάσµατος …………….55 2 4.4.3 Ανάλυση σε κυτταροµετρητή ροής …………………………………….55 4.5 Μέτρηση κυτταροκινών σε καλλιέργειες µονοπυρήνων περιφερικού αίµατος µε ELISA (enzyme-linked immunoassay) …………56 4.5.1 Αποµόνωση και καλλιέργεια µονοπυρήνων περιφερικού αίµατος …….56 4.5.2 Μέτρηση των επιπέδων των κυτταροκινών µε ELISA ………………...57 ΚΕΦΑΛΑΙΟ 5 ……………………………………………………………...59 ΑΠΟΤΕΛΕΣΜΑΤΑ ……………………………………………………….59 5.1 Στατιστική ανάλυση …………………………………………………….59 5.2 Μορφολογική µελέτη επιχρισµάτων περιφερικού αίµατος …………..59 5.3 Μελέτη λεµφοκυτταρικών υποπληθυσµών περιφερικού αίµατος …...59 5.3.1 Απόλυτες τιµές και ποσοστά των λεµφοκυτταρικών υποπληθυσµών στα σπληνεκτοµηµένα άτοµα ………………………………..59 5.3.2 Χρόνος αποκατάστασης της κατανοµής των λεµφοκυτταρικών υποπληθυσµών του περιφερικού αίµατος ………………...60 5.3.3 ∆είκτες ενεργοποίησης Τ λεµφοκυτταρικών υποπληθυσµών ………….615.3.4 Απόλυτες τιµές και ποσοστά των λεµφοκυτταρικών υποπληθυσµών στα άτοµα µε συντηρητική αντιµετώπιση ρήξης σπληνός (ΣΑΡΣ) …………..62 5.4 Ενδοκυττάρια παραγωγή τύπου-1 και τύπου-2 κυτταροκινών ………67 5.4.1 Ενδοκυττάρια παραγωγή τύπου-1 και τύπου-2 κυτταροκινών χωρίς διέγερση στα σπληνεκτοµηµένα άτοµα ………………………………..67 5.4.2 Ενδοκυττάρια παραγωγή τύπου-1 και τύπου-2 κυτταροκινών χωρίς διέγερση στα άτοµα µε συντηρητική αντιµετώπιση ρήξης σπληνός …..68 5.4.3 Ενδοκυττάρια παραγωγή τύπου-1 και τύπου-2 κυτταροκινών µετά από µιτογονική διέγερση στα σπληνεκτοµηµένα άτοµα……………………...72 5.4.4 Ενδοκυττάρια παραγωγή τύπου-1 και τύπου-2 κυτταροκινών µετά από µιτογονική διέγερση στα άτοµα µε συντηρητική αντιµετώπιση ρήξης του σπλήνα ………………………………………………………...…..72 5.4.5 Ισορροπία του λόγου Τύπου-1/Τύπου-2 κυττάρων …………………….75 5.4.6 Ενδοκυττάρια παραγωγή των κυτταροκινών IFN-γ και IL-2 µετά από διέγερση µε το υπεραντιγόνο της σταφυλοκοκκικής εντεροτοξίνης Β ….77

show abstract

NK-cell neoplasms in Japan

et al. 2005

View full text Add to dashboard Cite

Neoplasms putatively originating from precursor and mature natural killer (NK) cells are rare, and their clinical features are unclear. A nationwide survey was performed in Japan to clarify the clinical features of these neoplasms diagnosed between 1994 and 1998, and data for 237 patients who met the criteria for putative NK cell-lineage neoplasms were analyzed. Among them, 11 had myeloid/NK-cell precursor acute leukemia, 15 blastic NK-cell lymphoma, 21 precursor NK-cell acute lymphoblastic leukemia, 22 aggressive NK-cell leukemia/lymphoma, 149 nasal-type NK-cell lymphoma (123 nasal and 26 extranasal) and 19 chronic NK lymphocytosis. The median overall survival time of patients with aggressive NK-cell leukemia/lymphoma was 2 months, which for chronic NK lymphocytosis was more than 8 years, and that for the other types of NK-cell neoplasms was between 6 and 22 months. Nasal NK-cell lymphoma and extranasal NK-cell lymphoma share the same histology. The age of affliction was the same, but the sex was different with males predominantly having nasal NK-cell lymphoma and females extranasal NK-cell lymphoma. Patients with extranasal NK-cell lymphoma had the tendency to exhibit a more advanced state of disease, with significantly higher International Prognostic Index and LDH levels, and significantly lower hemoglobin and platelet levels. The overall survival, however, did not differ significantly. Precursor NK-cell acute lymphoblastic leukemia and blastic NK-cell lymphoma were arbitrarily defined by the presence or absence of 30% or more of blastic cells in the bone marrow or peripheral blood, but there were no significant differences for affected age, gender, involved sites or prognosis. Aggressive NK-cell leukemia/lymphoma and extranasal NK-cell lymphoma were arbitrarily defined by the presence or absence of 30% or more of large granular lymphocytes in the bone marrow or peripheral blood and it is possible that aggressive NK-cell leukemia/lymphoma is a leukemic phase of extranasal NK-cell lymphoma. The incidence of skin involvement, however, was significantly higher for extranasal NK-cell lymphoma, suggesting that the two diseases are different. In nasal NK-cell lymphoma, Epstein-Barr virus in tumor cells was detected in all patients tested, suggesting its causative role.

show abstract

Abnormal NK Cell Lymphocytosis Detected after Splenectomy: Association with Repeated Infections, Relapsing Neutropenia, and Persistent Polyclonal B-Cell Proliferation

Cited by 4 publications

References 16 publications

In Vitro Study of the Long‐Term Effects of Post‐Traumatic Splenectomy on Cellular Immunity

In Vitro Study of the Long‐Term Effects of Post‐Traumatic Splenectomy on Cellular Immunity

Ο Ρόλος Του Σπλήνα Στην Ανοσορρύθμιση

NK-cell neoplasms in Japan

Contact Info

Product

Resources

About