Abrogation of the proliferation of human leukemia cells in nude mice by a xenoantiserum

Infection of a human leukemia K-562 cell line with Semliki Forest virus

“…After initial infection, SFV-infected cells were subcultured at 1 week and then at weekly or tri-weekly intervals in RPMI 1640 medium supplemented with 15% NBS, Gentamycin (5 mcg/ml), Penicillin/Streptomycin (100 units/ml), and Fungizone (1 mcg/ml). These cells were shown to be free of mycoplasma and do not make interferon when stimulated with Newcastle Disease virus [25]. Chicken embryo fibroblasts (CEF), prepared from 10 day old embryos as primary cell cultures in medium 199 containing 2% fetal bovine serum were used for propagation of virus stocks and for plaque assays [19].…”

Absence of morphological, chromosomal and antigenic changes in the K-562 cell line growing as localized or disseminated tumours in nude mice

“…We have investigated whether morphological, chromosomal and antigenic changes occur in tumorigenic cells of human origin growing as localized s.c. or disseminated myelosarcomas (Lozzio et al, 1976aMachado et al, 1977Machado et al, , 1980 in immunodeficient mice. In our experiments, a continuous progeny of cells derived from a single aliquot of a culture passage of the K-562 cell line (Lozzio & Lozzio, 1975) (Lozzio et al, 1976a, b;Machado et al, 1977).…”

“…In our experiments, a continuous progeny of cells derived from a single aliquot of a culture passage of the K-562 cell line (Lozzio & Lozzio, 1975) (Lozzio et al, 1976a, b;Machado et al, 1977).…”

“…Morphological studies.-The growth of s.c. K-562 myelosarcomas (grossly visible 7-9 days after cell transplantation) was followed by measuring the external diameters twice a week with a caliper (Lozzio et al, 1976a, b;Machado et al, 1977). For recognition and recovery of pulmonary myelosarcomas, 3 mice were killed at intervals starting on the 14th and ending on the 120th day after neonatal injection of K-562 cells.…”

“…Antigenicity of K562 cells proliferating in vivo.-The presence of specific K-562-cell leukaemia antigen was determined by the antibody -dependent complement -mediated cytotoxicity of goat IgG to K-562 cells (Latif et al, 1979) on aliquots of cultured cells from s.c. and pulmonary myelosarcomas In addition, to demonstrate the specific antigens of K-562 cells proliferating in mice, sections of myelosarcomas were fixed in 10% formaldehyde in methanol, containing 0.3% H202 to inhibit mouse endogenous cell peroxidase and then processed as previously reported (Latif et al, 1979). The goat IgG was also absorbed with nudemouse lymphohaemopoietic cells (Latif et al, 1979 normal (pre-immune) IgG. The sequential steps of the experimental design are illustrated in Fig.…”