Abnormality of Very Long-Chain Fatty Acids of Erythrocyte Membrane in Alcoholic Patients

Adachi, Junko; Miwa, Atsuko; Ueno, Yoshio; Asano, Masaharu; Naito, Takeaki; Imamichi, Hiroyuki; Tatsuno, Yoshitsugu

doi:10.1097/00000374-199803001-00005

Cited by 10 publications

(3 citation statements)

References 3 publications

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“…Elevated levels of cerotic acid were previously reported in other diseases. Adachi et al [23] found higher levels of cerotic acid and hexacosenoic acid (26: 1) in erythrocyte membranes in alcoholic patients. According to these authors, this was a result of alcohol-induced interruption of peroxisomal beta-oxidation, a process involved in VLCFA metabolism [23].…”

Section: Discussionmentioning

confidence: 99%

“…Adachi et al [23] found higher levels of cerotic acid and hexacosenoic acid (26: 1) in erythrocyte membranes in alcoholic patients. According to these authors, this was a result of alcohol-induced interruption of peroxisomal beta-oxidation, a process involved in VLCFA metabolism [23]. Elevated serum levels of cerotic acid were also observed in X-linked adrenoleukodystrophy, an inherited disorder of peroxisomal metabolism characterized by deficient β-oxidation of saturated VLCFAs [24], as well as in other diseases associated with peroxisomal disorders [25].…”

Section: Discussionmentioning

confidence: 99%

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Hyper-Elongation in Colorectal Cancer Tissue – Cerotic Acid is a Potential Novel Serum Metabolic Marker of Colorectal Malignancies

Mika

Kobiela

Czumaj

et al. 2017

Cell Physiol Biochem

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Backgrounds/Aims: Colorectal cancer (CRC) cells show some alterations of lipid metabolism. Elongation of fatty acids (FA) has not been studied in CRC tissues thus far. The aim of this study was to verify if CRC specimens and normal colon mucosa differ in terms of their levels of very long-chain FAs, a product of FA elongation. Moreover, the expression of elongase genes has been studied in normal tissue and CRC. Finally, we searched for some specific products of FA elongation in serum of CRC patients. Methods: The specimens of normal colon mucosa and CRC were obtained from nineteen CRC patients differ in terms of FA elongation. We also searched for some specific products of FA elongation in serum of CRC patients and from healthy volunteers. Tissue and serum FA profiles were determined by means of gas chromatography-mass spectrometry (GC/MS), and the tissue expression of elongases (ELOVLs) was analyzed with real-time PCR. Results: Compared to normal colon tissue, CRC specimens showed significantly higher levels of 22-, 24- and 26-carbon FAs, stronger expressions of ELOVL1 and ELOVL6 (4- and 9-fold elevated respectively), and higher values of 18: 0/16: 0 elongation index. We also demonstrated presence of cerotic acid (26: 0) in serum of all CRC patients but in none of the healthy controls. Conclusions: CRC tissue seems to be characterized by enhanced FA elongation (hyper-elongation). Presence of cerotic acid in CRC patients sera and absence of this FA in healthy subjects points to this compound as a strong candidate for specific metabolic marker of colorectal malignancies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hyper-Elongation in Colorectal Cancer Tissue – Cerotic Acid is a Potential Novel Serum Metabolic Marker of Colorectal Malignancies

Mika

Kobiela

Czumaj

et al. 2017

Cell Physiol Biochem

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“…, 2006), post‐menopausal hormone replacement therapy (Stark et al. , 2003) and alcoholism (Adachi et al. , 1998) among these.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of a KCS gene from Cardamine graeca and its heterologous expression in Brassica oilseeds to engineer high nervonic acid oils for potential medical and industrial use

Taylor

Francis

Guo

et al. 2009

Plant Biotechnology Journal

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Database: The C. gracea KCS sequence information has been submitted to the EMBL ⁄ GenBank under accession number: bankit1110928.Keywords: 3-keto-acyl-CoA synthase, Cardamine graeca, Nervonic acid, Brassica carinata, Brassica napus, Arabidopsis thaliana, Health and Industry. SummaryNervonic acid 24:1 D15 (cis-tetracos-15-enoic acid) is a very long-chain monounsaturated fatty acid and exists in nature as an elongation product of oleic acid.There is an increasing interest in production of high nervonic acid oils for pharmaceutical, nutraceutical and industrial applications. Using a polymerase chain reaction approach, we have isolated a gene from Cardamine graeca L., which encodes a 3-ketoacyl-CoA synthase (KCS), the first component of the elongation complex involved in synthesis of nervonic acid. Expression of the Cardamine KCS in yeast resulted in biosynthesis of nervonic acid, which is not normally present in yeast cells.We transformed Arabidopsis and Brassica carinata with the Cardamine KCS under the control of the seed-specific promoter, napin. The T 3 generations of transgenic Arabidopsis and B. carinata plants expressing the Cardamine KCS showed that seed-specific expression resulted in relatively large comparative increases in nervonic acid proportions in Arabidopsis seed oil, and 15-fold increase in nervonic acid proportions in B. carinata seed oil. The highest nervonic acid level in transgenic B. carinata lines reached 44%, with only 6% of residual erucic acid. In contrast, similar transgenic expression of the Cardamine KCS in high erucic B. napus resulted in 30% nervonic acid but with 20% residual erucic acid. Experiments using the Lunaria KCS gene gave results similar to the latter. In both cases, the erucic acid content is too high for human or animal consumption. Thus, the Cardamine KCS: B. carinata high nervonic ⁄ highly reduced erucic transgenic seed oils will be the most suitable for testing in pharmaceutical ⁄ nutraceutical applications to improve human and animal health.

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Alcohol-induced Membrane Damage This work was supported in part by a Grant-in-Aid for Scientific Research from Japan Society for the Promotion of Science. We thank The British Council and the Daiwa Anglo-Japanese Foundation for funding the collaboration between the UK and Japan. We also thank Dr M. Asano, Dr R. Kudo, Dr T. Fujita, Dr K. Ishii, and Dr K. Ariyoshi (Kobe University) for help and discussion.

Adachi

Nushida

Ueno

et al. 2005

Comprehensive Handbook of Alcohol Related Pathology

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Abnormality of Very Long-Chain Fatty Acids of Erythrocyte Membrane in Alcoholic Patients

Cited by 10 publications

References 3 publications

Hyper-Elongation in Colorectal Cancer Tissue – Cerotic Acid is a Potential Novel Serum Metabolic Marker of Colorectal Malignancies

Hyper-Elongation in Colorectal Cancer Tissue – Cerotic Acid is a Potential Novel Serum Metabolic Marker of Colorectal Malignancies

Molecular cloning and characterization of a KCS gene from Cardamine graeca and its heterologous expression in Brassica oilseeds to engineer high nervonic acid oils for potential medical and industrial use

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