Abrogation of cisplatin-induced nephrotoxicity in rats and HEK-293 cell lines by formononetin: in vivo and in vitro study

Shinde, Sachin D.; Jain, Pritam S.; Cheke, Rameshwar S.; Surana, Sanjay J.; Gunjegaonkar, S M

doi:10.1007/s00580-021-03252-x

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…These effects may be because of the higher antioxidant and FR scavenging potential of FN ( Dutra et al, 2021 ; Pan et al, 2021 ). Consistent with our findings, FN showed antioxidant activity and thus abrogated OS-mediated nephrotoxicity induced by various drugs such as cisplatin, MTX, and cyclophosphamide ( Aladaileh et al, 2019a ; Aladaileh et al, 2021b ; Shinde et al, 2021 ).…”

Section: Discussionsupporting

confidence: 91%

“…Various scientific reports have demonstrated that FN exhibits antioxidant, anti-inflammatory anti apoptotic, and tissue protective properties ( Aladaileh et al, 2019a ; Yi et al, 2020 ). It has been shown that FN improved renal function and demonstrated nephroprotective properties through ameliorating oxidative damage and lowering the degree of pro-inflammatory cytokines in vivo and in vitro models of cisplatin-induced nephrotoxicity ( Shinde et al, 2021 ). In addition, FN attenuated rhabdomyolysis-induced kidney apoptosis by upregulating Nrf2 ( Huang et al, 2016 ) .…”

Section: Introductionmentioning

confidence: 99%

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Formononetin Ameliorates Renal Dysfunction, Oxidative Stress, Inflammation, and Apoptosis and Upregulates Nrf2/HO-1 Signaling in a Rat Model of Gentamicin-Induced Nephrotoxicity

et al. 2022

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Gentamicin (GEN) is a bactericidal aminoglycoside known to cause nephrotoxicity. Formononetin (FN) is a potent flavonoid that exhibits numerous promising pharmacological activities. In this study, we have assessed the nephroprotective efficacy of FN against GEN-induced renal injury in rats. Rats were orally administered with FN (60 mg/kg/day, for 2 weeks) and were co-treated with intraperitoneal (i.p.) injection of GEN (100 mg/kg/day) during the days 8–14. GEN-treated rats demonstrated increased urea and creatinine levels in serum associated with marked histopathological changes in the kidney. Malondialdehyde (MDA) and protein carbonyl contents were elevated, whereas glutathione concentration and catalase and superoxide dismutase activities were lowered in GEN-administered rats. The FN largely prevented tissue damage, attenuated renal function, reduced MDA and protein carbonyl, and enhanced antioxidant capacity in the kidney of GEN-administrated animals. The kidney of GEN-treated rats demonstrated elevated Bax and caspase-3 protein expression, accompanied by lowered Bcl-2 protein expression, an effect that FN attenuated. Moreover, FN treatment caused upregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) expression in renal tissue of GEN-intoxicated animals. Collectively, FN protects against GEN-caused renal damage via exhibiting antioxidant, anti-inflammatory, and antiapoptotic activities and augmenting Nrf2 signaling, suggesting FN as a promising agent for preventing drug-induced organ damage.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Formononetin Ameliorates Renal Dysfunction, Oxidative Stress, Inflammation, and Apoptosis and Upregulates Nrf2/HO-1 Signaling in a Rat Model of Gentamicin-Induced Nephrotoxicity

et al. 2022

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“…After each animal's blood was taken by the retro-orbital method in Eppendorf tubes, the serum was separated by spinning at 10000 rpm for 10 minutes in a chilled centrifuge. The serum samples were stored at -20°C until analysis 14 .…”

Section: Collection Of Blood Samplesmentioning

confidence: 99%

“…The absorbance was measured at 540 nm. The amount of nitrite, which was represented as M/mg of protein, was calculated using a sodium nitrite (NaNO2) standard curve 14 .…”

Section: Determination Of Nitric Oxidementioning

confidence: 99%

Α-Asarone Protects CCL4 Induced Hepatotoxicity in Experimental Rats by inhibiting oxidative stress and cytokines

Tathe

Jat

Pathan

et al. 2022

J. Drug Delivery Ther.

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The present study aimed to study the protective effect of alpha- Asarone in CCL4-induced hepatotoxicity in experimental rats. The rats were randomly divided into 5 groups each containing six rats. CCL4 was given at a dosage of 0.5 mL/kg to produce hepatotoxicity, and serum AST, ALT, total bilirubin, and albumin, as well as hepatic hydroxyproline (HP), reduced glutathione (GSH), and malondialdehyde (MDA), cytokines, and NO, were assessed. CCL4 treatment resulted in a decrease in body weight and an increase in liver weight in rats, while treatment with α- Asarone resulted in normal body and liver weight. Serum AST, ALT, total bilirubin, HP, GSH, MDA, and cytokines were increased in CCL4 treated rats. α- Asarone-treated rats showed a reduction in oxidative stress as well as inhibited the release of cytokines in dose dependent manner and showed protection against hepatotoxicity. From the study, we conclude that, α- Asarone has a protective effect against the hepatotoxicity induced by CCL4. Keywords: CCL4; Hepatotoxicity; ALT; oxidative stress; α- Asarone; Cytokine.

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“…They demonstrated KIM‐1, calbindin, and TIMP‐1 as in vitro biomarkers for cisplatin‐induced nephrotoxicity using an HK‐2 human kidney cell system. Shinde et al (2021) studied a toxic effect of Cisplatin at 40.12% on HEK‐293 cells with ( p < .05) significance. HEK‐293 cells were isolated from human embryonic kidneys and extensively used cell line model of human renal cells in vitro research.…”

Section: Discussionmentioning

confidence: 99%

Nerolidol assists Cisplatin to induce early apoptosis in human laryngeal carcinoma Hep 2 cells through ROS and mitochondrial‐mediated pathway: An in vitro and in silico view

Balakrishnan

Sindhu

Vinothkumar

et al. 2022

Journal of Food Biochemistry

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The objective of this study was to examine Nerolidol (NER) and Cisplatin (CIS) performed against human laryngeal carcinoma (Hep 2) cells. We evaluated the effect of NER, CIS, and NER + CIS on cell viability, cell migration, oxidative stress, mitochondrial membrane depolarization, nuclear condensation, apoptotic induction, and DNA damage in Hep 2 cells. We used the MTT assay to assess the cytotoxicity effect of NER and CIS on Hep 2 cells in terms of morphological alterations. Present results demonstrated that IC50 values of NER and CIS have potential cytotoxicity against Hep 2 cells. NER effectively inhibited cell viability, increased reactive oxygen species generation, apoptotic induction, and DNA damage in Hep 2 cells. In addition, the docking study evaluated the structural binding interaction of NER with PI3K/Akt and PCNA protein. Furthermore, NER with PI3K/Akt, PCNA has a higher crucial score and affinity. Present results infer that NER could be used to target signaling molecules in anticancer studies. Practical applications Nerolidol is a dietary phytochemical with high biological activity that can find in a variety of plants. Many researchers focused on Nerolidol to treat various diseases including cancer. However, there is no studies exist on laryngeal cancer. This study uses Nerolidol and Cisplatin to generate oxidative stress and stimulate apoptosis and DNA damage in human laryngeal cancer cells. Based on present findings, Nerolidol could be a choice of anticancer medication, either alone or in combination against oral squamous cell carcinomas in both in vitro and in vivo experimental systems.

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Abrogation of cisplatin-induced nephrotoxicity in rats and HEK-293 cell lines by formononetin: in vivo and in vitro study

Cited by 6 publications

References 29 publications

Formononetin Ameliorates Renal Dysfunction, Oxidative Stress, Inflammation, and Apoptosis and Upregulates Nrf2/HO-1 Signaling in a Rat Model of Gentamicin-Induced Nephrotoxicity

Formononetin Ameliorates Renal Dysfunction, Oxidative Stress, Inflammation, and Apoptosis and Upregulates Nrf2/HO-1 Signaling in a Rat Model of Gentamicin-Induced Nephrotoxicity

Α-Asarone Protects CCL4 Induced Hepatotoxicity in Experimental Rats by inhibiting oxidative stress and cytokines

Nerolidol assists Cisplatin to induce early apoptosis in human laryngeal carcinoma Hep 2 cells through ROS and mitochondrial‐mediated pathway: An in vitro and in silico view

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