Abnormal sarcoplasmic reticulum Ca<sup>2+</sup>-sequestering properties in skeletal muscle in chronic obstructive pulmonary disease

Green, Howard J.; Burnett, Margaret; Duhamel, Todd A.; D’Arsigny, Christine L.; O’Donnell, Denis E.; Ouyang, J.

doi:10.1152/ajpcell.00224.2008

Cited by 26 publications

(23 citation statements)

References 53 publications

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“…-ATPase measured microphometrically in the different fiber types. Unlike our finding in which we have reported reductions in the maximal catalytic activity in COPD when measured in whole muscle homogenates (Green et al 2008c), differences in the weighted Ca 2? -ATPase were not found when assessed histochemically.…”

Section: Discussioncontrasting

confidence: 99%

Muscle fiber type characteristics in females with chronic obstructive pulmonary disease. A preliminary study

et al. 2009

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Chronic obstructive pulmonary disease (COPD) is known to elicit intrinsic abnormalities in male skeletal muscle. However, it is unclear to what extent these changes occur in women and whether they are fiber-type specific. We investigated fiber-type specific differences in selected histochemical properties in muscle obtained from women with moderate to severe COPD compared to healthy control (CON) women. Tissue was obtained from the vastus lateralis in five COPD patients (age 66.9 +/- 2.6 years; FEV1 = 43 +/- 7%) and eight CON (age 68 +/- 4.9 years; FEV1 = 113 +/- 4.2%). Compared to CON, the distribution (30.6 +/- 5.2 vs. 57.9 +/- 4.6%) and cross sectional area of type I (CSA, 5660 +/- 329 vs. 3586 +/- 257 microm2) and type IIA (2770 +/- 302 vs. 2099 +/- 206 microm2) were lower (P < 0.05) and higher (P < 0.05), respectively, in COPD. Disease state did not alter either the distribution or CSA of the IIA, IIAX or type X subtypes. Although differences were found between fiber types in the number of capillary contacts (n) (I > IIAX, IIX; IIA > IIX) and the capillaries per CSA (microm210(-3)) (I < IIA, IIAX, IIX), no differences were found between CON and COPD. Succinic dehydrogenase activity and sarcoplasmic reticulum (SR) Ca2+-ATPase activity, measured photometrically (OD units), were higher (P < 0.05), and lower (P < 0.05), respectively, in type I compared to the type II fiber subtypes. These properties were not altered with COPD. COPD in females is accompanied by a higher percent of type II fibers, a larger CSA of type I and type IIA fibers, both of which occur in the absence of differences in oxidative potential and the potential for SR Ca2+-sequestration.

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Section: Discussioncontrasting

confidence: 99%

Muscle fiber type characteristics in females with chronic obstructive pulmonary disease. A preliminary study

et al. 2009

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“…Whether skeletal muscle SERCA levels and activity are also diminished has not been studied. Interestingly, in the skeletal muscle of patients with COPD, another chronic disease characterized by skeletal myopathy, SERCA activity was indeed found to be reduced, and calcium uptake was found to be slower compared to controls (24). These investigators postulated that the presence of reactive oxygen species (ROS) in skeletal muscle in COPD may lead to SERCA damage and dysfunction.…”

Section: What Are the Salient Features Of The “Skeletal Myopathy” Of mentioning

confidence: 99%

“…ROS–mediated damage to SERCA has been hypothesized as another potential mechanism of diminished calcium sequestration following calcium release from the SR. Other hyperadrenergic states, including COPD, also manifest this altered calcium cycling, leading to similar impairment of skeletal muscle function (24). …”

Section: What Are the Salient Features Of The “Skeletal Myopathy” Of mentioning

confidence: 99%

Making the Case for Skeletal Myopathy as the Major Limitation of Exercise Capacity in Heart Failure

Middlekauff

2010

Circ: Heart Failure

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“…Interestingly, although the high cost of SR Ca 2ϩ pumping during exercise is often not appreciated, SERCA dysfunction (i.e., slowing in the Ca 2ϩ uptake after contractions) has been implicated in many pathological states, including chronic obstructive pulmonary disease (13), muscular dystrophy (11), and heart failure (19). Because SERCA dysfunction may be an important factor associated with the decreased locomotor activity in these diseases (13), the study of the factors that influence SERCA activity during fatigue is very relevant.…”

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“…Because SERCA dysfunction may be an important factor associated with the decreased locomotor activity in these diseases (13), the study of the factors that influence SERCA activity during fatigue is very relevant.…”

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Ca²⁺-pumping impairment during repetitive fatiguing contractions in single myofibers: role of cross-bridge cycling

Nogueira

Shiah

Gandra

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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Nogueira L, Shiah AA, Gandra PG, Hogan MC. Ca 2ϩ -pumping impairment during repetitive fatiguing contractions in single myofibers: role of cross-bridge cycling. Am J Physiol Regul Integr Comp Physiol 305: R118 -R125, 2013. First published May 15, 2013 doi:10.1152/ajpregu.00178.2013.-The energy cost of contractions in skeletal muscle involves activation of both actomyosin and sarcoplasmic reticulum (SR) Ca 2ϩ -pump (SERCA) ATPases, which together determine the overall ATP demand. During repetitive contractions leading to fatigue, the relaxation rate and Ca 2ϩ pumping become slowed, possibly because of intracellular metabolite accumulation. The role of the energy cost of cross-bridge cycling during contractile activity on Ca 2ϩ -pumping properties has not been investigated. Therefore, we inhibited cross-bridge cycling by incubating isolated Xenopus single fibers with N-benzyl-p-toluene sulfonamide (BTS) to study the mechanisms by which SR Ca 2ϩ pumping is impaired during fatiguing contractions. Fibers were stimulated in the absence (control) and presence of BTS and cytosolic calcium ([Ca 2ϩ ]c) transients or intracellular pH (pHi) changes were measured. BTS treatment allowed normal [Ca 2ϩ ]c transients during stimulation without cross-bridge activation. At the time point that tension was reduced to 50% in the control condition, the fall in the peak [Ca 2ϩ ]c and the increase in basal [Ca 2ϩ ]c did not occur with BTS incubation. The progressively slower Ca 2ϩ pumping rate and the fall in pHi during repetitive contractions were reduced during BTS conditions. However, when mitochondrial ATP supply was blocked during contractions with BTS present (BTS ϩ cyanide), there was no further slowing in SR Ca 2ϩ pumping during contractions compared with the BTS-alone condition. Furthermore, the fall in pHi was significantly less during the BTS ϩ cyanide condition than in the control conditions. These results demonstrate that factors related to the energetic cost of cross-bridge cycling, possibly the accumulation of metabolites, inhibit the Ca 2ϩ pumping rate during fatiguing contractions. Ca 2ϩ uptake rate; skeletal muscle; cross-bridge cycling; fatigue; glycolysis; oxidative phophorylation DURING THE TRANSITION FROM rest to exercise in skeletal muscle, the demand for ATP increases several hundredfold, primarily because of the activation of both actomyosin (i.e., cross-bridge cycling) and sarcoplasmic reticulum (SR) Ca 2ϩ ATPase (SERCA) pumps (6). After membrane depolarization, cytosolic Ca 2ϩ concentration is transiently increased, thereby elevating actomyosin and SERCA ATPase activities. Although the Na ϩ -K ϩ -ATPase activity has a high contribution in the energy cost at rest [ϳ40% of total; (27)], it is quite small during contractions [ϳ1.5-7%; (6)]. Conversely, during contractions, actomyosin ATPase and SERCA account for ϳ60% and 40% of the total ATP demand, respectively (5,6,22,30). However, the energy cost of Ca 2ϩ handling by SERCA during contractions may be as high as 80% of the total energy cost in type IIB...

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Abnormal sarcoplasmic reticulum Ca²⁺-sequestering properties in skeletal muscle in chronic obstructive pulmonary disease

Cited by 26 publications

References 53 publications

Muscle fiber type characteristics in females with chronic obstructive pulmonary disease. A preliminary study

Muscle fiber type characteristics in females with chronic obstructive pulmonary disease. A preliminary study

Making the Case for Skeletal Myopathy as the Major Limitation of Exercise Capacity in Heart Failure

Ca²⁺-pumping impairment during repetitive fatiguing contractions in single myofibers: role of cross-bridge cycling

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Abnormal sarcoplasmic reticulum Ca2+-sequestering properties in skeletal muscle in chronic obstructive pulmonary disease

Cited by 26 publications

References 53 publications

Muscle fiber type characteristics in females with chronic obstructive pulmonary disease. A preliminary study

Muscle fiber type characteristics in females with chronic obstructive pulmonary disease. A preliminary study

Making the Case for Skeletal Myopathy as the Major Limitation of Exercise Capacity in Heart Failure

Ca2+-pumping impairment during repetitive fatiguing contractions in single myofibers: role of cross-bridge cycling

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Abnormal sarcoplasmic reticulum Ca²⁺-sequestering properties in skeletal muscle in chronic obstructive pulmonary disease

Ca²⁺-pumping impairment during repetitive fatiguing contractions in single myofibers: role of cross-bridge cycling