ABO blood group antigens of human spermatozoa.

Abstract

ABO blood group antigens on the membrane of human spermatozoa were investigated with the peroxidase-labelled antibody test. Spermatozoa from 0 secretor were incubated with saliva or seminal plasma of A and B secretors, but the specific peroxidase staining was negative. This result indicates that blood group antigens on human spermatozoa originate from spermatozoon itself, but not from seminal plasma. ABO blood group antigens ; human spermatozoa ; peroxidase-labelled antibody test Yamakami (1926) reported that spermatozoa have a specific inhibiting action on the human isoagglutinin, and in the same year Landsteiner and Levine (1926) demonstrated the presence of A and B blood group antigens on human spermatozoa using the inhibition technique. Thereafter, several investigators obtained similar results with various methods. Levine and Celano (1961), using the absorption-elution method, re-confirmed these results with spermatozoa from both secretor and non-secretor in an attempt to detect D (Rho) antigen in sperm cells. Shahani and Southam (1962), using the fluorescent antisera, supported the presence of blood group antigens on human spermatozoa. Recently, Inoue and Okada (1982, 1983) demonstrated ABO blood group antigens on the membrane of human spermatozoa using both enzyme and ferritin-labelled antibody techniques. However, there still remains a question whether ABO blood group antigens on the membrane of spermatozoa originate from seminal plasma or not. The present study deals with the origin of ABO antigens on human spermatozoa. MATERIALS and METHODSPreparation of samples. Semen samples were obtained from 13 donors of known blood groups (A secretor 7, B secretor 3, B non-secretor 1, AB secretor 1, and 0 secretor 1), and kept frozen at -40°C until use. One ml of the semen was washed twelve times in 10 ml of 0.01 M phosphate buffered saline (PBS), adjusted to pH 7.2, by centrifugation at 3,000 rpm for 10 min and the deposit was used as washed spermatozoa. The supernatant of twelfth washing was confirmed not to include water-soluble antigens, using either hemagglutination inhibition test or enzyme-labelled antibody test, the latter being performed by the method

ABH-related antigens in human male genital tract

“…It is basically accepted that ABH antigens can be detected on the spermatozoa extracted from seminal stains, However, it has long been a controversial subject whether the ABH antigens on the spermatozoa are inherent to the membrane or coating antigens. Takeda and Hiraiwa (1985) also reported that the adherence of A or B antigens from saliva or seminal fluid to O type spermatozoa was not observed after incubation in appropriate saliva or seminal fluid and the ABH antigens recognized on spermatozoa are inherent antigens of the sperm. Takeda and Hiraiwa (1985) also reported that the adherence of A or B antigens from saliva or seminal fluid to O type spermatozoa was not observed after incubation in appropriate saliva or seminal fluid and the ABH antigens recognized on spermatozoa are inherent antigens of the sperm.…”

Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification

“…In the present study, we tried to isolate sperm mixtures from multiple donors with different ABO blood group antigens. As ABO blood group antigens are located on sperm surface2425 and would facilitate the success of flow cytometry based sperm separation, therefore, it is imperative that we should first determine whether the genotype of semen is a secretor or non-secretor, followed by FACS separation to isolate type O, A, B, or AB sperm using ABO blood group antigen antibodies. It is known that the frequency of secretors: non-secretors is approximately 1:4, in spite of a slightly variation for different ancestry.…”

Heterogeneity in the presence of CD4-like molecules on human spermatozoa

“…Antigens specific only to spermatozoa and the seminal plasma as well as antigens common to the spermatozoa and other kinds of human cells have been identified and localized on human spermatozoa (Takeda & Hirava, 1985;Anderson et al, Correspondence: Associate Professor Dr Ts. Ts.…”