A teleseismic profile consisting of 26 stations was deployed along 30°N latitude in the eastern Tibetan Plateau. By use of the inversion of P-wave receiver function, the S-wave velocity structures at depth from surface to 80 km beneath the profile have been determined. The inversion results reveal that there is significant lateral variation of the crustal structure between the tectonic blocks on the profile. From Linzhi north of the eastern Himalayan Syntaxis, the crust is gradually thickened in NE direction; the crustal thickness reaches to the maximum value (~72 km) at the Bangong-Nujiang suture, and then decreased to 65 km in the Qiangtang block, to 57-64 km in the Bayan Har block, and to 40-45 km in the Sichuan Basin. The eastern segment of the teleseismic profile (to the east of Batang) coincides geographically with the Zhubalong-Zizhong deep seismic sounding profile carried out in 2000, and the S-wave velocity structure determined from receiver functions is consistent with the P-wave velocity structure obtained by deep seismic sounding in respect of the depths of Moho and major crustal interfaces. In the Qiangtang and the Bayan Har blocks, the lower velocity layer is widespread in the lower crust (at depth of 30-60 km) along the profile, while there is a normal velocity distribution in lower crust in the Sichuan Basin. On an average, the crustal velocity ratio (Poisson ratio) in tectonic blocks on the profile is 1.73 (σ = 0.247) in the Lhasa block, 1.78 (σ = 0.269) in the Banggong-Nujiang suture, 1.80 (σ = 0.275) in the Qiangtang block, 1.86 (σ = 0.294) in the Bayan Har blocks, and 1.77 (σ = 0.265) in the Yangtze block, respectively. The Qiangtang and the Bayan Har blocks are characterized by lower S-wave velocity anomaly in lower crust, complicated Moho transition, and higher crustal Poisson ratio, indicating that there is a hot and weak medium in lower crust. These are considered as the deep environment of lower crustal flow in the eastern Tibetan Plateau. Flowage of the ductile material in lower crust may be attributable to the variation of the gravitational potential energy in upper crust from higher on the plateau to lower off plateau. eastern Tibetan Plateau, crustal and upper mantle's structure, teleseismic receiver function, lower crustal flowApplication of broadband seismic observation is one of the most marked advances in seismology. The high quality data recorded in broadband seismographs are used to study the deep media of the earth in several ways. Nowadays, it has become one of key techniques for exploring the parameters of the earth's structure and material physical property, such as velocity structure, Poisson