Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells

Kikuchi, Akira; Sanuki, Naoko; Higashi, Katsumi; Koshiba, Tomokazu; Kamada, Hiroshi

doi:10.1007/s00425-005-0114-y

Cited by 85 publications

(67 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sucrose treatment leads the explants to an inactive state, although it can still induce embryogenesis. Our previous study revealed that various stress treatments induce embryogenesis (Kikuchi et al 2006). The study also showed that the response of stress-treated explants is relatively slow.…”

Section: Discussionmentioning

confidence: 91%

“…For this reason, it has been postulated that embryogenic cells are already programmed for early-and late-stage embryogenesis at the molecular level, and it is difficult to efficiently isolate early embryonic genes using these cells (Kiyosue et al 1991(Kiyosue et al , 1993a(Kiyosue et al , 1993b. However, in the stress-induction system, the induction of embryonic gene expression corresponds to the acquisition of embryogenic competence during stress (Kikuchi et al 2006). The expression levels of early embryonic genes are thought to be enhanced under conditions of stress, and comparing expression profiles using the stress-induction system can help in isolating these genes.…”

Section: Discussionmentioning

confidence: 99%

“…Based on these observations, E-14-2 was expected to be an embryospecific gene with an important role in early embryogenesis. The frequency of somatic embryogenesis and the expression levels of C-ABI3 and ECPs increase with increasing duration of stress treatment (Kikuchi et al 2006). E-14-2 is specifically expressed in the heart and early torpedo-shaped stage of zygotic embryogenesis, but E-14-2 expression increases with increasing duration of the stress treatment that is used for the induction of somatic embryos, similar to the other isolated genes (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…To induce somatic embryos, sucrose-stress and auxin treatments were performed as described (Kikuchi et al 2006). For the sucrose-stress treatment, apical tip explants of 9-dayold-seedlings were cultured on phytohormone-free MS solid medium (0.8% agar, w/v) containing 0.7 M sucrose for 4 or 14 days at 25°C.…”

Section: Stress and Auxin Treatmentmentioning

confidence: 99%

“…In this induction system, longer stress treatments result in higher frequencies of somatic embryo formation, with stronger expression of embryo-specific genes occurring before morphological differentiation into embryos (Tachikawa et al 1998, Kikuchi et al 2006. As somatic cells of explants acquire embryogenic competence during stress treatment, and the degree of embryo formation is enhanced by increased treatment duration (Kikuchi et al 2006), some embryo-specific genes expressed in early embryogenesis are also likely to be expressed during the stress treatment, with their expression levels increasing with the duration of the stress treatment. Therefore, the carrot system was used in an attempt to isolate genes related to the early stage of embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Isolation of putative embryo-specific genes using stress induction of carrot somatic embryos

2009

Self Cite

View full text Add to dashboard Cite

Little is known about the physiological responses of higher plant cells during the early stages of embryogenesis and the genes that are involved in these responses. Carrot is an appropriate plant for the study of somatic embryogenesis as a model of zygotic embryogenesis. The system of stress induction of carrot somatic embryogenesis is useful for isolating genes expressed during embryogenesis, as embryogenic competence and the expression of certain embryo-specific genes increases with increased stress. Fluorescent differential display (FDD) was performed to isolate stress-induced genes in carrot somatic embryos, and five genes were isolated as embryonic tissue-specific genes. One of these genes was expressed until the early torpedo-shaped stage of zygotic embryogenesis. This result suggested that in the stress-induction system of carrot somatic embryos, embryo-specific genes expressed in the early stages of embryogenesis were induced during somatic embryo induction by stress treatment, concomitant with acquisition of embryonic competence. Unique genes expressed during early embryogenesis can be isolated from carrot by combining the appropriate experimental system and FDD. This strategy could be applied to many plant species for which genome information is not yet available.

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Stress and Auxin Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isolation of putative embryo-specific genes using stress induction of carrot somatic embryos

2009

Self Cite

View full text Add to dashboard Cite

show abstract

Plant Embryogenesis (Zygotic and Somatic)

Harada

Belmonte

Kwong

2010

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Zygotic embryogenesis in higher plants describes the developmental period in which the zygote undergoes a series of differentiation events, leading to the formation of a mature embryo. Establishment of the major embryonic organs and shoot and root apical meristems occur though partitioning events along the apical–basal axis, and many of these events are guided by the hormone auxin . Formation of the three embryonic tissue systems occurs along a radial axis perpendicular to the apical–basal axis. The mature zygotic embryo is generally developmentally arrested, metabolically quiescent and enclosed within maternal tissues of the seed. Somatic cells can be induced to divert from their normal fate and develop into embryos in a process termed somatic embryogenesis. Auxin and other plant hormones appear to play critical roles in inducing embryogenic competence. Zygotic and somatic embryogenesis represent parallel developmental programs in which cells acquire embryogenic cell fate and develop into mature embryos. Key Concepts: Embryo development can be divided into two phases: the morphogenesis phase in which the basic body plan of the embryo is established, and the maturation phase in which the embryo becomes tolerant of desiccation and accumulates storage macromolecules such as lipids, proteins and starch. During its development, the embryo is divided into distinct domains along its apical–basal and radial axes. Plant embryo formation represents a series of partitioning events in which organs and tissues are formed from larger domains. Cell fate within the embryo is largely dependent on the position of the cell within the embryo body. Plant cells are totipotent and have the ability to regenerate a fully differentiated organism. Somatic embryos follow similar developmental patterns to their zygotic counterparts, and they occur naturally or are induced in culture. Establishment of embryogenic competence occurs before the formation of the somatic embryo.

show abstract

Protocorm Regeneration and Protocorm-Like Bodies

Yeung,

Stasolla

2024

Springer Protocols Handbooks

View full text Add to dashboard Cite

Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells

Cited by 85 publications

References 42 publications

Isolation of putative embryo-specific genes using stress induction of carrot somatic embryos

Isolation of putative embryo-specific genes using stress induction of carrot somatic embryos

Plant Embryogenesis (Zygotic and Somatic)

Protocorm Regeneration and Protocorm-Like Bodies

Contact Info

Product

Resources

About