About the information depth of backscattered electron imaging

Piňos, Jakub; Mikmeková, Šárka; Frank, L.

doi:10.1111/jmi.12542

Cited by 21 publications

(14 citation statements)

References 18 publications

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“…In Figure , a comparison between the different components of the signal detected in electron microprobe analysis can be seen for a pure aluminium sample. The curves for backscattered electrons and primary photons exhibit the expected behaviour (Goldstein et al ., ; Piños et al ., ). At low energies, the signal source volume for secondary photons is clearly larger than the one corresponding to the other signals.…”

Section: Resultsmentioning

confidence: 97%

Lateral sensitivity in electron probe microanalysis studied by Monte Carlo simulations involving fluorescence enhancements

Petaccia

Castellano

2017

Journal of Microscopy

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In electron probe microanalysis, secondary fluorescence can occur leading to an increase of the volume analysed, degrading the lateral resolution of this technique. An adequate knowledge of the interaction volumes from where the different signals of interest are detected is determinant to estimate the minimum size of the zone that can be characterized. In this work, the size of the signal source volume is surveyed for a wide set of samples at different beam energies. To this aim, the PENELOPE software package was chosen to run Monte Carlo simulations for several experimental situations in order to produce the various lateral radiation distributions of interest. A comparison between the interaction volumes of the different signals was performed by taking into account the different fluorescence enhancement possibilities. An unexpected behaviour was found in the particular cases of aluminium and alumina, where the secondary photons signal exhibits a decreasing trend up to certain beam energy (∼17 keV); this implies that lower beam energies may degrade the lateral resolution of the technique in these materials.

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Section: Resultsmentioning

confidence: 97%

Lateral sensitivity in electron probe microanalysis studied by Monte Carlo simulations involving fluorescence enhancements

Petaccia

Castellano

2017

Journal of Microscopy

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“…The contrast in the image reflects contributions from the material composition, crystalline structure, and even electronic structure. A recent systematic BSE study of steel surface offers recommendations for routine estimations of BSE information depth on solid samples in the energy range of keV . More detailed analysis and comparison among analytical equations for information depth and the depth distribution function approach (eg, Casino Software) may be needed for future endeavor to fully understand the electron beam effect on image contrast in soft liquid materials.…”

Section: Resultsmentioning

confidence: 99%

Improving in situ liquid SEM imaging of particles

Arey

Chatterjee

et al. 2019

Surface & Interface Analysis

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This work presents in situ imaging of synthesized boehmite (γ‐AlOOH) particles ranging from 20 to 100 nm, suspended in liquid, in a vacuum compatible microfluidic sample holder using a scanning electron microscopy (SEM) under the high vacuum mode and highlights the advantage of in situ liquid imaging of colloids. Nanometer‐sized boehmite particles in high‐level radioactive wastes at the Hanford site are known to be difficult to dissolve and cause rheological problems for processing in the nuclear waste treatment plant. Therefore, it is important to characterize boehmite particles and understand how they form aggregates in the liquid state. Several technical advancements are made to optimize in situ liquid SEM chemical imaging resulting in the improved ability to obtain secondary electron (SE), backscattered electron (BSE) images, and energy dispersive X‐ray spectroscopy (EDX) spectra. Moreover, our results show mixed particles could be studied and identified based on the particle shape and elemental composition using in situ SEM imaging and EDX. Thus, we provide a new and improved approach to observe the evolution of particle dispersion and stability in liquid under conditions similar to those in the waste tank.

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“…As a result, heavier elements which backscatter more efficiently appear brighter than lighter elements in a backscattered electron image. Thus, a "brighter" BSE intensity correlates with greater average Z in the sample, and "dark" areas have lower average Z. BSE images are very helpful for obtaining high-resolution compositional maps of a sample and for quickly distinguishing different phases [37]. The representative BSE SEM micrographs of adipose samples from the PAT layer containing different concentration of iron are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, the lighter areas corresponding to accumulation, probably, of iron could be detected in the micrograph of the SAT sample without any traces of chromium. The depth of imaging with backscattered electrons in the scanning electron microscope, probably, should be measured to achieve a better interpretation of the obtained results [37]. Figure 8 shows three representative SEM BSE micrographs of the VAT layer of the adipose tissue samples having a very similar amount of chromium (5.3-5.5 μg/g) and taken from obese pa- tients having different metabolic diseases.…”

Section: Resultsmentioning

confidence: 99%

SEM (EDX) is an indispensable tool for the characterization of subcutaneous, preperitoneal and visceral adipose tissue of obese patients

Skaudžius¹,

Misevicius²,

Brimienė³

et al. 2018

chemija

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In this work the scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) elemental analysis were used for the characterization of adipose tissue samples taken from volunteer obese patients. The adipose tissue was taken from subcutaneous, preperitoneal and visceral layers of the adipose tissue. The obtained results provided information about the structural composition of adipose tissue layers in the human body, as well as the main microstructural features. It was demonstrated for the first time, to the best of our knowledge, that SEM and EDX are indispensable tools in order to investigate some special morphological features and the elemental composition of different layers of the human adipose tissue, identifying the surface microstructure and its qualitative composition. From the obtained results we concluded that such characterization of the adipose tissue is an essential step for the possible prediction of symptoms of different diseases.

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About the information depth of backscattered electron imaging

Cited by 21 publications

References 18 publications

Lateral sensitivity in electron probe microanalysis studied by Monte Carlo simulations involving fluorescence enhancements

Lateral sensitivity in electron probe microanalysis studied by Monte Carlo simulations involving fluorescence enhancements

Improving in situ liquid SEM imaging of particles

SEM (EDX) is an indispensable tool for the characterization of subcutaneous, preperitoneal and visceral adipose tissue of obese patients

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