ABRE-BINDING FACTOR3-WRKY DNA-BINDING PROTEIN44 module promotes salinity-induced malate accumulation in pear

Alabd, Ahmed; Cheng, Haiyan; Ahmad, Mudassar; Wu, Xinyue; Peng, Lin; Wang, Lu; Yang, Shicai; Bai, Songling; Ni, Junbei; Teng, Yuanwen

doi:10.1093/plphys/kiad168

Cited by 20 publications

(4 citation statements)

References 70 publications

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“…TC-rich repeats are involved in defense and stress response [50]. In pear, ABRE-binding factor3 (PpABF3) promotes malate accumulation in response to salinity [51]. The JA signaling pathway participates in not only plant defense against abiotic and biotic stress, but also biosynthesis of flavonoids and anthocyanins, etc.…”

Section: Putative Functions Of Cqr2r3-myb Transcription Factorsmentioning

confidence: 99%

Genome-Wide Identification and Analysis of R2R3-MYB Genes Response to Saline–Alkali Stress in Quinoa

Liu

Wang

Huang

et al. 2023

IJMS

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Soil saline–alkalization inhibits plant growth and development and seriously affects crop yields. Over their long-term evolution, plants have formed complex stress response systems to maintain species continuity. R2R3-MYB transcription factors are one of the largest transcription factor families in plants, widely involved in plant growth and development, metabolism, and stress response. Quinoa (Chenopodium quinoa Willd.), as a crop with high nutritional value, is tolerant to various biotic and abiotic stress. In this study, we identified 65 R2R3-MYB genes in quinoa, which are divided into 26 subfamilies. In addition, we analyzed the evolutionary relationships, protein physicochemical properties, conserved domains and motifs, gene structure, and cis-regulatory elements of CqR2R3-MYB family members. To investigate the roles of CqR2R3-MYB transcription factors in abiotic stress response, we performed transcriptome analysis to figure out the expression file of CqR2R3-MYB genes under saline–alkali stress. The results indicate that the expression of the six CqMYB2R genes was altered significantly in quinoa leaves that had undergone saline–alkali stress. Subcellular localization and transcriptional activation activity analysis revealed that CqMYB2R09, CqMYB2R16, CqMYB2R25, and CqMYB2R62, whose Arabidopsis homologues are involved in salt stress response, are localized in the nucleus and exhibit transcriptional activation activity. Our study provides basic information and effective clues for further functional investigation of CqR2R3-MYB transcription factors in quinoa.

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Section: Putative Functions Of Cqr2r3-myb Transcription Factorsmentioning

confidence: 99%

Genome-Wide Identification and Analysis of R2R3-MYB Genes Response to Saline–Alkali Stress in Quinoa

Liu

Wang

Huang

et al. 2023

IJMS

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“…These results indicate that GsWRKY23 is involved in alleviating the ionic toxicity of overexpressed soybean and Arabidopsis plants under salt stress by regulating ionic uptake and transport in plants to adjust the ratios of Na + /K + and Cl − /NO 3 − for ionic homeostasis. Alabd et al reported that PpABF3 could target the G-box cis-element in the promoter of PpWRKY44, and PpWRKY44 directly bound to a W-box on the promoter of PpALMT9 to activate its expression and, thus, was positively involved in salinity-induced malate accumulation and pear fruit quality [42]. However, little is known about the downstream target genes and/or interacting proteins of GsWRKY23.…”

Section: Mechanisms Of the Gswrky23 Gene Conferred Salt Tolerance To ...mentioning

confidence: 99%

Functional Characterisation of the Transcription Factor GsWRKY23 Gene from Glycine soja in Overexpressed Soybean Composite Plants and Arabidopsis under Salt Stress

Sun,

Liu,

Zhang

et al. 2023

Plants

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WRKY proteins are a superfamily of transcription factors (TFs) that play multiple roles in plants’ growth, development, and environmental stress response. In this study, a novel WRKY gene called GsWRKY23 that is specifically upregulated in salt-tolerant Glycine soja accession BB52 seedlings was identified by transcriptomic analysis under salt stress. How the physiological functions and mechanisms of the GsWRKY23 gene affect salt tolerance was investigated using transformations of soybean hairy roots and Arabidopsis, including wild-type (WT) and atwrky23-mutant plants. The results showed that GsWRKY23 in the roots, stems, and leaves of BB52, along with its promoter in the cotyledons and root tips of GsWRKY23pro::GUS Arabidopsis seedlings, displayed enhanced induction under salt stress. GsWRKY23 localises to the nucleus and shows transcriptional activation ability in yeast cells. Compared to GsWRKY23-RNAi wild soybean hairy-root composite plants under salt stress, obvious improvements, such as superior growth appearance, plant height and fresh weight (FW), and leaf chlorophyll and relative water content (RWC), were displayed by GsWRKY23-overexpressing (OE) composite plants. Moreover, their relative electrolytic leakage (REL) values and malondialdehyde (MDA) contents in the roots and leaves declined significantly. Most of the contents of Na+ and Cl− in the roots, stems, and leaves of GsWRKY23-OE plants decreased significantly, while the content of K+ in the roots increased, and the content of NO3− displayed no obvious change. Ultimately, the Na+/K+ ratios of roots, stems, and leaves, along with the Cl−/NO3− ratios of roots and stems, decreased significantly. In the transgenic WT-GsWRKY23 and atwrky23-GsWRKY23 Arabidopsis seedlings, the salt-induced reduction in seed germination rate and seedling growth was markedly ameliorated; plant FW, leaf chlorophyll content, and RWC increased, and the REL value and MDA content in shoots decreased significantly. In addition, the accumulation of Na+ and Cl− decreased, and the K+ and NO3− levels increased markedly to maintain lower Na+/K+ and Cl−/NO3− ratios in the roots and shoots. Taken together, these results highlight the role of GsWRKY23 in regulating ionic homeostasis in NaCl-stressed overexpressed soybean composite plants and Arabidopsis seedlings to maintain lower Na+/K+ and Cl−/NO3− ratios in the roots and shoots, thus conferring improved salt tolerance.

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“…Malate is primarily produced in the cytosol and transported to the vacuole for storage as malic acid [42], an intermediate metabolite of the citric and glyoxylate cycles [43]. Many studies have shown that salt stress leads to the accumulation of malic acid in various plants, including Arabidopsis [43], Pear [44], apple [45], grape [46,47], rice [48], and tomato [49]. When plants are exposed to salinity stress, malic acid accumulates and is transported into mitochondria, where it feeds into the tricarboxylic acid cycle to promote mitochondrial ATP production and maintain respiratory flux [38,44,50].…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have shown that salt stress leads to the accumulation of malic acid in various plants, including Arabidopsis [43], Pear [44], apple [45], grape [46,47], rice [48], and tomato [49]. When plants are exposed to salinity stress, malic acid accumulates and is transported into mitochondria, where it feeds into the tricarboxylic acid cycle to promote mitochondrial ATP production and maintain respiratory flux [38,44,50]. Malate dehydrogenase (MDH) is a class of oxidoreductase enzymes that utilise NAD or NADP(H) as cofactors to catalyse the reversible reaction between malic acid and OAA [51].…”

Section: Introductionmentioning

confidence: 99%

OsMDH12: A Peroxisomal Malate Dehydrogenase Regulating Tiller Number and Salt Tolerance in Rice

Shi,

Feng,

Wang

et al. 2023

Plants

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Salinity is an important environmental factor influencing crop growth and yield. Malate dehydrogenase (MDH) catalyses the reversible conversion of oxaloacetate (OAA) to malate. While many MDHs have been identified in various plants, the biochemical function of MDH in rice remains uncharacterised, and its role in growth and salt stress response is largely unexplored. In this study, the biochemical function of OsMDH12 was determined, revealing its involvement in regulating tiller number and salt tolerance in rice. OsMDH12 localises in the peroxisome and is expressed across various organs. In vitro analysis confirmed that OsMDH12 converts OAA to malate. Seedlings of OsMDH12-overexpressing (OE) plants had shorter shoot lengths and lower fresh weights than wild-type (WT) plants, while osmdh12 mutants displayed the opposite. At maturity, OsMDH12-OE plants had fewer tillers than WT, whereas osmdh12 mutants had more, suggesting OsMDH12’s role in tiller number regulation. Moreover, OsMDH12-OE plants were sensitive to salt stress, but osmdh12 mutants showed enhanced salt tolerance. The Na+/K+ content ratio increased in OsMDH12-OE plants and decreased in osmdh12 mutants, suggesting that OsMDH12 might negatively affect salt tolerance through influencing the Na+/K+ balance. These findings hint at OsMDH12’s potential as a genetic tool to enhance rice growth and salt tolerance.

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ABRE-BINDING FACTOR3-WRKY DNA-BINDING PROTEIN44 module promotes salinity-induced malate accumulation in pear

Cited by 20 publications

References 70 publications

Genome-Wide Identification and Analysis of R2R3-MYB Genes Response to Saline–Alkali Stress in Quinoa

Genome-Wide Identification and Analysis of R2R3-MYB Genes Response to Saline–Alkali Stress in Quinoa

Functional Characterisation of the Transcription Factor GsWRKY23 Gene from Glycine soja in Overexpressed Soybean Composite Plants and Arabidopsis under Salt Stress

OsMDH12: A Peroxisomal Malate Dehydrogenase Regulating Tiller Number and Salt Tolerance in Rice

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