Abscisic acid binding to subcellular fractions from leaves of Vicia faba

Hocking, T. J.; Clapham, Jennifer; Cattell, K. J.

doi:10.1007/bf00386826

Cited by 33 publications

(15 citation statements)

References 6 publications

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“…6B) is consistent with earlier reports on the effect of pH on ABA function (11,15,52,65), showing that ABA was more effective at pH 7.3 than either acidic or alkaline pH. This is in contrast to the pH binding optimum of 5.3 found for ABA binding in apple fruit extracts (14).…”

Section: Ab2 Antibodies Recognize the Antigenic Determinant Of 15-i-c5contrasting

confidence: 34%

“…Attempts to study the early events of ABA action led to some success in describing cellular extracts with different ABA binding affinities (11)(12)(13)(14). The major impediment to isolating ABA-binding proteins has been attributed to their low abundance relative to other proteins, their sensitivity, and their association with insoluble cell components.…”

Section: Ab2 Antibodies Recognize the Antigenic Determinant Of 15-i-c5mentioning

confidence: 99%

“…There has been no success, unfortunately, in characterizing putative ABA receptors even with the use of genetic approaches (4). High affinity binding sites for ABA have been reported, however, in membrane fractions and guard cell plasmalemma of Vicia faba (11,12), microsomal fractions from Arabidopsis thaliana (13), and the cytosol of the developing flesh of apple fruits (14), and more recently, an ABA-specific binding protein was purified from the epidermis of broad bean leaves (15). Studies on ABA action in barley aleurones revealed two possible but different perception sites for ABA.…”

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confidence: 99%

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Purification and Characterization of a Barley Aleurone Abscisic Acid-binding Protein

Razem

Luo

Liu

et al. 2004

Journal of Biological Chemistry

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A protein designated ABAP1 and encoded by a novel gene (GenBank TM accession number AF127388) was purified and shown to specifically bind abscisic acid (ABA). ABAP1 protein is a 472-amino acid polypeptide containing a WW protein interaction domain and is induced by ABA in barley aleurone layers. Polyclonal antiidiotypic antibodies (AB2) cross-reacted with purified ABAP1 and with a corresponding 52-kDa protein associated with membrane fractions of ABA-treated barley aleurones. ABAP1 genes were detected in diverse monocot and dicot species, including wheat, tobacco, alfalfa, garden pea, and oilseed rape. Our data show that ABAP1 exerts high binding affinity for ABA. The interaction is reversible, follows saturation kinetics, and has stereospecificity, thus meeting the criteria for an ABA-binding protein.

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Section: Ab2 Antibodies Recognize the Antigenic Determinant Of 15-i-c5contrasting

confidence: 34%

Section: Ab2 Antibodies Recognize the Antigenic Determinant Of 15-i-c5mentioning

confidence: 99%

mentioning

confidence: 99%

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Purification and Characterization of a Barley Aleurone Abscisic Acid-binding Protein

Razem

Luo

Liu

et al. 2004

Journal of Biological Chemistry

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“…Chemical compounds such as ABA are generally thought to elicit a cascade of events by interacting with a specific receptor site(s). Saturable ABA binding sites have been described [66,67], but the corresponding proteins have not been further identified. In contrast, significant progress has been made in the identification of more downstream elements that contribute to the ABA regulation of ionic currents in stomatal guard cells or of gene expression in various tissues.…”

Section: Signal Transductionmentioning

confidence: 99%

Current advances in abscisic acid action and signalling

et al. 1994

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Abscisic acid (ABA) participates in the control of diverse physiological processes. The characterization of deficient mutants has clarified the ABA biosynthetic pathway in higher plants. Deficient mutants also lead to a revaluation of the extent of ABA action during seed development and in the response of vegetative tissues to environmental stress. Although ABA receptor(s) have not yet been identified, considerable progress has been recently made in the characterization of more downstream elements of the ABA regulatory network. ABA controls stomatal aperture by rapidly regulating identified ion transporters in guard cells, and the details of the underlying signalling pathways start to emerge. ABA actions in other cell types involve modifications of gene expression. The promoter analysis of ABA-responsive genes has revealed a diversity of cis-acting elements and a few associated trans-acting factors have been isolated. Finally, characterization of mutants defective in ABA responsiveness, and molecular cloning of the corresponding loci, has proven to be a powerful approach to dissect the molecular nature of ABA signalling cascades.

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“…The molecular mechanisms of abscisic acid action, in particular the underlying signal perception and transduction pathways, remain largely unknown. Saturable abscisic acid binding sites have been described (Hocking et al, 1978;Hornberg and Weiler, 1984), but the corresponding proteins have not been characterized. In stomatal guard cells, abscisic acid appears to play a direct role in regulating the rapid alteration of ion fluxes, whereas the remaining abscisic acid-mediated responses involving long-term changes appear to act at the leve1 of gene expression.…”

Section: Introductionmentioning

confidence: 99%

Isolation of the Arabidopsis ABI3 gene by positional cloning.

et al. 1992

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Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The AB13 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing an overlapping set of cosmid clones containing this marker, the abi3 locus was localized within a 35-kb region. An 11-kb subfragment was then shown to complement the mutant phenotype in transgenic plants, thereby further delimiting the position of the locus. A candidate AB13 gene was identified within this fragment as being expressed in developing fruits. The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone. In the most severe abi3-4 allele, the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon. The predicted AB13 protein displays discrete regions of high similarity to the maize viviparous-7 protein.

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Abscisic acid binding to subcellular fractions from leaves of Vicia faba

Cited by 33 publications

References 6 publications

Purification and Characterization of a Barley Aleurone Abscisic Acid-binding Protein

Purification and Characterization of a Barley Aleurone Abscisic Acid-binding Protein

Current advances in abscisic acid action and signalling

Isolation of the Arabidopsis ABI3 gene by positional cloning.

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