Abridged validation of charm II screening tests for the detection of veterinary drug residues in fish farmed in Cameroon

Takadong, Julie Judith Tsafack; Kamini, Mélanie Gondam; Mafo, Huguette Yangoua; Koupestchop, Evrard Medjo; Bouelet, Sandrine Isabelle Ntsama; Keutchatang, Fabrice De Paul Tatfo; Sasanya, James Jacob; Nama, Gabriel Medoua

doi:10.1080/19440049.2022.2107710

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…However, to satisfy the increasing demand for braised fish by consumers, antibiotics are abusively used in farming activities [2]. This has resulted in the presence of antibiotic residues in fish flesh [3] and thus the presence of multiple antibiotics-resistant microorganisms [4]. Hence, their consumption might lead to the development of antibiotics resistance.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Inhibition, Antibacterial and Antiadhesive Properties of a Novel Biosurfactant from Lactobacillus paracasei N2 against Multi-Antibiotics-Resistant Pathogens Isolated from Braised Fish

Mouafo,

Sokamte,

Manet

et al. 2023

Fermentation

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This study aimed to assess the antibiotic susceptibility and biofilm formation ability of pathogens isolated from braised fish as well as characterize and evaluate the antibacterial, antiadhesive, and antibiofilm activities of the biosurfactant from Lactobacillus paracasei subsp. tolerans N2 against these pathogens. The susceptibility of six pathogens isolated from braised fish (Escherichia coli EM2, Staphylococcus aureus SA1, Salmonella enteritidis PE1, Pseudomonas aeruginosa CT3, Yersinia enterolitica MH5, Proteus mirabilis MR2, and Klebsiella pneumoniae AG5) to 16 antibiotics revealed multiple resistances with an MAR index greater than 0.3. These pathogens were able to form biofilms with S. aureus SA1, which showed the highest ability. Using biochemical and elemental analyses, FTIR, GC-MS, 1H NMR and high-resolution mass spectrometry, the biosurfactant was characterized as a novel glycolipoprotein made of two congeners of mass 482.28 and 507.27 m/z, respectively. They showed bactericidal and antiadhesive activities against all pathogens. The biosurfactant inhibited biofilm formation by these pathogens and eradicated mature biofilms.

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Section: Introductionmentioning

confidence: 99%